| Literature DB >> 25678043 |
A Ševčovičová1, M Šemeláková2, J Plšíková3, D Loderer4, P Imreová4, E Gálová4, M Kožurková3, E Miadoková4, P Fedoročko2.
Abstract
The aim of this study was to explain the molecular mechanisms of action of hyperforin, a phluoroglucinol derivative found in Hypericum perforatum L. and its more stable derivative aristoforin. DNA-topology assay revealed partial DNA-protective activities of hyperforin and aristoforin against Fe(2+)-induced DNA breaks. In order to assess molecular mechanisms underlying DNA-protective activity, the potential antioxidant activity of hyperforin and aristoforin was investigated using DPPH and OH scavenging assays, reducing power assay and Fe(2+)-chelating assay. We also studied interaction of hyperforin and aristoforin with DNA using established protocols for fluorescence titration. The ability of the studied compounds to relax topoisomerase I with electrophoretic techniques was investigated. The reduction in the fluorescence of hyperforin indicated an interaction between hyperforin and DNA with a binding constant of 0.2×10(8)M(-1). We suggest that a mechanism of hyperforin/aristoforin DNA-protective abilities is based on free radicals (mainly OH) scavenging activity.Entities:
Keywords: Aristoforin; DNA unwinding assay; DNA-topology assay; DPPH()/()OH scavenging activity; Hyperforin; Reducing power assay
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Year: 2015 PMID： 25678043 DOI： 10.1016/j.tiv.2015.01.016
Source DB: PubMed Journal: Toxicol In Vitro ISSN： 0887-2333 Impact factor: 3.500