Zaher Merhi1, Alex J Polotsky2, Andrew P Bradford2, Erkan Buyuk3, Justin Chosich2, Tzu Phang2, Sangita Jindal3, Nanette Santoro2. 1. Division of Reproductive Biology, Department of Obstetrics and Gynecology, NYU Langone Medical Center, New York, NY, USA Division of Reproductive Endocrinology and Infertility, University of Vermont College of medicine, Burlington, VT, USA zom00@hotmail.com. 2. Division of Reproductive Endocrinology and Infertility, Department of Obstetrics and Gynecology, University of Colorado, Denver, CO, USA. 3. Division of Reproductive Endocrinology and Infertility, Department of Obstetrics and Gynecology and Women's Health, Albert Einstein College of Medicine, Bronx, NY, USA.
Abstract
OBJECTIVE: To determine whether obesity alters genes important in cellular growth and inflammation in human cumulus granulosa cells (GCs). METHODS: Eight reproductive-aged women who underwent controlled ovarian hyperstimulation followed by oocyte retrieval for in vitro fertilization were enrolled. Cumulus GC RNA was extracted and processed for microarray analysis on Affymetrix Human Genome U133 Plus 2.0 chips. Gene expression data were validated on GCs from additional biologically similar samples using quantitative real-time polymerase chain reaction (RT-PCR). Comparison in gene expression was made between women with body mass index (BMI) <25 kg/m(2) (group 1; n = 4) and those with BMI ≥25 kg/m(2) (group 2; n = 4). RESULTS: Groups 1 and 2 had significantly different BMI (21.4 ± 1.4 vs 30.4 ± 2.7 kg/m(2), respectively; P = .02) but did not differ in age (30.5 ± 1.7 vs 32.7 ± 0.3 years, respectively; P = .3). Comparative analysis of gene expression profiles by supervised clustering between group 1 versus group 2 resulted in the selection of 7 differentially expressed genes: fibroblast growth factor 12 (FGF-12), protein phosphatase 1-like (PPM1L), zinc finger protein multitype 2 (ZFPM2), forkhead box M1 (FOXM1), cell division cycle 20 (CDC20), interleukin 1 receptor-like 1 (IL1RL1), and growth arrest-specific protein 7 (GAS7). FOXM1, CDC20, and GAS7 were downregulated while FGF-12 and PPM1L were upregulated in group 2 when compared to group 1. Validation with RT-PCR confirmed the microarray data except for ZFPM2 and IL1RL. As BMI increased, expression of FOXM1 significantly decreased (r = -.60, P = .048). CONCLUSIONS: Adiposity is associated with changes in the expression of genes important in cellular growth, cell cycle progression, and inflammation. The upregulation of the metabolic regulator gene PPM1L suggests that adiposity induces an abnormal metabolic follicular environment, potentially altering folliculogenesis and oocyte quality.
OBJECTIVE: To determine whether obesity alters genes important in cellular growth and inflammation in human cumulus granulosa cells (GCs). METHODS: Eight reproductive-aged women who underwent controlled ovarian hyperstimulation followed by oocyte retrieval for in vitro fertilization were enrolled. Cumulus GC RNA was extracted and processed for microarray analysis on Affymetrix Human Genome U133 Plus 2.0 chips. Gene expression data were validated on GCs from additional biologically similar samples using quantitative real-time polymerase chain reaction (RT-PCR). Comparison in gene expression was made between women with body mass index (BMI) <25 kg/m(2) (group 1; n = 4) and those with BMI ≥25 kg/m(2) (group 2; n = 4). RESULTS: Groups 1 and 2 had significantly different BMI (21.4 ± 1.4 vs 30.4 ± 2.7 kg/m(2), respectively; P = .02) but did not differ in age (30.5 ± 1.7 vs 32.7 ± 0.3 years, respectively; P = .3). Comparative analysis of gene expression profiles by supervised clustering between group 1 versus group 2 resulted in the selection of 7 differentially expressed genes: fibroblast growth factor 12 (FGF-12), protein phosphatase 1-like (PPM1L), zinc finger protein multitype 2 (ZFPM2), forkhead box M1 (FOXM1), cell division cycle 20 (CDC20), interleukin 1 receptor-like 1 (IL1RL1), and growth arrest-specific protein 7 (GAS7). FOXM1, CDC20, and GAS7 were downregulated while FGF-12 and PPM1L were upregulated in group 2 when compared to group 1. Validation with RT-PCR confirmed the microarray data except for ZFPM2 and IL1RL. As BMI increased, expression of FOXM1 significantly decreased (r = -.60, P = .048). CONCLUSIONS: Adiposity is associated with changes in the expression of genes important in cellular growth, cell cycle progression, and inflammation. The upregulation of the metabolic regulator gene PPM1L suggests that adiposity induces an abnormal metabolic follicular environment, potentially altering folliculogenesis and oocyte quality.
Authors: Eun Ji Kim; Hea Young Oh; Hyoung-Sam Heo; Ji Eun Hong; Sung-Jae Jung; Ki Won Lee; Jong Hoon Park; Cheol-Goo Hur; Jung Han Yoon Park Journal: Br J Nutr Date: 2012-12-13 Impact factor: 3.718
Authors: José Bellver; José Antonio Martínez-Conejero; Elena Labarta; Pilar Alamá; Marco Antonio Barreto Melo; José Remohí; Antonio Pellicer; José Antonio Horcajadas Journal: Fertil Steril Date: 2011-04-09 Impact factor: 7.329
Authors: Fang Jin; Masakazu Hamada; Liviu Malureanu; Karthik B Jeganathan; Wei Zhou; Dean E Morbeck; Jan M van Deursen Journal: PLoS Genet Date: 2010-09-30 Impact factor: 5.917
Authors: Allison A Hedley; Cynthia L Ogden; Clifford L Johnson; Margaret D Carroll; Lester R Curtin; Katherine M Flegal Journal: JAMA Date: 2004-06-16 Impact factor: 56.272
Authors: José Bellver; Marco A B Melo; Ernesto Bosch; Vicente Serra; José Remohí; Antonio Pellicer Journal: Fertil Steril Date: 2007-04-06 Impact factor: 7.329
Authors: Meghan L Ruebel; Matthew Cotter; Clark R Sims; Dean M Moutos; Thomas M Badger; Mario A Cleves; Kartik Shankar; Aline Andres Journal: J Clin Endocrinol Metab Date: 2017-06-01 Impact factor: 5.958
Authors: G M Yerushalmi; M Salmon-Divon; L Ophir; Y Yung; M Baum; G Coticchio; R Fadini; M Mignini-Renzini; M Dal Canto; R Machtinger; E Maman; A Hourvitz Journal: Sci Rep Date: 2018-10-23 Impact factor: 4.379