Literature DB >> 25665438

Methods for monitoring lysosomal morphology.

Bethan S Kilpatrick1, Emily R Eden2, Leanne N Hockey1, Clare E Futter2, Sandip Patel1.   

Abstract

Lysosomes are abundant organelles best known for their crucial role in macromolecule turnover. Lysosome dysfunction features in several diseases exemplified by the lysosomal storage disorders and is often associated with marked changes in lysosome structure. Lysosomal morphology may therefore serve as a sensitive readout of endocytic well-being. Here we describe methods for monitoring lysosome morphology in fixed and live cells using fluorescent probes and electron microscopy.
Copyright © 2015 Elsevier Inc. All rights reserved.

Entities:  

Keywords:  Electron microscopy; Endocytosis; Fluorescence microscopy; Immunofluorescence; LAMP1; Lysosome; Membrane traffic; NAADP; Two-pore channels

Mesh：

Substances：

Year:  2015        PMID： 25665438     DOI： 10.1016/bs.mcb.2014.10.018

Source DB:  PubMed          Journal:  Methods Cell Biol        ISSN： 0091-679X            Impact factor:   1.441


  10 in total

1.  Loss of Sirtuin 1 Alters the Secretome of Breast Cancer Cells by Impairing Lysosomal Integrity.

Authors:  Arash Latifkar; Lu Ling; Amrit Hingorani; Eric Johansen; Amdiel Clement; Xiaoyu Zhang; John Hartman; Claudia Fischbach; Hening Lin; Richard A Cerione; Marc A Antonyak
Journal:  Dev Cell       Date:  2019-04-11       Impact factor: 12.270

2.  Lysosome triggered near-infrared fluorescence imaging of cellular trafficking processes in real time.

Authors:  Marco Grossi; Marina Morgunova; Shane Cheung; Dimitri Scholz; Emer Conroy; Marta Terrile; Angela Panarella; Jeremy C Simpson; William M Gallagher; Donal F O'Shea
Journal:  Nat Commun       Date:  2016-03-01       Impact factor: 14.919

3.  Endoplasmic reticulum and lysosomal Ca²⁺ stores are remodelled in GBA1-linked Parkinson disease patient fibroblasts.

Authors:  Bethan S Kilpatrick; Joana Magalhaes; Michelle S Beavan; Alisdair McNeill; Matthew E Gegg; Michael W J Cleeter; Duncan Bloor-Young; Grant C Churchill; Michael R Duchen; Anthony H Schapira; Sandip Patel
Journal:  Cell Calcium       Date:  2015-11-26       Impact factor: 6.817

Review 4.  Autophagy in Neurotrauma: Good, Bad, or Dysregulated.

Authors:  Junfang Wu; Marta M Lipinski
Journal:  Cells       Date:  2019-07-10       Impact factor: 6.600

5.  PLA2G4A/cPLA2-mediated lysosomal membrane damage leads to inhibition of autophagy and neurodegeneration after brain trauma.

Authors:  Chinmoy Sarkar; Jace W Jones; Nivedita Hegdekar; Julia A Thayer; Alok Kumar; Alan I Faden; Maureen A Kane; Marta M Lipinski
Journal:  Autophagy       Date:  2019-06-25       Impact factor: 16.016

Review 6.  Current methods to analyze lysosome morphology, positioning, motility and function.

Authors:  Duarte C Barral; Leopoldo Staiano; Cláudia Guimas Almeida; Dan F Cutler; Emily R Eden; Clare E Futter; Antony Galione; André R A Marques; Diego Luis Medina; Gennaro Napolitano; Carmine Settembre; Otília V Vieira; Johannes M F G Aerts; Peace Atakpa-Adaji; Gemma Bruno; Antonella Capuozzo; Elvira De Leonibus; Chiara Di Malta; Cristina Escrevente; Alessandra Esposito; Paolo Grumati; Michael J Hall; Rita O Teodoro; Susana S Lopes; J Paul Luzio; Jlenia Monfregola; Sandro Montefusco; Frances M Platt; Roman Polishchuck; Maria De Risi; Irene Sambri; Chiara Soldati; Miguel C Seabra
Journal:  Traffic       Date:  2022-04-24       Impact factor: 6.144

7.  α-Synuclein Overexpression Induces Lysosomal Dysfunction and Autophagy Impairment in Human Neuroblastoma SH-SY5Y.

Authors:  Ana Carolina Nascimento; Adolfo G Erustes; Patrícia Reckziegel; Claudia Bincoletto; Rodrigo P Ureshino; Gustavo J S Pereira; Soraya S Smaili
Journal:  Neurochem Res       Date:  2020-09-11       Impact factor: 3.996

8.  Lysosomal retargeting of Myoferlin mitigates membrane stress to enable pancreatic cancer growth.

Authors:  Suprit Gupta; Julian Yano; Vincent Mercier; Htet Htwe Htwe; Hijai R Shin; Gilles Rademaker; Zeynep Cakir; Thomas Ituarte; Kwun W Wen; Grace E Kim; Roberto Zoncu; Aurélien Roux; David W Dawson; Rushika M Perera
Journal:  Nat Cell Biol       Date:  2021-03-08       Impact factor: 28.213

9.  Ca2+/H+ exchange by acidic organelles regulates cell migration in vivo.

Authors:  Manuela Melchionda; Jon K Pittman; Roberto Mayor; Sandip Patel
Journal:  J Cell Biol       Date:  2016-03-21       Impact factor: 10.539

10.  A Carbon Nanotube Optical Reporter Maps Endolysosomal Lipid Flux.

Authors:  Prakrit V Jena; Daniel Roxbury; Thomas V Galassi; Leila Akkari; Christopher P Horoszko; David B Iaea; Januka Budhathoki-Uprety; Nina Pipalia; Abigail S Haka; Jackson D Harvey; Jeetain Mittal; Frederick R Maxfield; Johanna A Joyce; Daniel A Heller
Journal:  ACS Nano       Date:  2017-09-12       Impact factor: 15.881
  10 in total

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