Literature DB >> 2569

The possibilities and limitations of membrane methods for the histochemical demonstration of cholinesterases.

Z Lukás, Z Fialová.   

Abstract

The thiocholine method for the histochemical detection of cholinesterases according to Karnovsky-Roots was adapted for unfixed cryostat sections by addition of the agar solution to the incubation mixture and by using the semipermeable membrane interposed between the section and the incubation medium. The procedure prevents the leakage of the enzyme activity of the section and is suitable for tissues where the cholinesterase activity is low.

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Year:  1976        PMID： 2569     DOI： 10.1007/BF02462788

Source DB:  PubMed          Journal:  Histochemistry        ISSN： 0301-5564


  12 in total

1.  A "DIRECT-COLORING" THIOCHOLINE METHOD FOR CHOLINESTERASES.

Authors:  M J KARNOVSKY; L ROOTS
Journal:  J Histochem Cytochem       Date:  1964-03       Impact factor: 2.479

2.  Effect of pH on the activity of eel esterase towards different substrates.

Authors:  F BERGMANN; S RIMON; R SEGAL
Journal:  Biochem J       Date:  1958-03       Impact factor: 3.857

3.  The use of hexazonium-p-rosanilin in the histochemical demonstration of peptidases.

Authors:  Z Lojda
Journal:  Histochemistry       Date:  1975-09-29

4.  Histochemical methods for acid beta-galactosidase: technics for semipermeable membranes.

Authors:  Z Lojda
Journal:  Histochemie       Date:  1973-12-31

5.  [Current problems in the cytochemistry of lysosomal hydrolases].

Authors:  Z Lojda
Journal:  Acta Morphol Acad Sci Hung       Date:  1972

6.  Semipermeable membranes for improving the histochemical demonstration of enzyme activities in tissue sections. 3. Lactate dehydrogenase.

Authors:  A E Meijer
Journal:  Histochemie       Date:  1973-05-22

7.  Semipermeable membranes for improving the histochemical demonstration of enzyme activities in tissue sections. IV. Glucose 6-phosphate dehydrogenase and 6-phosphogluconate dehydrogenase (decarboxylating).

Authors:  A E Meijer; G P de Vries
Journal:  Histochemistry       Date:  1974

8.  Lactic acid dehydrogenase isoenzymes of rat soleus muscle fibers as demonstrated by histochemical staining and electrophoresis.

Authors:  P J McMillan; R L Wittum
Journal:  J Histochem Cytochem       Date:  1971-07       Impact factor: 2.479

9.  Semipermeable membranes for improving the histochemical demonstration of enzyme activities in tissue sections. II. Nonspecific esterase and -glucuronidase.

Authors:  A E Meijer; A H Vloedman
Journal:  Histochemie       Date:  1973-02-06

10.  Histochemical demonstration of the intestinal hetero-beta-galactosidase (glucosidase).

Authors:  Z Lojda; E Havránková; J Slabý
Journal:  Histochemistry       Date:  1974
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  3 in total

Review 1.  The histochemistry of carboxylester hydrolases: problems and possibilities.

Authors:  H Luppa; J Andrä
Journal:  Histochem J       Date:  1983-02

2.  Improvement of the method of Karnovsky and Roots for the histochemical demonstration of acetylcholinesterase.

Authors:  P Kugler
Journal:  Histochemistry       Date:  1987

3.  A histochemical method for the demonstration of acetylcholinesterase activity using semipermeable membranes.

Authors:  J Andrä; Z Lojda
Journal:  Histochemistry       Date:  1986
  3 in total

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