Haiyan Liu1, Xudong Yang2, Wei Hou1. 1. Department of Pediatrics, The Second Affiliated Hospital of Xi'an Jiaotong University Xi'an 710004, China. 2. Department of Biochemistry and Molecular Biology, Medical School of Xi'an Jiaotong University Xi'an 710061, China.
Abstract
OBJECTIVE: To investigate and analyze the correlation between the c-fos protein expression and neuropeptide content in the lung of bronchial asthmatic rats. METHODS: Thirty-two (32) SD rats were randomly allocated into 4 groups of the normal control, the non-acute asthma, the acute asthma and the dexamethasone intervention. Immunohistochemistry was performed for histological observation, and substance P (SP) and vasoactive intestinal peptide (VIP) concentrations in the bronchoalveolar lavage fluid were measured by enzyme-linked immunosorbent assay (ELISA). RESULTS: SP concentration in the alveolar lavage of asthmatic rat was significantly higher than that in the normal control group (P < 0.0001), whereas VIP concentration was significantly lower (P < 0.0001). The optical density of c-fos protein in the lung tissues of groups of the non-acute asthma, the acute asthma and the dexamethasone intervention was positively correlated with SP concentration in the bronchoalveolar lavage fluid (r = 0.908, r = 0.967, r = 0.865), and negatively correlated with the VIP concentration in the alveolar lavage (r = -0.974, r = -0.949, r = -0.962). CONCLUSION: The c-fos protein expression and neuropeptide content in the lungs of asthmatic rats are related with asthma attacks.
OBJECTIVE: To investigate and analyze the correlation between the c-fos protein expression and neuropeptide content in the lung of bronchial asthmatic rats. METHODS: Thirty-two (32) SD rats were randomly allocated into 4 groups of the normal control, the non-acute asthma, the acute asthma and the dexamethasone intervention. Immunohistochemistry was performed for histological observation, and substance P (SP) and vasoactive intestinal peptide (VIP) concentrations in the bronchoalveolar lavage fluid were measured by enzyme-linked immunosorbent assay (ELISA). RESULTS: SP concentration in the alveolar lavage of asthmatic rat was significantly higher than that in the normal control group (P < 0.0001), whereas VIP concentration was significantly lower (P < 0.0001). The optical density of c-fos protein in the lung tissues of groups of the non-acute asthma, the acute asthma and the dexamethasone intervention was positively correlated with SP concentration in the bronchoalveolar lavage fluid (r = 0.908, r = 0.967, r = 0.865), and negatively correlated with the VIP concentration in the alveolar lavage (r = -0.974, r = -0.949, r = -0.962). CONCLUSION: The c-fos protein expression and neuropeptide content in the lungs of asthmatic rats are related with asthma attacks.
Authors: Elke Hoffmann; Axel Thiefes; Daniela Buhrow; Oliver Dittrich-Breiholz; Heike Schneider; Klaus Resch; Michael Kracht Journal: J Biol Chem Date: 2004-12-21 Impact factor: 5.157