ETHNOPHARMACOLOGICAL RELEVANCE: Arrabidaea chica (Bignoniacea) has been used in popular medicine in Brazil to treat inflammation, skin diseases and leukemia. This work aimed to investigate the anti-inflammatory and antitumoral activities of the A. chica aqueous (AE) and ethanol (EE) extracts. MATERIALS AND METHODS: The murine sponge model was used to evaluate the anti-inflammatory and antiangiogenic activities of AE and EE. Accumulation of neutrophil and macrophage in the implants were determined by assaying myeloperoxidase and N-acetyl-glucosaminidase activities and the neovascularization evaluated by the amount of hemoglobin present in the implant using the Drabkin method. The antitumoral activity was evaluated using the MTT colorimetric method against Jurkat, HL60 and MCF-7 cells. Semi-purified fractions F1-F4 from the EE extract were obtained by a liquid-liquid solvent extraction method and their in vitro anti-proliferative effects were also investigated. RESULTS: Ethanol and aqueous extracts of A. chica decreased neutrophil accumulation and hemoglobin content in the sponge implants without altering the level of cytokines (IL-2, IL- 4, IL-5, IFN-γ, TNF-α and VEGF) and the albumin/globulin ratio in the serum of treated animals. There was no sign of toxicity (clinical, laboratory or histopathology). The ethanol extract presented antiproliferative activity (IC50 21.5-36.3 µg/mL) against HL60 and Jurkat cell lineages and proapoptotic activity at 50 µg/mL in HL60 cells. The fraction F1 also demonstrated significant antiproliferative activity (IC50 38.5 µg/mL) and proapoptotic activity against HL60 cells in a dose dependent manner. CONCLUSIONS: Aqueous and ethanol extracts of A. chica attenuate the inflammatory and angiogenic components of the subcutaneous fibrovascular tissue induced by the synthetic matrix in mice. In addition, the ethanol extract from Arrabidaea chica and its fraction F1 presented in vitro antiproliferative activity and could be useful for developing potential chemopreventive substances.
ETHNOPHARMACOLOGICAL RELEVANCE: Arrabidaea chica (Bignoniacea) has been used in popular medicine in Brazil to treat inflammation, skin diseases and leukemia. This work aimed to investigate the anti-inflammatory and antitumoral activities of the A. chica aqueous (AE) and ethanol (EE) extracts. MATERIALS AND METHODS: The murine sponge model was used to evaluate the anti-inflammatory and antiangiogenic activities of AE and EE. Accumulation of neutrophil and macrophage in the implants were determined by assaying myeloperoxidase and N-acetyl-glucosaminidase activities and the neovascularization evaluated by the amount of hemoglobin present in the implant using the Drabkin method. The antitumoral activity was evaluated using the MTT colorimetric method against Jurkat, HL60 and MCF-7 cells. Semi-purified fractions F1-F4 from the EE extract were obtained by a liquid-liquid solvent extraction method and their in vitro anti-proliferative effects were also investigated. RESULTS:Ethanol and aqueous extracts of A. chica decreased neutrophil accumulation and hemoglobin content in the sponge implants without altering the level of cytokines (IL-2, IL- 4, IL-5, IFN-γ, TNF-α and VEGF) and the albumin/globulin ratio in the serum of treated animals. There was no sign of toxicity (clinical, laboratory or histopathology). The ethanol extract presented antiproliferative activity (IC50 21.5-36.3 µg/mL) against HL60 and Jurkat cell lineages and proapoptotic activity at 50 µg/mL in HL60 cells. The fraction F1 also demonstrated significant antiproliferative activity (IC50 38.5 µg/mL) and proapoptotic activity against HL60 cells in a dose dependent manner. CONCLUSIONS: Aqueous and ethanol extracts of A. chica attenuate the inflammatory and angiogenic components of the subcutaneous fibrovascular tissue induced by the synthetic matrix in mice. In addition, the ethanol extract from Arrabidaea chica and its fraction F1 presented in vitro antiproliferative activity and could be useful for developing potential chemopreventive substances.
Authors: Cleydlenne Costa Vasconcelos; Alberto Jorge Oliveira Lopes; Emilly de Jesus Garcia Ataide; Kevin Waquim Pessoa Carvalho; Maria Fernanda Freitas de Brito; Marineide Sodré Rodrigues; Sebastião Vieira de Morais; Gyl Eanes Barros Silva; Claudia Quintino da Rocha; João Batista Santos Garcia; Maria do Socorro de Sousa Cartágenes Journal: Inflammopharmacology Date: 2021-04-21 Impact factor: 4.473
Authors: Alexandre Batista Penido; Selene Maia De Morais; Alan Bezerra Ribeiro; Daniela Ribeiro Alves; Ana Livya Moreira Rodrigues; Leonardo Hunaldo Dos Santos; Jane Eire Silva Alencar de Menezes Journal: Evid Based Complement Alternat Med Date: 2017-02-21 Impact factor: 2.629
Authors: Cleydlenne Costa Vasconcelos; Alberto Jorge Oliveira Lopes; Emerson Lucas Frazão Sousa; Darleno Sousa Camelo; Fernando César Vilhena Moreira Lima; Cláudia Quintino da Rocha; Gyl Eanes Barros Silva; João Batista Santos Garcia; Maria do Socorro de Sousa Cartágenes Journal: Int J Mol Sci Date: 2019-09-23 Impact factor: 5.923
Authors: Douglas C Brandão; Paula M A P Lima; Isabella C Martins; Carina S Cordeiro; Antonielle O Cordeiro; Lara Vecchi; Joyce F C Guerra; Priscila C Orsolin; Matheus C Gazolla; Danilo S Costa; Ademar A da Silva Filho; Thaise G Araújo Journal: BMC Complement Med Ther Date: 2022-01-20