Literature DB >> 25660532

Sandwich enzyme-linked immunosorbent assay for the quantification of human serum albumin fragment 408-423 in bodily fluids.

Katharina B Mohr1, Onofrio Zirafi2, Mark Hennies3, Sebastian Wiese4, Frank Kirchhoff5, Jan Münch6.   

Abstract

Urinary levels of human serum albumin (hSA) fragment 408-423 have been proposed to represent an early marker for graft-versus-host disease (GvHD) and chronic kidney diseases. Here, we developed an enzyme-linked immunosorbent assay (ELISA) for the quantification of hSA(408-423). The sandwich ELISA has a detection limit of 0.5ng/ml and is highly specific for hSA(408-423) because it does not cross-react with other albumin fragments or the full-length precursor. This ELISA allows rapid and convenient quantification of hSA(408-423) in bodily fluids, further clarifying the prognostic and diagnostic value of this peptide in GvHD, kidney disease, and other disorders.
Copyright © 2015 Elsevier Inc. All rights reserved.

Entities:  

Keywords:  Biomarker; ELISA; Graft-versus-host disease; Human serum albumin; Kidney disease; Peptide

Mesh：

Substances：

Year:  2015        PMID： 25660532     DOI： 10.1016/j.ab.2015.01.023

Source DB:  PubMed          Journal:  Anal Biochem        ISSN： 0003-2697            Impact factor:   3.365


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