Maria Vernet-Tomas1, Marisa Mena2, Laia Alemany3, Ignacio Bravo2, Silvia De Sanjosé3, Pau Nicolau4, Alba Bergueiro4, Josep Maria Corominas5, Sergi Serrano6, Ramon Carreras7, Belen Lloveras8. 1. Obstetrics and Gynaecology Department, Hospital del Mar, Barcelona, Spain Breast Cancer Unit, Hospital del Mar, Barcelona, Spain Department of Paediatrics, Obstetrics and Gynaecology and Preventive Medicine, Autonomous University of Barcelona, Barcelona, Spain mvernet@hospitaldelmar.cat. 2. Unit of Infections and Cancer, Cancer Epidemiology and Research Program, IDIBELL, Catalan Institute of Oncology, Hospitalet de Llobregat, Spain. 3. Unit of Infections and Cancer, Cancer Epidemiology and Research Program, IDIBELL, Catalan Institute of Oncology, Hospitalet de Llobregat, Spain CIBER Epidemiology and Public Health, Barcelona, Spain. 4. Obstetrics and Gynaecology Department, Hospital del Mar, Barcelona, Spain. 5. Breast Cancer Unit, Hospital del Mar, Barcelona, Spain Department of Paediatrics, Obstetrics and Gynaecology and Preventive Medicine, Autonomous University of Barcelona, Barcelona, Spain Pathology Department, Hospital del Mar, Barcelona, Spain. 6. Department of Paediatrics, Obstetrics and Gynaecology and Preventive Medicine, Autonomous University of Barcelona, Barcelona, Spain Pathology Department, Hospital del Mar, Barcelona, Spain. 7. Obstetrics and Gynaecology Department, Hospital del Mar, Barcelona, Spain Department of Paediatrics, Obstetrics and Gynaecology and Preventive Medicine, Autonomous University of Barcelona, Barcelona, Spain. 8. Pathology Department, Hospital del Mar, Barcelona, Spain.
Abstract
BACKGROUND/AIM: Great controversy exists about the association between Human Papillomavirus (HPV) and breast tumors. The aim of this study was to explore the presence of HPV DNA in a large set of breast cancer cases. MATERIALS AND METHODS: Techniques used followed the standards for an international retrospective survey of HPV-DNA genotyping, coordinated by our own group and the DDL Laboratories in Rijswijk, the Netherlands. Paraffin-embedded samples were used. SPF-10 broad-spectrum primers were applied, followed by deoxyribonucleic acid enzyme immunoassay and genotyping by reverse-line probe assay. RESULTS: A total of 78 samples were included in the study, 2 of benign conditions and 76 carcinomas, including different histological subtypes. HPV was not present in any of the specimens studied irrespective of histology, hormonal status and stage of disease. CONCLUSION: Our data do not support the involvement of HPV in breast carcinogenesis as no evidence of its presence was found. Copyright
BACKGROUND/AIM: Great controversy exists about the association between Human Papillomavirus (HPV) and breast tumors. The aim of this study was to explore the presence of HPV DNA in a large set of breast cancer cases. MATERIALS AND METHODS: Techniques used followed the standards for an international retrospective survey of HPV-DNA genotyping, coordinated by our own group and the DDL Laboratories in Rijswijk, the Netherlands. Paraffin-embedded samples were used. SPF-10 broad-spectrum primers were applied, followed by deoxyribonucleic acid enzyme immunoassay and genotyping by reverse-line probe assay. RESULTS: A total of 78 samples were included in the study, 2 of benign conditions and 76 carcinomas, including different histological subtypes. HPV was not present in any of the specimens studied irrespective of histology, hormonal status and stage of disease. CONCLUSION: Our data do not support the involvement of HPV in breast carcinogenesis as no evidence of its presence was found. Copyright
Authors: Abdallah Mohammed Elagali; Ahmed Abdelbadie Suliman; Mohammed Altayeb; Anas Ibrahim Dannoun; Narasimha Reddy Parine; Hader Ibrahim Sakr; Howayda Saeed Suliman; Moustafa Elsaeid Motawee Journal: Pan Afr Med J Date: 2021-01-15
Authors: Orla M Gannon; Annika Antonsson; Michael Milevskiy; Melissa A Brown; Nicholas A Saunders; Ian C Bennett Journal: Sci Rep Date: 2015-12-14 Impact factor: 4.379