Noemí Arias1,2, M Teresa Macarulla1,2, Leixuri Aguirre1,2, Iñaki Milton1, María P Portillo3,4. 1. Nutrition and Obesity Group, Department of Nutrition and Food Science, Faculty of Pharmacy and Lucio Lascaray Research Center, University of the Basque Country (UPV/EHU), Paseo de la Universidad 7, 01006, Vitoria, Spain. 2. CIBER de Fisiopatología de la Obesidad y Nutrición (CIBERobn), Instituto de Salud Carlos III, Vitoria, Spain. 3. Nutrition and Obesity Group, Department of Nutrition and Food Science, Faculty of Pharmacy and Lucio Lascaray Research Center, University of the Basque Country (UPV/EHU), Paseo de la Universidad 7, 01006, Vitoria, Spain. mariapuy.portillo@ehu.es. 4. CIBER de Fisiopatología de la Obesidad y Nutrición (CIBERobn), Instituto de Salud Carlos III, Vitoria, Spain. mariapuy.portillo@ehu.es.
Abstract
PURPOSE: The aim of this study was to analyze whether the combination of resveratrol and quercetin showed additive or synergic effects on body fat accumulation and triacylglycerol metabolism in adipose tissue from rats fed an obesogenic diet. METHODS: Rats were divided into four dietary groups: a control group and three groups each treated with either resveratrol (15 mg/kg/day; RSV), quercetin (30 mg/kg/day; Q), or both (15 mg resveratrol/kg/day and 30 mg quercetin/kg/day; RSV + Q) for 6 weeks. White adipose tissues from several anatomical locations were dissected. Serum parameters were analyzed by using commercial kits. The activities of fatty acid synthase and heparin-releasable lipoprotein lipase (HR-LPL) were measured using spectrophotometric and fluorimetric methods, and protein expression of acetyl-CoA carboxylase (ACC), adipose tissue triglyceride lipase (ATGL), and hormone-sensitive lipase (HSL) by western blot. RESULTS: The administration of either resveratrol or quercetin separately did not induce significant reductions in adipose tissue weights. By contrast, the combination of both molecules led to a significant reduction in all the fat depots analyzed. The percentage of reduction in each tissue was greater than the calculated additive effect. HR-LPL activity was reduced in RSV and RSV + Q groups. The activity of HSL was not modified. By contrast, ACC was inhibited and ATGL increased only by the combination of both polyphenols. CONCLUSION: The results obtained demonstrate a synergistic effect between resveratrol and quercetin and suggest that when these molecules are combined, a great number of metabolic pathways involved in adipose tissue triacylglycerol accumulation are affected.
PURPOSE: The aim of this study was to analyze whether the combination of resveratrol and quercetin showed additive or synergic effects on body fat accumulation and triacylglycerol metabolism in adipose tissue from rats fed an obesogenic diet. METHODS:Rats were divided into four dietary groups: a control group and three groups each treated with either resveratrol (15 mg/kg/day; RSV), quercetin (30 mg/kg/day; Q), or both (15 mg resveratrol/kg/day and 30 mg quercetin/kg/day; RSV + Q) for 6 weeks. White adipose tissues from several anatomical locations were dissected. Serum parameters were analyzed by using commercial kits. The activities of fatty acid synthase and heparin-releasable lipoprotein lipase (HR-LPL) were measured using spectrophotometric and fluorimetric methods, and protein expression of acetyl-CoA carboxylase (ACC), adipose tissue triglyceride lipase (ATGL), and hormone-sensitive lipase (HSL) by western blot. RESULTS: The administration of either resveratrol or quercetin separately did not induce significant reductions in adipose tissue weights. By contrast, the combination of both molecules led to a significant reduction in all the fat depots analyzed. The percentage of reduction in each tissue was greater than the calculated additive effect. HR-LPL activity was reduced in RSV and RSV + Q groups. The activity of HSL was not modified. By contrast, ACC was inhibited and ATGL increased only by the combination of both polyphenols. CONCLUSION: The results obtained demonstrate a synergistic effect between resveratrol and quercetin and suggest that when these molecules are combined, a great number of metabolic pathways involved in adipose tissue triacylglycerol accumulation are affected.
Entities:
Keywords:
Obesity; Quercetin; Rat; Resveratrol; White adipose tissue
Authors: N Arias; J Miranda; M T Macarulla; L Aguirre; A Fernández-Quintela; C Andres-Lacueva; M Urpi-Sarda; M P Portillo Journal: Eur J Nutr Date: 2013-07-30 Impact factor: 5.614
Authors: Jun Yoshino; Caterina Conte; Luigi Fontana; Bettina Mittendorfer; Shin-ichiro Imai; Kenneth B Schechtman; Charles Gu; Iris Kunz; Filippo Rossi Fanelli; Bruce W Patterson; Samuel Klein Journal: Cell Metab Date: 2012-10-25 Impact factor: 27.287
Authors: C Carpéné; S Gomez-Zorita; R Gupta; S Grès; C Rancoule; T Cadoudal; J Mercader; A Gomez; C Bertrand; Z Iffiu-Soltész Journal: Eur J Nutr Date: 2014-02-15 Impact factor: 5.614
Authors: Alfredo Fernández-Quintela; Christian Carpéné; Maialen Fernández; Leixuri Aguirre; Iñaki Milton-Laskibar; José Contreras; Maria P Portillo Journal: J Physiol Biochem Date: 2016-12-15 Impact factor: 4.158
Authors: Ana Elena Peredo-Escárcega; Verónica Guarner-Lans; Israel Pérez-Torres; Sergio Ortega-Ocampo; Elizabeth Carreón-Torres; Vicente Castrejón-Tellez; Eulises Díaz-Díaz; María Esther Rubio-Ruiz Journal: Evid Based Complement Alternat Med Date: 2015-11-01 Impact factor: 2.629
Authors: Reilly T Enos; Kandy T Velázquez; Meredith S Carson; Jamie L McClellan; Prakash Nagarkatti; Mitzi Nagarkatti; J Mark Davis; E Angela Murphy Journal: PLoS One Date: 2016-12-13 Impact factor: 3.240