Hai-Fei Yang1, Ai-Jun Pan2, Li-Fen Hu1, Yan-Yan Liu3, Jun Cheng1, Ying Ye4, Jia-Bin Li5. 1. Department of Infectious Disease, the First Affiliated Hospital of Anhui Medical University, Hefei, China. 2. Intensive Care Unit, Anhui Provincial Hospital, Hefei, China. 3. Institute of Bacterium Resistance, Anhui Medical University, Hefei, China; Anhui Center for Surveillance of Bacterial Resistance, Hefei, China. 4. Department of Infectious Disease, the First Affiliated Hospital of Anhui Medical University, Hefei, China; Institute of Bacterium Resistance, Anhui Medical University, Hefei, China; Anhui Center for Surveillance of Bacterial Resistance, Hefei, China. 5. Department of Infectious Disease, the First Affiliated Hospital of Anhui Medical University, Hefei, China; Institute of Bacterium Resistance, Anhui Medical University, Hefei, China; Anhui Center for Surveillance of Bacterial Resistance, Hefei, China; Department of Infectious Disease, Chaohu Hospital of Anhui Medical University, Hefei, China. Electronic address: younghaifei@gmail.com.
Abstract
BACKGROUND/ PURPOSE: Enterobacter cloacae is a well-recognized nosocomial pathogen. Use of a rapid, in vivo infection model for E. cloacae that can determine the efficacy of antibiotic therapies could help facilitate screening for new treatments. Nonmammalian model systems of infection, such as Galleria mellonella, have significant logistical and ethical advantages over mammalian models. MATERIALS AND METHODS: We utilized G. mellonella larvae to determine the utility of this infection model to study antibacterial efficacy. G. mellonella killing with heat-killed or live clinical isolates (E. cloacae GN1059 and GN0791) was tested. We also investigated the effect of postinoculation incubation temperature on the survival of infected larvae. The protection of administration of antibiotics to infected larvae was investigated. Finally, we determined the G. mellonella hemolymph burden of E. cloacae after administration of different antibiotics. RESULTS: With live bacterial inocula, G. mellonella killing was significantly dependent on the number of E. cloacae cells injected in a dose-dependent manner. Further, we observed that survival was reduced with increasing the postinoculation temperature. Treatment of a lethal E. cloacae infection with antibiotics that had in vitro activity significantly prolonged the survival of larvae compared with treatment with antibiotics to which the bacteria were resistant. The therapeutic benefit arising from administration of antibiotic correlated with a reduced burden of E. cloacae cells in the hemolymph. CONCLUSION: The G. mellonella infection model has the potential to be used to facilitate the in vivo study of host-pathogen interactions in E. cloacae and the efficacy of antibacterial agents.
BACKGROUND/ PURPOSE:Enterobacter cloacae is a well-recognized nosocomial pathogen. Use of a rapid, in vivo infection model for E. cloacae that can determine the efficacy of antibiotic therapies could help facilitate screening for new treatments. Nonmammalian model systems of infection, such as Galleria mellonella, have significant logistical and ethical advantages over mammalian models. MATERIALS AND METHODS: We utilized G. mellonella larvae to determine the utility of this infection model to study antibacterial efficacy. G. mellonella killing with heat-killed or live clinical isolates (E. cloacae GN1059 and GN0791) was tested. We also investigated the effect of postinoculation incubation temperature on the survival of infected larvae. The protection of administration of antibiotics to infected larvae was investigated. Finally, we determined the G. mellonella hemolymph burden of E. cloacae after administration of different antibiotics. RESULTS: With live bacterial inocula, G. mellonella killing was significantly dependent on the number of E. cloacae cells injected in a dose-dependent manner. Further, we observed that survival was reduced with increasing the postinoculation temperature. Treatment of a lethal E. cloacaeinfection with antibiotics that had in vitro activity significantly prolonged the survival of larvae compared with treatment with antibiotics to which the bacteria were resistant. The therapeutic benefit arising from administration of antibiotic correlated with a reduced burden of E. cloacae cells in the hemolymph. CONCLUSION: The G. mellonella infection model has the potential to be used to facilitate the in vivo study of host-pathogen interactions in E. cloacae and the efficacy of antibacterial agents.
Authors: Livia Aparecida Procópio Gomes; Lívia Mara Alves Figueiredo; Ana Luiza do Rosário Palma; Barbara Maria Corrêa Geraldo; Kelly Cristine Isler Castro; Luciana Ruano de Oliveira Fugisaki; Antônio Olavo Cardoso Jorge; Luciane Dias de Oliveira; Juliana Campos Junqueira Journal: ScientificWorldJournal Date: 2016-09-07
Authors: Aiste Dijokaite; Maria Victoria Humbert; Emma Borkowski; Roberto M La Ragione; Myron Christodoulides Journal: Virulence Date: 2021-12 Impact factor: 5.882