Yue Xu1, Mengdie Yan1, Yihong Gong2, Lei Chen3, Feng Zhao4, Zhaoqiang Zhang5. 1. Department of Orthodontics, Guanghua School of Stomatology, Hospital of Stomatology, Sun Yat-sen University, Guangdong Provincial Key Laboratory of Stomatology Guangzhou, Guangdong, China. 2. School of Engineering, Sun Yat-sen University Guangzhou, Guangdong, China. 3. Department of Burns Surgery, First Affiliated Hospital of Sun Yat-sen University Guangzhou, Guangdong, China. 4. Department of Biomedical Engineering, Michigan Technological University 1400 Townsend Drive, Houghton, Michigan, USA. 5. Department of Stomatology, Sixth Affiliated Hospital of Sun Yat-sen University Guangzhou, Guangdong, China.
Abstract
OBJECTIVE: Evaluate the behavior and function of human umbilical vein endothelial cells (HUVECs) on decellularized extracellular matrix (ECM) deposited by bone marrow mesenchymal stem cells (BMSCs). METHODS: Prepared through chemical approach, decellularized ECM was characterized by use of immunofluorescence staining. The morphology, attachment, proliferation and migration of HUVECs cultured on six-well tissue culture plastic (TCP) and decellularized ECM were investigated. RESULTS: Decellularized ECM was successfully prepared without three-dimensional architecture disruption. This biological scaffold is similar to nature vascular ECM, preserved various matrix proteins such as type I collagen, type III collagen and fibronection. HUVECs on decellularized ECM showed well attachment and regular arrangement. Decellularized ECM could also significantly enhance the migration and proliferation potential of HUVECs in contrast to TCP. CONCLUSION: Deposited by BMSCs, ECM can affect the behavior of endothelial cell and could be used as a promising material in tissue engineering.
OBJECTIVE: Evaluate the behavior and function of human umbilical vein endothelial cells (HUVECs) on decellularized extracellular matrix (ECM) deposited by bone marrow mesenchymal stem cells (BMSCs). METHODS: Prepared through chemical approach, decellularized ECM was characterized by use of immunofluorescence staining. The morphology, attachment, proliferation and migration of HUVECs cultured on six-well tissue culture plastic (TCP) and decellularized ECM were investigated. RESULTS: Decellularized ECM was successfully prepared without three-dimensional architecture disruption. This biological scaffold is similar to nature vascular ECM, preserved various matrix proteins such as type I collagen, type III collagen and fibronection. HUVECs on decellularized ECM showed well attachment and regular arrangement. Decellularized ECM could also significantly enhance the migration and proliferation potential of HUVECs in contrast to TCP. CONCLUSION: Deposited by BMSCs, ECM can affect the behavior of endothelial cell and could be used as a promising material in tissue engineering.
Authors: Lisa E Freed; Farshid Guilak; X Edward Guo; Martha L Gray; Robert Tranquillo; Jeffrey W Holmes; Milica Radisic; Michael V Sefton; David Kaplan; Gordana Vunjak-Novakovic Journal: Tissue Eng Date: 2006-12
Authors: Meghan L Milburn; Luther H Holton; Thomas L Chung; Edward N Li; Grant V Bochicchio; Nelson H Goldberg; Ronald P Silverman Journal: Surg Infect (Larchmt) Date: 2008-08 Impact factor: 2.150