Richard A Anderson1, Ellen Anckaert2, Ernesto Bosch3, Didier Dewailly4, Cheryl E Dunlop5, Daniel Fehr6, Luciano Nardo7, Johan Smitz2, Kelton Tremellen8, Barbara Denk9, Andrea Geistanger9, Martin Hund10. 1. MRC Centre for Reproductive Health, University of Edinburgh, Queen's Medical Research Institute, Edinburgh, United Kingdom. Electronic address: richard.anderson@ed.ac.uk. 2. Laboratory of Hormonology and Tumour Markers, Universitair Ziekenhuis Brussel, Free University of Brussels (VUB), Brussels, Belgium. 3. IVI Valencia, Valencia, Spain. 4. Department of Endocrine Gynaecology and Reproductive Medicine, Hôpital Jeanne de Flandre, Centre Hospitalier de Lille, Université Lille 2, Lille, France. 5. MRC Centre for Reproductive Health, University of Edinburgh, Queen's Medical Research Institute, Edinburgh, United Kingdom. 6. UniKiD, Univ.-Frauenklinik Düsseldorf, Düsseldorf, Germany. 7. GyneHealth Reproductive Health Group, Manchester, United Kingdom. 8. Repromed, Dulwich, Australia. 9. Roche Diagnostics GmbH, Penzberg, Germany. 10. Roche Diagnostics International Ltd., Rotkreuz, Switzerland.
Abstract
OBJECTIVE: To evaluate a new fully automated assay measuring antimüllerian hormone (AMH; Roche Elecsys) against antral follicle count in women of reproductive age. DESIGN: Prospective cohort study. SETTING: Hospital infertility clinics and academic centers. PATIENT(S): Four hundred fifty-one women aged 18 to 44 years, with regular menstrual cycles. INTERVENTION(S): None. MAIN OUTCOME MEASURE(S): AMH and antral follicle count (AFC) determined at a single visit on day 2-4 of the menstrual cycle. RESULT(S): There was a statistically significant variance in AFC but not in AMH between centers. Both AFC and AMH varied by age (overall Spearman rho -0.50 for AFC and -0.47 for AMH), but there was also significant between-center variation in the relationship between AFC and age but not for AMH. There was a strong positive correlation between AMH and AFC (overall spearman rho 0.68), which varied from 0.49 to 0.87 between centers. An agreement table using AFC cutoffs of 7 and 15 showed classification agreement in 63.2%, 56.9% and 74.5% of women for low, medium, and high groups, respectively. CONCLUSION(S): The novel fully automated Elecsys AMH assay shows good correlations with age and AFC in women of reproductive age, providing a reproducible measure of the growing follicle pool.
OBJECTIVE: To evaluate a new fully automated assay measuring antimüllerian hormone (AMH; Roche Elecsys) against antral follicle count in women of reproductive age. DESIGN: Prospective cohort study. SETTING: Hospital infertility clinics and academic centers. PATIENT(S): Four hundred fifty-one women aged 18 to 44 years, with regular menstrual cycles. INTERVENTION(S): None. MAIN OUTCOME MEASURE(S): AMH and antral follicle count (AFC) determined at a single visit on day 2-4 of the menstrual cycle. RESULT(S): There was a statistically significant variance in AFC but not in AMH between centers. Both AFC and AMH varied by age (overall Spearman rho -0.50 for AFC and -0.47 for AMH), but there was also significant between-center variation in the relationship between AFC and age but not for AMH. There was a strong positive correlation between AMH and AFC (overall spearman rho 0.68), which varied from 0.49 to 0.87 between centers. An agreement table using AFC cutoffs of 7 and 15 showed classification agreement in 63.2%, 56.9% and 74.5% of women for low, medium, and high groups, respectively. CONCLUSION(S): The novel fully automated Elecsys AMH assay shows good correlations with age and AFC in women of reproductive age, providing a reproducible measure of the growing follicle pool.
Authors: Hang Wun Raymond Li; David Mark Robertson; Chris Burns; William Leigh Ledger Journal: Front Endocrinol (Lausanne) Date: 2021-05-24 Impact factor: 5.555
Authors: Kelly-Anne Phillips; Ian M Collins; Roger L Milne; Sue Anne McLachlan; Michael Friedlander; Martha Hickey; Catharyn Stern; John L Hopper; Richard Fisher; Gordon Kannemeyer; Sandra Picken; Charmaine D Smith; Thomas W Kelsey; Richard A Anderson Journal: Hum Reprod Date: 2016-05 Impact factor: 6.918