PURPOSE: Bone-tendon healing following anterior cruciate ligament reconstruction is reportedly enhanced by bone morphogenetic protein (BMP)-7. To improve our understanding of the underlying biologic processes, we examined the effects of BMP-7 on region-specific gene expression in vitro. METHODS: A murine in vitro co-culture model simulating the osteoblast, interface and fibroblast regions was established. The dose- and time-dependent region-specific effects of BMP-7 exposure on gene expression of Alpl, Bglap, Col1a1, Runx2 and Spp1 were analysed by quantitative PCR. RESULTS: At the osteoblast region, BMP-7 significantly increased Alp, Bglap, Col1a1, and Runx2 expression, while Spp1 expression was suppressed. At the interface region, BMP-7 exposure resulted in a trend towards increased expression rates of Alpl and Col1a1, whereas Bglap (P < 0.001) and Runx2 (P < 0.01) were significantly upregulated without any detectable effect on Spp1 expression. At the fibroblast region, BMP-7 increased Alpl (P < 0.001), Bglap (P < 0.001) and Runx2 (P < 0.001) expression, but no significant effects were seen on Col1a1 or Spp1. Exposure to BMP-7 (100 ng/ml) had its most pronounced biologic impact on day ten. CONCLUSION: BMP-7 stimulation showed beneficial region-specific effects on bone-tendon healing in vitro, such as enhanced expression of parameters for ossification and fibroblast transdifferentiation, both key processes during successful graft integration.
PURPOSE: Bone-tendon healing following anterior cruciate ligament reconstruction is reportedly enhanced by bone morphogenetic protein (BMP)-7. To improve our understanding of the underlying biologic processes, we examined the effects of BMP-7 on region-specific gene expression in vitro. METHODS: A murine in vitro co-culture model simulating the osteoblast, interface and fibroblast regions was established. The dose- and time-dependent region-specific effects of BMP-7 exposure on gene expression of Alpl, Bglap, Col1a1, Runx2 and Spp1 were analysed by quantitative PCR. RESULTS: At the osteoblast region, BMP-7 significantly increased Alp, Bglap, Col1a1, and Runx2 expression, while Spp1 expression was suppressed. At the interface region, BMP-7 exposure resulted in a trend towards increased expression rates of Alpl and Col1a1, whereas Bglap (P < 0.001) and Runx2 (P < 0.01) were significantly upregulated without any detectable effect on Spp1 expression. At the fibroblast region, BMP-7 increased Alpl (P < 0.001), Bglap (P < 0.001) and Runx2 (P < 0.001) expression, but no significant effects were seen on Col1a1 or Spp1. Exposure to BMP-7 (100 ng/ml) had its most pronounced biologic impact on day ten. CONCLUSION:BMP-7 stimulation showed beneficial region-specific effects on bone-tendon healing in vitro, such as enhanced expression of parameters for ossification and fibroblast transdifferentiation, both key processes during successful graft integration.
Authors: I-Ning E Wang; Jing Shan; Rene Choi; Seongcheol Oh; Christopher K Kepler; Faye H Chen; Helen H Lu Journal: J Orthop Res Date: 2007-12 Impact factor: 3.494
Authors: Vladimir Martinek; Christian Latterman; Arvydas Usas; Steven Abramowitch; Savio L-Y Woo; Freddie H Fu; Johnny Huard Journal: J Bone Joint Surg Am Date: 2002-07 Impact factor: 5.284
Authors: Nathan A Mall; Peter N Chalmers; Mario Moric; Miho J Tanaka; Brian J Cole; Bernard R Bach; George A Paletta Journal: Am J Sports Med Date: 2014-08-01 Impact factor: 6.202
Authors: Franka Klatte-Schulz; Stephan Pauly; Markus Scheibel; Stefan Greiner; Christian Gerhardt; Jelka Hartwig; Gerhard Schmidmaier; Britt Wildemann Journal: PLoS One Date: 2013-06-25 Impact factor: 3.240
Authors: Vladimir J Cvetković; Jelena G Najdanović; Marija Đ Vukelić-Nikolić; Sanja Stojanović; Stevo J Najman Journal: Int Orthop Date: 2015-08-01 Impact factor: 3.075