Marc Thill1, Kathrin Reichert2, Alena Woeste2, Stephan Polack2, Dorothea Fischer2, Friederike Hoellen2, Achim Rody2, Michael Friedrich3, Frank Köster4. 1. Department of Gynecology and Obstetrics, Agaplesion Markus Hospital, Frankfurt am Main, Germany. 2. Department of Gynecology and Obstetrics, University of Lübeck, Lübeck, Germany. 3. Department of Gynecology and Obstetrics, Helios Hospital Krefeld, Krefeld, Germany. 4. Department of Gynecology and Obstetrics, University of Lübeck, Lübeck, Germany frank.koester@uksh.de.
Abstract
BACKGROUND: Vitamin D is known for its anti-cancerogenous potential. Prostaglandin E2 (PGE2) is a proliferation and inflammation activating agent. The production of PGE2 is dependent on the activity of cyclooxygenase-2 (COX-2). A link between vitamin D and PGE2 metabolism was recently shown. MATERIALS AND METHODS: In MDA-MB-231 and MCF-7 breast cancer cell lines we investigated the influence of calcitriol and the COX-2 inhibitor celecoxib regarding cell growth via MTT test, as well as on the protein and mRNA expression of COX-2 using western blot and qRT-PCR. RESULTS: The proliferation of MCF-7 and MDA-MB-231 was inhibited by both calcitriol and the COX-2 inhibitor celecoxib and even stronger by their combination. Moreover, calcitriol inhibited the COX-2 protein expression in MDA-MB-231, as well as the COX-2 mRNA expression in both cell lines. CONCLUSION: The combination of calcitriol and celecoxib demonstrated a cooperative growth-inhibiting effect in breast cancer cell lines. Copyright
BACKGROUND:Vitamin D is known for its anti-cancerogenous potential. Prostaglandin E2 (PGE2) is a proliferation and inflammation activating agent. The production of PGE2 is dependent on the activity of cyclooxygenase-2 (COX-2). A link between vitamin D and PGE2 metabolism was recently shown. MATERIALS AND METHODS: In MDA-MB-231 and MCF-7 breast cancer cell lines we investigated the influence of calcitriol and the COX-2 inhibitor celecoxib regarding cell growth via MTT test, as well as on the protein and mRNA expression of COX-2 using western blot and qRT-PCR. RESULTS: The proliferation of MCF-7 and MDA-MB-231 was inhibited by both calcitriol and the COX-2 inhibitor celecoxib and even stronger by their combination. Moreover, calcitriol inhibited the COX-2 protein expression in MDA-MB-231, as well as the COX-2 mRNA expression in both cell lines. CONCLUSION: The combination of calcitriol and celecoxib demonstrated a cooperative growth-inhibiting effect in breast cancer cell lines. Copyright
Authors: Zongtao Lin; Srinivasa Reddy Marepally; Dejian Ma; Linda K Myers; Arnold E Postlethwaite; Robert C Tuckey; Chloe Y S Cheng; Tae-Kang Kim; Junming Yue; Andrzej T Slominski; Duane D Miller; Wei Li Journal: J Med Chem Date: 2015-09-28 Impact factor: 7.446