Chune Li1, Jinshui Yang1, Xin Wang1, Entao Wang2, Baozhen Li1, Ruoxue He1, Hongli Yuan3. 1. State Key Laboratories for Agrobiotechnology, College of Biological Sciences, China Agricultural University, Beijing, People's Republic of China. 2. Departamento de Microbiología, Escuela Nacional de Ciencias Biológicas, Instituto Politécnico Nacional, Mexico, DF, Mexico. 3. State Key Laboratories for Agrobiotechnology, College of Biological Sciences, China Agricultural University, Beijing, People's Republic of China. Electronic address: hlyuan@cau.edu.cn.
Abstract
Phosphate accumulating bacterium Pseudomonas stutzeri YG-24 exhibited efficient heterotrophic nitrification and aerobic denitrification ability. Single factor experiments showed that both heterotrophic nitrification and aerobic denitrification occurred with sodium citrate as carbon source and lower C/N ratio of 8. High average NH4(+)-N, NO2(-)-N and NO3(-)-N removal rates of 8.75, 7.51 and 7.73 mg L(-1)h(-1) were achieved. The application of strain YG-24 in wastewater samples resulted in TN, NH4(+)-N, NO2(-)-N, NO3(-)-N and P removal efficiencies of 85.28%, 88.13%, 86.15%, 70.83% and 51.21%. Sequencing and quantitative amplification by real-time PCR of napA, nirS and ppk showed that nitrogen removal pathway of strain YG-24 was achieved through heterotrophic ammonium nitrification coupled with fast nitrite denitrification (NH4(+)-N to NO2(-)-N and then to gaseous nitrogen) directly. These results demonstrated the strain as a suitable candidate to simultaneously remove both nitrogen and phosphate in wastewater treatment.
Phosphate accumulating bacterium n class="Species">Pseudomonas stutzeri YG-24 exhibited efficient heterotrophic nitrification and aerobic denitrification ability. Single factor experiments showed that both heterotrophic nitrification and aerobic denitrification occurred with sodium citrate as carbon source and lower C/N ratio of 8. High average NH4(+)-N, NO2(-)-N and NO3(-)-N removal rates of 8.75, 7.51 and 7.73 mg L(-1)h(-1) were achieved. The application of strain YG-24 in wastewater samples resulted in TN, NH4(+)-N, NO2(-)-N, NO3(-)-N and P removal efficiencies of 85.28%, 88.13%, 86.15%, 70.83% and 51.21%. Sequencing and quantitative amplification by real-time PCR of napA, nirS and ppk showed that nitrogen removal pathway of strain YG-24 was achieved through heterotrophic ammonium nitrification coupled with fast nitrite denitrification (NH4(+)-N to NO2(-)-N and then to gaseous nitrogen) directly. These results demonstrated the strain as a suitable candidate to simultaneously remove both nitrogen and phosphate in wastewater treatment.
Authors: Giovanni Nicoletti; Marco Saler; Tommaso Pellegatta; Marco Mario Tresoldi; Viola Bonfanti; Alberto Malovini; Angela Faga; Federica Riva Journal: Biomed Rep Date: 2017-10-18
Authors: Xiaoying Zheng; Mengqi Jin; Hang Xu; Wei Chen; Yuan Zhang; Mengmeng Yang; Xiaoyao Shao; Zhi Xu; Weihong Wang Journal: Int J Environ Res Public Health Date: 2019-07-24 Impact factor: 3.390