| Literature DB >> 25665008 |
Daniel C Jeffares1, Charalampos Rallis1, Adrien Rieux2, Doug Speed2, Martin Převorovský3, Tobias Mourier4, Francesc X Marsellach1, Zamin Iqbal5, Winston Lau1, Tammy M K Cheng6, Rodrigo Pracana1, Michael Mülleder7, Jonathan L D Lawson8, Anatole Chessel7, Sendu Bala9, Garrett Hellenthal2, Brendan O'Fallon10, Thomas Keane9, Jared T Simpson9, Leanne Bischof11, Bartlomiej Tomiczek1, Danny A Bitton1, Theodora Sideri1, Sandra Codlin1, Josephine E E U Hellberg1, Laurent van Trigt1, Linda Jeffery6, Juan-Juan Li6, Sophie Atkinson1, Malte Thodberg4, Melanie Febrer12, Kirsten McLay12, Nizar Drou12, William Brown13, Jacqueline Hayles6, Rafael E Carazo Salas8, Markus Ralser14, Nikolas Maniatis1, David J Balding2, Francois Balloux2, Richard Durbin9, Jürg Bähler2.
Abstract
Natural variation within species reveals aspects of genome evolution and function. The fission yeast Schizosaccharomyces pombe is an important model for eukaryotic biology, but researchers typically use one standard laboratory strain. To extend the usefulness of this model, we surveyed the genomic and phenotypic variation in 161 natural isolates. We sequenced the genomes of all strains, finding moderate genetic diversity (π = 3 × 10(-3) substitutions/site) and weak global population structure. We estimate that dispersal of S. pombe began during human antiquity (∼340 BCE), and ancestors of these strains reached the Americas at ∼1623 CE. We quantified 74 traits, finding substantial heritable phenotypic diversity. We conducted 223 genome-wide association studies, with 89 traits showing at least one association. The most significant variant for each trait explained 22% of the phenotypic variance on average, with indels having larger effects than SNPs. This analysis represents a rich resource to examine genotype-phenotype relationships in a tractable model.Entities:
Mesh：
Year: 2015 PMID： 25665008 PMCID： PMC4645456 DOI： 10.1038/ng.3215
Source DB: PubMed Journal: Nat Genet ISSN： 1061-4036 Impact factor: 38.330