Onauma Angwaravong1, Waranuch Pitiphat2, Jan G M Bolscher3, Ponlatham Chaiyarit4,5. 1. Department of Pediatric Dentistry, Faculty of Dentistry, Khon Kaen University, Khon Kaen, 40002, Thailand. 2. Department of Community Dentistry, Faculty of Dentistry, Khon Kaen University, Khon Kaen, 40002, Thailand. 3. Department of Oral Biochemistry, Academic Centre for Dentistry Amsterdam, University of Amsterdam and VU University Amsterdam, 1081 BT, Amsterdam, The Netherlands. 4. Department of Oral Diagnosis, Faculty of Dentistry, Khon Kaen University, Khon Kaen, 40002, Thailand. cponla@kku.ac.th. 5. Research Group of Chronic Inflammatory Oral Diseases and Systemic Diseases Associated with Oral Health, Faculty of Dentistry, Khon Kaen University, Khon Kaen, 40002, Thailand. cponla@kku.ac.th.
Abstract
OBJECTIVES: The aim of this study was to examine levels of salivary mucins in children with deciduous and mixed dentition and to determine correlations between salivary mucins and dental caries status in two dentition stages. MATERIALS AND METHODS: Saliva samples were collected from preschool children with deciduous dentition aged between 4 and 6 years (n = 60) and school children with mixed dentition aged between 9 and 11 years (n = 60). In each age group, the subjects were divided into two categories: high and low caries risk (n = 30 each). Salivary mucins (MUC5B and MUC7) were measured by enzyme-linked immunosorbent assay (ELISA). RESULTS: There were no significant differences in MUC5B and MUC7 levels between high and low caries-risk groups in preschool children. Significantly increased MUC5B (p = 0.01) and decreased MUC7 (p = 0.04) levels in a low caries-risk group were demonstrated in school children. No significant correlations were observed between salivary mucins and dental caries in preschool children, whereas a significantly negative correlation (r = -0.29, p = 0.03) between MUC5B and the number of decayed teeth was observed in school children. CONCLUSION: Patterns of salivary mucin expression in relation to dental caries were different between preschool and school children. The present findings suggest that changes in oral environment from deciduous to mixed dentition may affect the secretion of salivary mucins in response to dental caries. CLINICAL RELEVANCE: The present study provides additional information that changes in oral environment from deciduous to mixed dentition stage possibly affect the secretion of salivary mucins in response to dental caries.
OBJECTIVES: The aim of this study was to examine levels of salivary mucins in children with deciduous and mixed dentition and to determine correlations between salivary mucins and dental caries status in two dentition stages. MATERIALS AND METHODS: Saliva samples were collected from preschool children with deciduous dentition aged between 4 and 6 years (n = 60) and school children with mixed dentition aged between 9 and 11 years (n = 60). In each age group, the subjects were divided into two categories: high and low caries risk (n = 30 each). Salivary mucins (MUC5B and MUC7) were measured by enzyme-linked immunosorbent assay (ELISA). RESULTS: There were no significant differences in MUC5B and MUC7 levels between high and low caries-risk groups in preschool children. Significantly increased MUC5B (p = 0.01) and decreased MUC7 (p = 0.04) levels in a low caries-risk group were demonstrated in school children. No significant correlations were observed between salivary mucins and dental caries in preschool children, whereas a significantly negative correlation (r = -0.29, p = 0.03) between MUC5B and the number of decayed teeth was observed in school children. CONCLUSION: Patterns of salivary mucin expression in relation to dental caries were different between preschool and school children. The present findings suggest that changes in oral environment from deciduous to mixed dentition may affect the secretion of salivary mucins in response to dental caries. CLINICAL RELEVANCE: The present study provides additional information that changes in oral environment from deciduous to mixed dentition stage possibly affect the secretion of salivary mucins in response to dental caries.
Authors: Bing Liu; Jessica R Lague; David P Nunes; Paul Toselli; Frank G Oppenheim; Rodrigo V Soares; Robert F Troxler; Gwynneth D Offner Journal: J Histochem Cytochem Date: 2002-06 Impact factor: 2.479
Authors: J G Bolscher; J Groenink; J S van der Kwaak; P A van den Keijbus; W van 't Hof; E C Veerman; A V Nieuw Amerongen Journal: J Dent Res Date: 1999-07 Impact factor: 6.116
Authors: Galina Laputková; Vladimíra Schwartzová; Juraj Bánovčin; Michal Alexovič; Ján Sabo Journal: Open Life Sci Date: 2018-05-18 Impact factor: 0.938