Peggy Kloppot1, Martina Selle2, Christian Kohler1, Sebastian Stentzel3, Stephan Fuchs1, Volkmar Liebscher4, Elke Müller5, Devika Kale6, Knut Ohlsen2, Barbara M Bröker3, Peter F Zipfel7,8, Barbara C Kahl6, Ralf Ehricht5, Michael Hecker1, Susanne Engelmann1,9,10. 1. Institut für Mikrobiologie, Universität Greifswald, Greifswald, Germany. 2. Institut für Molekulare Infektionsbiologie, Universität Würzburg, Würzburg, Germany. 3. Institut für Immunologie und Transfusionsmedizin, Universitätsmedizin Greifswald, Greifswald, Germany. 4. Institut für Mathematik und Informatik, Universität Greifswald, Greifswald, Germany. 5. Alere Technologies GmbH Jena, Jena, Germany. 6. Institut für Medizinische Mikrobiologie, Universitätsklinikum Münster, Münster, Germany. 7. Infektionsbiologie, Hans-Knöll-Institut Jena, Münster, Germany. 8. Friedrich Schiller Universität, Jena, Germany. 9. Institut für Mikrobiologie, TU Braunschweig, Braunschweig, Germany. 10. Mikrobielle Proteomik, Helmholtzzentrum für Infektionsforschung, Braunschweig, Germany.
Abstract
PURPOSE: The mortality rate of patients with Staphylococcus aureus infections is alarming and urgently demands new strategies to attenuate the course of these infections or to detect them at earlier stages. EXPERIMENTAL DESIGN: To study the adaptive immune response to S. aureus antigens in healthy human volunteers, a protein microarray containing 44 S. aureus proteins was developed using the ArrayStrip platform technology. RESULTS: Testing plasma samples from 15 S. aureus carriers and 15 noncarriers 21 immunogenic S. aureus antigens have been identified. Seven antigens were recognized by antibodies present in at least 60% of the samples, representing the core S. aureus immunome of healthy individuals. S. aureus-specific serum immunoglobulin G (IgG) levels were significantly lower in noncarriers than in carriers specifically anti-IsaA, anti-SACOL0479, and anti-SACOL0480 IgGs were found at lower frequencies and quantities. Twenty-two antigens present on the microarray were encoded by all S. aureus carrier isolates. Nevertheless, the immune system of the carriers was responsive to only eight of them and with different intensities. CONCLUSION AND CLINICAL RELEVANCE: The established protein microarray allows a broad profiling of the S. aureus-specific antibody response and can be used to identify S. aureus antigens that might serve as vaccines or diagnostic markers.
PURPOSE: The mortality rate of patients with Staphylococcus aureus infections is alarming and urgently demands new strategies to attenuate the course of these infections or to detect them at earlier stages. EXPERIMENTAL DESIGN: To study the adaptive immune response to S. aureus antigens in healthy human volunteers, a protein microarray containing 44 S. aureus proteins was developed using the ArrayStrip platform technology. RESULTS: Testing plasma samples from 15 S. aureus carriers and 15 noncarriers 21 immunogenic S. aureus antigens have been identified. Seven antigens were recognized by antibodies present in at least 60% of the samples, representing the core S. aureus immunome of healthy individuals. S. aureus-specific serum immunoglobulin G (IgG) levels were significantly lower in noncarriers than in carriers specifically anti-IsaA, anti-SACOL0479, and anti-SACOL0480 IgGs were found at lower frequencies and quantities. Twenty-two antigens present on the microarray were encoded by all S. aureus carrier isolates. Nevertheless, the immune system of the carriers was responsive to only eight of them and with different intensities. CONCLUSION AND CLINICAL RELEVANCE: The established protein microarray allows a broad profiling of the S. aureus-specific antibody response and can be used to identify S. aureus antigens that might serve as vaccines or diagnostic markers.
Authors: Dennis G A M Koedijk; Francisco Romero Pastrana; Hedzer Hoekstra; Sanne van den Berg; Jaap Willem Back; Carolien Kerstholt; Rianne C Prins; Irma A J M Bakker-Woudenberg; Jan Maarten van Dijl; Girbe Buist Journal: Sci Rep Date: 2017-08-15 Impact factor: 4.379
Authors: Silva Holtfreter; Julia Kolata; Sebastian Stentzel; Stephanie Bauerfeind; Frank Schmidt; Nandakumar Sundaramoorthy; Barbara M Bröker Journal: Proteomes Date: 2016-03-07
Authors: Michael Widjaja; Kate Louise Harvey; Lisa Hagemann; Iain James Berry; Veronica Maria Jarocki; Benjamin Bernard Armando Raymond; Jessica Leigh Tacchi; Anne Gründel; Joel Ricky Steele; Matthew Paul Padula; Ian George Charles; Roger Dumke; Steven Philip Djordjevic Journal: Sci Rep Date: 2017-09-11 Impact factor: 4.379