Wen Guo1, Jinxiang Fu2, Xiaoli Chen2, Beibei Gao2, Zhenzhen Fu2, Hongqi Fan2, Qin Cui3, Xiaohui Zhu2, Yang Zhao4, Tao Yang2, Daping Fan5, Hongwen Zhou6. 1. Department of Endocrinology, The First Affiliated Hospital of Nanjing Medical University, Nanjing 210029, China; Department of Endocrinology, Nanjing Municipal Hospital for Governmental Organizations, Nanjing 210018, China. 2. Department of Endocrinology, The First Affiliated Hospital of Nanjing Medical University, Nanjing 210029, China. 3. Department of Geriatrics, Department of Cadres, Tong Ling People's Hospital, Tongling 244009, China. 4. School of Public Health Nanjing Medical University, Nanjing 210029, China. 5. Department of Cell Biology and Anatomy, School of Medicine, University of South Carolina, 6439 Garners Ferry Road, Columbia, SC 29209, United States. 6. Department of Endocrinology, The First Affiliated Hospital of Nanjing Medical University, Nanjing 210029, China. Electronic address: drhongwenzhou@njmu.edu.cn.
Abstract
OBJECTIVE: We previously reported that serum PCSK9 levels are higher in postmenopausal women than in premenopausal women in a Han Chinese population. Whether this difference is related to estrogen has not been well-characterized. This study aims to examine if the alteration in estrogen level is responsible for the changes of serum PCSK9 concentration. MATERIALS/ METHODS: A sandwich ELISA assay was used to measure serum PCSK9 levels in 727 healthy women aged from 26 to 85 years old. Anthropometric and biochemical examination of parameters such as estrogen and serum lipids was also performed for these individuals. Next, we measured serum PCSK9 and estrogen levels of 30 healthy fertile women (24-26 years old) in their menstrual cycles and analyzed the correlation between serum PCSK9 level and estrogen concentration. Moreover, cell culture studies were carried out to examine if estrogen at physiological and non-physiological concentrations regulates hepatocyte PCSK9 expression. RESULTS: Serum PCSK9 concentrations were significantly increased with aging. Aged group had higher serum PCSK9 levels than the middle aged group and the young group (60.29±28.47 vs 71.38±34.22 vs 83.81±33.50 ng/ml). Serum PCSK9 levels were positively correlated with age, BMI, serum total cholesterol and LDL-cholesterol (P<0.01), but not correlated with estrogen levels. There was no significantly difference of PCSK9 levels between the lower and the upper estradiol (E2) tertiles in the 727 women. There was either no significant difference in PCSK9 levels during the menstrual, ovulatory, luteal phases in the 30 healthy fertile women. Cell culture studies showed that 17β-estradiol at physiological concentrations did not significantly alter PCSK9 expression in human hepatocytes. CONCLUSION: The serum PCSK9 levels were higher in postmenopausal women than those in pre-menopausal women. However, the difference in serum PCSK9 levels between postmenopausal and premenopausal woman appeared to be independent of estrogen status, and estrogen at physiological concentrations does not affect human hepatocyte PCSK9 expression.
OBJECTIVE: We previously reported that serum PCSK9 levels are higher in postmenopausal women than in premenopausal women in a Han Chinese population. Whether this difference is related to estrogen has not been well-characterized. This study aims to examine if the alteration in estrogen level is responsible for the changes of serum PCSK9 concentration. MATERIALS/ METHODS: A sandwich ELISA assay was used to measure serum PCSK9 levels in 727 healthy women aged from 26 to 85 years old. Anthropometric and biochemical examination of parameters such as estrogen and serum lipids was also performed for these individuals. Next, we measured serum PCSK9 and estrogen levels of 30 healthy fertile women (24-26 years old) in their menstrual cycles and analyzed the correlation between serum PCSK9 level and estrogen concentration. Moreover, cell culture studies were carried out to examine if estrogen at physiological and non-physiological concentrations regulates hepatocyte PCSK9 expression. RESULTS: Serum PCSK9 concentrations were significantly increased with aging. Aged group had higher serum PCSK9 levels than the middle aged group and the young group (60.29±28.47 vs 71.38±34.22 vs 83.81±33.50 ng/ml). Serum PCSK9 levels were positively correlated with age, BMI, serum total cholesterol and LDL-cholesterol (P<0.01), but not correlated with estrogen levels. There was no significantly difference of PCSK9 levels between the lower and the upper estradiol (E2) tertiles in the 727 women. There was either no significant difference in PCSK9 levels during the menstrual, ovulatory, luteal phases in the 30 healthy fertile women. Cell culture studies showed that 17β-estradiol at physiological concentrations did not significantly alter PCSK9 expression in human hepatocytes. CONCLUSION: The serum PCSK9 levels were higher in postmenopausal women than those in pre-menopausal women. However, the difference in serum PCSK9 levels between postmenopausal and premenopausal woman appeared to be independent of estrogen status, and estrogen at physiological concentrations does not affect human hepatocyte PCSK9 expression.
Authors: Amy E Levenson; Amy S Shah; Philip R Khoury; Thomas R Kimball; Elaine M Urbina; Sarah D de Ferranti; David M Maahs; Lawrence M Dolan; R Paul Wadwa; Sudha B Biddinger Journal: Pediatr Diabetes Date: 2017-01-17 Impact factor: 4.866
Authors: Jie Shi; Xiaoyong Li; Weiwei Zhang; Yixin Niu; Ning Lin; Hongmei Zhang; Guang Ning; Jiangao Fan; Li Qin; Qing Su; Zhen Yang Journal: Front Cardiovasc Med Date: 2021-05-10