Zhuoyi Zhang1, Limin Ding1, Liqun Wu2, Liying Xu1, Lanzhi Zheng1, Xiaomin Huang1. 1. Department of Emergency, The First Affiliated Hospital of Zhejiang Chinese Medical University Hangzhou 310006, Zhejiang Province, China. 2. Department of Emergency, Taizhou Central Hospital Taizhou 318000, Zhejiang Province, China.
Abstract
OBJECTIVE: This study was designed to investigate the protective effects of salidroside (SDS) via suppressing the expression of transforming growth factor-β1 (TGF-β1) in rat acute lung injury (ALI) induced by paraquat (PQ) and to explore the potential molecular mechanisms. METHODS: A total of 90 male rats (190-210 g) were randomly and evenly divided into 9 groups: control group, PQ groups (4 groups), and PQ + SDS groups (4 groups). The rats in control group were treated with equal volume of saline intraperitoneally. The rats in PQ groups were exposed to PQ solution (20 mg/kg) by gastric gavage for 1, 6, 24, and 72 hours, respectively. The rats in PQ + SDS groups were intraperitoneally injected once with SDS (10 mg/kg) every 12 hours after PQ perfusion. Pulmonary pathological changes were observed by hematoxylin and eosin (HE) staining. The expression of TGF-β1 and the mRNA were evaluated by immunohistochemical (IHC) scoring and real time quantitative reverse transcription polymerase chain reaction (real-time qRT-PCR), respectively. RESULTS: SDS alleviated the symptoms of PQ induced ALI. Moreover, SDS reduced the expression of the inflammatory cytokine TGF-β1 including TGF-β1 IHC scores (at each time point from 6 to 72 hours after PQ perfusion) and mRNA level (at each time point from 1 to 72 hours after PQ perfusion) compared with PQ groups (P < 0.05). CONCLUSION: SDS alleviated the pulmonary symptoms of PQ-induced ALI, at least partially, by repressing inflammatory cell infiltration and the expression of TGF-β1 resulting in delayed lung fibrosis.
OBJECTIVE: This study was designed to investigate the protective effects of salidroside (SDS) via suppressing the expression of transforming growth factor-β1 (TGF-β1) in ratacute lung injury (ALI) induced by paraquat (PQ) and to explore the potential molecular mechanisms. METHODS: A total of 90 male rats (190-210 g) were randomly and evenly divided into 9 groups: control group, PQ groups (4 groups), and PQ + SDS groups (4 groups). The rats in control group were treated with equal volume of saline intraperitoneally. The rats in PQ groups were exposed to PQ solution (20 mg/kg) by gastric gavage for 1, 6, 24, and 72 hours, respectively. The rats in PQ + SDS groups were intraperitoneally injected once with SDS (10 mg/kg) every 12 hours after PQ perfusion. Pulmonary pathological changes were observed by hematoxylin and eosin (HE) staining. The expression of TGF-β1 and the mRNA were evaluated by immunohistochemical (IHC) scoring and real time quantitative reverse transcription polymerase chain reaction (real-time qRT-PCR), respectively. RESULTS:SDS alleviated the symptoms of PQ induced ALI. Moreover, SDS reduced the expression of the inflammatory cytokine TGF-β1 including TGF-β1 IHC scores (at each time point from 6 to 72 hours after PQ perfusion) and mRNA level (at each time point from 1 to 72 hours after PQ perfusion) compared with PQ groups (P < 0.05). CONCLUSION:SDS alleviated the pulmonary symptoms of PQ-induced ALI, at least partially, by repressing inflammatory cell infiltration and the expression of TGF-β1 resulting in delayed lung fibrosis.
Authors: Rômulo D Novaes; Reggiani V Gonçalves; Marli C Cupertino; Daiane C S Marques; Damiana D Rosa; Maria do Carmo G Peluzio; Clóvis A Neves; João Paulo V Leite Journal: Int J Exp Pathol Date: 2012-03-19 Impact factor: 1.925
Authors: Patricia R M Rocco; Alba B Souza; Debora S Faffe; Caroline P Pássaro; Flávia B Santos; Elnara M Negri; Januário G M Lima; Renata S Contador; Vera L Capelozzi; Walter A Zin Journal: Am J Respir Crit Care Med Date: 2003-07-03 Impact factor: 21.405
Authors: Cunfang Qi; Junfeng Zhang; Xinlin Chen; JiansHui Zhang; Pengbo Yang; Qian Jiao; Pengbo Zhang; Hai-Xia Lu; Yong Liu Journal: Nan Fang Yi Ke Da Xue Xue Bao Date: 2013-07