Juliano Milanezi de Almeida1, Alvaro Francisco Bosco2, Paula Lazilha Faleiros3, Victor Fabrizio Cabrera Pazmiño4, Ana Cristina Murakawa5, Samara Bonfante6, Maria José Hitomi Nagata7, Valdir Gouveia Garcia8. 1. Univ. Estadual Paulista - UNESP, Division of Periodontics, Department of Surgery and Integrated Clinic, Dental School of Araçatuba, Rua José Bonifácio, n° 1193, CEP: 16015-050, Araçatuba, São Paulo, Brazil. Electronic address: jumilanezi@hotmail.com. 2. Univ. Estadual Paulista - UNESP, Division of Periodontics, Department of Surgery and Integrated Clinic, Dental School of Araçatuba, Rua José Bonifácio, n° 1193, CEP: 16015-050, Araçatuba, São Paulo, Brazil. Electronic address: afbosco@hotmail.com. 3. Univ. Estadual Paulista - UNESP, Division of Periodontics, Department of Surgery and Integrated Clinic, Dental School of Araçatuba, Rua José Bonifácio, n° 1193, CEP: 16015-050, Araçatuba, São Paulo, Brazil. Electronic address: paulal.faleiros@hotmail.com. 4. Univ. Estadual Paulista - UNESP, Division of Periodontics, Department of Surgery and Integrated Clinic, Dental School of Araçatuba, Rua José Bonifácio, n° 1193, CEP: 16015-050, Araçatuba, São Paulo, Brazil. Electronic address: drfabriziocabrera@hotmail.com. 5. Univ. Estadual Paulista - UNESP, Division of Periodontics, Department of Surgery and Integrated Clinic, Dental School of Araçatuba, Rua José Bonifácio, n° 1193, CEP: 16015-050, Araçatuba, São Paulo, Brazil. Electronic address: a_murakawa@hotmail.com. 6. Univ. Estadual Paulista - UNESP, Division of Periodontics, Department of Surgery and Integrated Clinic, Dental School of Araçatuba, Rua José Bonifácio, n° 1193, CEP: 16015-050, Araçatuba, São Paulo, Brazil. Electronic address: samarabonfante@uol.com. 7. Univ. Estadual Paulista - UNESP, Division of Periodontics, Department of Surgery and Integrated Clinic, Dental School of Araçatuba, Rua José Bonifácio, n° 1193, CEP: 16015-050, Araçatuba, São Paulo, Brazil. Electronic address: mjnagata@uol.com.br. 8. Univ. Estadual Paulista - UNESP, Division of Periodontics, Department of Surgery and Integrated Clinic, Dental School of Araçatuba, Rua José Bonifácio, n° 1193, CEP: 16015-050, Araçatuba, São Paulo, Brazil; University Center of Educational Foundation of Barretos - UNIFEB, Department of Periodontics, Dental School of Barretos, Avenida Professor Roberto Frade Monte, n° 389, CEP: 14783-226, Barretos, São Paulo, Brazil. Electronic address: vg.garcia@uol.com.br.
Abstract
OBJECTIVE: To histomorphometrically analyze the effect of ovariectomy-induced oestrogen deficiency and 17β-estradiol therapy on bone healing of surgically created critical-size defects (CSDs) treated with bovine bone graft (BBG). METHODS: Forty-eight female rats were randomly assigned to the following 3 experimental groups (n=16): sham-operated animals (SHAM), ovariectomized animals (OVX) and ovariectomized animals treated with oestrogen (OVX+E2). OVX+E2 animals received daily subcutaneous injections of 17β-estradiol (20μg/kg) from 8 days after ovariectomy until euthanasia. Thirty days after the surgery, an 8mm CSD was surgically created in each calvaria of all animals and filled with BBG. Animals were euthanized at either 30 or 60 days postoperative. A histological analysis, percentage of Newly Formed Bone Area (NFBA), osteoblast and osteoclast number was histomorphometrically performed (p≤0.05). RESULTS: At 30 days, SHAM group (8.82%±2.93) had significantly greater NFBA than OVX (4.66%±1.35) and OVX+E2 groups (5.85%±4.08) (p≤0.05). At 60 days, SHAM group (11.51%±3.08) and OVX+E2 group (9.84%±1.87) had significantly greater NFBA than OVX animals (5.12%±0.68) (p≤0.05). Fewer osteoblasts were observed in the OVX group at 30 (763.40±121.60) and 60 (696.60±80.92) days than in the SHAM group at 30 days (1356.00±95.38). Fewer osteoclasts were observed in the OVX+E2 group (3.25±2.16) than in the SHAM (9.75±1.82) and OVX (12.75±1.47) groups at 30 days (p≤0.05). CONCLUSIONS: Oestrogen deficiency compromises bone healing in calvarial CSDs treated with BBG in ovariectomized rats. After 60 days post-surgery, 17β-estradiol therapy improved bone healing in calvarial CSDs treated with BBG in ovariectomized rats.
OBJECTIVE: To histomorphometrically analyze the effect of ovariectomy-induced oestrogen deficiency and 17β-estradiol therapy on bone healing of surgically created critical-size defects (CSDs) treated with bovine bone graft (BBG). METHODS: Forty-eight female rats were randomly assigned to the following 3 experimental groups (n=16): sham-operated animals (SHAM), ovariectomized animals (OVX) and ovariectomized animals treated with oestrogen (OVX+E2). OVX+E2 animals received daily subcutaneous injections of 17β-estradiol (20μg/kg) from 8 days after ovariectomy until euthanasia. Thirty days after the surgery, an 8mm CSD was surgically created in each calvaria of all animals and filled with BBG. Animals were euthanized at either 30 or 60 days postoperative. A histological analysis, percentage of Newly Formed Bone Area (NFBA), osteoblast and osteoclast number was histomorphometrically performed (p≤0.05). RESULTS: At 30 days, SHAM group (8.82%±2.93) had significantly greater NFBA than OVX (4.66%±1.35) and OVX+E2 groups (5.85%±4.08) (p≤0.05). At 60 days, SHAM group (11.51%±3.08) and OVX+E2 group (9.84%±1.87) had significantly greater NFBA than OVX animals (5.12%±0.68) (p≤0.05). Fewer osteoblasts were observed in the OVX group at 30 (763.40±121.60) and 60 (696.60±80.92) days than in the SHAM group at 30 days (1356.00±95.38). Fewer osteoclasts were observed in the OVX+E2 group (3.25±2.16) than in the SHAM (9.75±1.82) and OVX (12.75±1.47) groups at 30 days (p≤0.05). CONCLUSIONS: Oestrogen deficiency compromises bone healing in calvarial CSDs treated with BBG in ovariectomized rats. After 60 days post-surgery, 17β-estradiol therapy improved bone healing in calvarial CSDs treated with BBG in ovariectomized rats.
Authors: Elisabet Segredo-Morales; Ricardo Reyes; María Rosa Arnau; Araceli Delgado; Carmen Évora Journal: Drug Deliv Transl Res Date: 2018-10 Impact factor: 4.617
Authors: Jan D Raguse; Ioannis Anagnostopoulos; Christian Doll; Max Heiland; Korinna Jöhrens Journal: Biomed Res Int Date: 2017-12-19 Impact factor: 3.411