| Literature DB >> 25686492 |
C Berrier1, R Peyronnet2, J-M Betton3, G Ephritikhine2, H Barbier-Brygoo2, J-M Frachisse2, A Ghazi4.
Abstract
Four different isoforms of the Voltage-Dependent Anion Channel (VDAC) have been identified in Arabidopsis plant cells. The electrophysiological characteristics of several VDAC channels from animal as well as plant cells are well documented, but those of this model plant are unknown. One isoform, AtVDAC-3 was obtained either directly by cell-free synthesis or produced in Escherichia coli, as inclusion bodies, and re-natured. An electrophysiological study of the purified proteins in planar lipid bilayers showed that both methods yielded proteins with similar channel activity. The characteristics of AtVDAC-3 are that of a bona fide VDAC-like channel.Entities:
Keywords: Arabidopsis; Cell-free synthesis; Channel; VDAC
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Year: 2015 PMID： 25686492 DOI： 10.1016/j.bbrc.2015.02.034
Source DB: PubMed Journal: Biochem Biophys Res Commun ISSN： 0006-291X Impact factor: 3.575