Anargyros Kouris1, Aikaterini Pistiki2, Alexandros Katoulis1, Marianna Georgitsi2, Sofia Giatrakou1, Evangelia Papadavid1, Mihai G Netea3, Nikolaos Stavrianeas1, Evangelos J Giamarellos-Bourboulis4. 1. 2nd Department of Dermatology and Venereology, National and Kapodistrian University of Athens, Medical School, Athens, Greece. 2. 4th Department of Internal Medicine, National and Kapodistrian University of Athens, Medical School, Athens, Greece. 3. Department of Medicine and Nijmegen Institute for Infection, Inflammation and Immunity, Radboud University Nijmegen Medical Centre, TheNetherlands. 4. 4th Department of Internal Medicine, National and Kapodistrian University of Athens, Medical School, Athens, Greece, Department of Medicine and Nijmegen Institute for Infection, Inflammation and Immunity, Radboud University Nijmegen Medical Centre, TheNetherlands.
Abstract
BACKGROUND: Psoriasis is one of the most common, immune-mediated, chronic inflammatory skin diseases. Proinflammatory cytokines play an important pathogenetic role at a local level. OBJECTIVE: To assess whether the proinflammatory cytokines IL-1β, IL-6, IL-17, IL-22 and TNF-α are released systemically during psoriasis. METHODS: Peripheral blood mononuclear cells (PBMCs) were isolated from 30 patients with psoriasis and 30 healthy volunteers. Cytokine production was assessed in supernatants using an enzyme immunoassay after stimulation of PBMCs with microbial stimuli. In addition, flow cytometry was used to determine the subsets of monocytes involved and the intracellular TNF-α production in monocytes. RESULTS: IL-17 levels were significantly higher in the supernatants of PBMCs from psoriatic patients after stimulation with phytohemagglutinin. TNF-α production was also significantly higher in cells from psoriatic patients after stimulation with all stimuli, as compared with health volunteers. Similar changes were not found for the other cytokines. A statistically significant difference was observed between patients and controls for inflammatory CD14(+)/CD16(+) monocytes (p<0.0001) and patrolling CD14-/CD16(+) monocytes. CONCLUSION: Hyper-production of TNF-α is documented in psoriasis. These results support the concept that there is a systemic, proinflammatory component in psoriasis.
BACKGROUND:Psoriasis is one of the most common, immune-mediated, chronic inflammatory skin diseases. Proinflammatory cytokines play an important pathogenetic role at a local level. OBJECTIVE: To assess whether the proinflammatory cytokines IL-1β, IL-6, IL-17, IL-22 and TNF-α are released systemically during psoriasis. METHODS: Peripheral blood mononuclear cells (PBMCs) were isolated from 30 patients with psoriasis and 30 healthy volunteers. Cytokine production was assessed in supernatants using an enzyme immunoassay after stimulation of PBMCs with microbial stimuli. In addition, flow cytometry was used to determine the subsets of monocytes involved and the intracellular TNF-α production in monocytes. RESULTS:IL-17 levels were significantly higher in the supernatants of PBMCs from psoriaticpatients after stimulation with phytohemagglutinin. TNF-α production was also significantly higher in cells from psoriaticpatients after stimulation with all stimuli, as compared with health volunteers. Similar changes were not found for the other cytokines. A statistically significant difference was observed between patients and controls for inflammatory CD14(+)/CD16(+) monocytes (p<0.0001) and patrolling CD14-/CD16(+) monocytes. CONCLUSION: Hyper-production of TNF-α is documented in psoriasis. These results support the concept that there is a systemic, proinflammatory component in psoriasis.
Authors: K Masek-Hammerman; E Peeva; A Ahmad; S Menon; M Afsharvand; R Peng Qu; J B Cheng; J Syed; Y Zhan; S P O'Neil; S Pleasic-Williams; L A Cox; D Beidler Journal: Clin Exp Immunol Date: 2015-11-09 Impact factor: 4.330
Authors: Anne T Kloek; Mercedes Valls Seron; Ben Schmand; Michael W T Tanck; Arie van der Ende; Matthijs C Brouwer; Diederik van de Beek Journal: Acta Neuropathol Commun Date: 2021-01-06 Impact factor: 7.801