Literature DB >> 2569437

Production of discrete high specific activity DNA probes using the polymerase chain reaction.

R Jansen1, F D Ledley.   

Abstract

Conditions are described for the synthesis of discrete DNA probes with high specific activity using the polymerase chain reaction. This method enables the production of DNA probes between any two oligonucleotide sequences from cloned or uncloned templates. These probes are uniform in length and their specific activity (1 x 10(9) cpm/microgram) is comparable to probes produced by other methods.

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Year:  1989        PMID： 2569437     DOI： 10.1016/0735-0651(89)90020-4

Source DB:  PubMed          Journal:  Gene Anal Tech        ISSN： 0735-0651


  8 in total

Review 1.  Alterations in gene expression associated with primary demyelination and remyelination in the peripheral nervous system.

Authors:  A D Toews; J Hostettler; C Barrett; P Morell
Journal:  Neurochem Res       Date:  1997-10       Impact factor: 3.996

2.  The cytoplasmic domain of the large myelin-associated glycoprotein isoform is needed for proper CNS but not peripheral nervous system myelination.

Authors:  N Fujita; A Kemper; J Dupree; H Nakayasu; U Bartsch; M Schachner; N Maeda; K Suzuki; B Popko
Journal:  J Neurosci       Date:  1998-03-15       Impact factor: 6.167

3.  Oligodendrocytes and progenitors become progressively depleted within chronically demyelinated lesions.

Authors:  Jeffrey L Mason; Arrel Toews; Janell D Hostettler; Pierre Morell; Kinuko Suzuki; James E Goldman; Glenn K Matsushima
Journal:  Am J Pathol       Date:  2004-05       Impact factor: 4.307

4.  Comparison of the cytolysin II genetic determinants of Actinobacillus pleuropneumoniae serotypes.

Authors:  R Jansen; J Briaire; E M Kamp; M A Smits
Journal:  Infect Immun       Date:  1992-02       Impact factor: 3.441

5.  axl, a transforming gene isolated from primary human myeloid leukemia cells, encodes a novel receptor tyrosine kinase.

Authors:  J P O'Bryan; R A Frye; P C Cogswell; A Neubauer; B Kitch; C Prokop; R Espinosa; M M Le Beau; H S Earp; E T Liu
Journal:  Mol Cell Biol       Date:  1991-10       Impact factor: 4.272

6.  Structural analysis of the Actinobacillus pleuropneumoniae-RTX-toxin I (ApxI) operon.

Authors:  R Jansen; J Briaire; E M Kamp; A L Gielkens; M A Smits
Journal:  Infect Immun       Date:  1993-09       Impact factor: 3.441

7.  Expression of growth factor receptors, the focal adhesion kinase, and other tyrosine kinases in human soft tissue tumors.

Authors:  T M Weiner; E T Liu; R J Craven; W G Cance
Journal:  Ann Surg Oncol       Date:  1994-01       Impact factor: 5.344

8.  Genetic and molecular evidence for a trans-acting regulatory locus controlling glutathione S-transferase-2 expression in Aedes aegypti.

Authors:  D F Grant; B D Hammock
Journal:  Mol Gen Genet       Date:  1992-08
  8 in total

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