Marc Thill1, Alena Woeste2, Kathrin Reichert2, Dorothea Fischer2, Achim Rody2, Michael Friedrich3, Frank Köster4. 1. Department of Gynecology and Obstetrics, Agaplesion Markus Hospital, Frankfurt am Main, Germany. 2. Department of Gynecology and Obstetrics, University of Lübeck, Lübeck, Germany. 3. Department of Gynecology and Obstetrics, Helios Hospital Krefeld, Krefeld, Germany. 4. Department of Gynecology and Obstetrics, University of Lübeck, Lübeck, Germany frank.koester@uksh.de.
Abstract
BACKGROUND: Vitamin D exhibits multiple anti-proliferative and pro-differentiating actions. Prostaglandin-(PG)E2 is a tumor-promoting tissue hormone anabolized by cyclooxygenase-2 (COX-2). Recently, a link between the PG and vitamin D metabolism was reported. MATERIALS AND METHODS: The influence of calcitriol and celecoxib on the proliferation of ovarian cancer cell lines was measured and the impact of calcitriol on the protein and mRNA expression of COX-2 was quantified by western blot and qRT-PCR, respectively. RESULTS: After COX-2 induction with interleukin (IL)-1β, 10 μM celecoxib did not significantly inhibit the proliferation of OVCAR-3 cells, whereas calcitriol showed such an effect; however, the combination of the two substances had an additive influence. After induction by IL-1β, calcitriol inhibited the COX-2 protein, as well as its mRNA expression significantly in OVCAR-3 and SKOV-3 cells. CONCLUSION: These data suggest a correlation between PG and vitamin D metabolism in their anti-tumorigenic activity in ovarian carcinomas. Copyright
BACKGROUND:Vitamin D exhibits multiple anti-proliferative and pro-differentiating actions. Prostaglandin-(PG)E2 is a tumor-promoting tissue hormone anabolized by cyclooxygenase-2 (COX-2). Recently, a link between the PG and vitamin D metabolism was reported. MATERIALS AND METHODS: The influence of calcitriol and celecoxib on the proliferation of ovarian cancer cell lines was measured and the impact of calcitriol on the protein and mRNA expression of COX-2 was quantified by western blot and qRT-PCR, respectively. RESULTS: After COX-2 induction with interleukin (IL)-1β, 10 μM celecoxib did not significantly inhibit the proliferation of OVCAR-3 cells, whereas calcitriol showed such an effect; however, the combination of the two substances had an additive influence. After induction by IL-1β, calcitriol inhibited the COX-2 protein, as well as its mRNA expression significantly in OVCAR-3 and SKOV-3 cells. CONCLUSION: These data suggest a correlation between PG and vitamin D metabolism in their anti-tumorigenic activity in ovarian carcinomas. Copyright
Authors: Anna A Brożyna; Tae-Kang Kim; Marzena Zabłocka; Wojciech Jóźwicki; Junming Yue; Robert C Tuckey; Anton M Jetten; Andrzej T Slominski Journal: Nutrients Date: 2020-11-19 Impact factor: 5.717