Literature DB >> 25659908

The Pex1/Pex6 complex is a heterohexameric AAA+ motor with alternating and highly coordinated subunits.

Brooke M Gardner1, Saikat Chowdhury2, Gabriel C Lander2, Andreas Martin3.   

Abstract

Pex1 and Pex6 are Type-2 AAA+ ATPases required for the de novo biogenesis of peroxisomes. Mutations in Pex1 and Pex6 account for the majority of the most severe forms of peroxisome biogenesis disorders in humans. Here, we show that the ATP-dependent complex of Pex1 and Pex6 from Saccharomyces cerevisiae is a heterohexamer with alternating subunits. Within the Pex1/Pex6 complex, only the D2 ATPase ring hydrolyzes ATP, while nucleotide binding in the D1 ring promotes complex assembly. ATP hydrolysis by Pex1 is highly coordinated with that of Pex6. Furthermore, Pex15, the membrane anchor required for Pex1/Pex6 recruitment to peroxisomes, inhibits the ATP-hydrolysis activity of Pex1/Pex6.
Copyright © 2015 Elsevier Ltd. All rights reserved.

Entities:  

Keywords:  AAA+ ATPase; Pex1; Pex15; Pex6; peroxisome

Mesh：

Substances：

Year:  2015        PMID： 25659908      PMCID： PMC4355278          DOI： 10.1016/j.jmb.2015.01.019

Source DB:  PubMed          Journal:  J Mol Biol        ISSN： 0022-2836            Impact factor:   5.469


  77 in total

1.  EMAN: semiautomated software for high-resolution single-particle reconstructions.

Authors:  S J Ludtke; P R Baldwin; W Chiu
Journal:  J Struct Biol       Date:  1999-12-01       Impact factor: 2.867

2.  Structure of the AAA ATPase p97.

Authors:  X Zhang; A Shaw; P A Bates; R H Newman; B Gowen; E Orlova; M A Gorman; H Kondo; P Dokurno; J Lally; G Leonard; H Meyer; M van Heel; P S Freemont
Journal:  Mol Cell       Date:  2000-12       Impact factor: 17.970

3.  ATP-dependent assembly of the heteromeric Pex1p-Pex6p-complex of the peroxisomal matrix protein import machinery.

Authors:  Delia Saffian; Immanuel Grimm; Wolfgang Girzalsky; Ralf Erdmann
Journal:  J Struct Biol       Date:  2012-06-16       Impact factor: 2.867

Review 4.  The peroxisomal receptor dislocation pathway: to the exportomer and beyond.

Authors:  Harald W Platta; Stefanie Hagen; Christina Reidick; Ralf Erdmann
Journal:  Biochimie       Date:  2013-12-15       Impact factor: 4.079

Review 5.  Peroxisome formation and maintenance are dependent on the endoplasmic reticulum.

Authors:  Henk F Tabak; Ineke Braakman; Adabella van der Zand
Journal:  Annu Rev Biochem       Date:  2013-02-14       Impact factor: 23.643

6.  Peroxisomal membrane fusion requires two AAA family ATPases, Pex1p and Pex6p.

Authors:  V I Titorenko; R A Rachubinski
Journal:  J Cell Biol       Date:  2000-08-21       Impact factor: 10.539

7.  Fusion of small peroxisomal vesicles in vitro reconstructs an early step in the in vivo multistep peroxisome assembly pathway of Yarrowia lipolytica.

Authors:  V I Titorenko; H Chan; R A Rachubinski
Journal:  J Cell Biol       Date:  2000-01-10       Impact factor: 10.539

8.  Reconstitution of the 26S proteasome reveals functional asymmetries in its AAA+ unfoldase.

Authors:  Robyn Beckwith; Eric Estrin; Evan J Worden; Andreas Martin
Journal:  Nat Struct Mol Biol       Date:  2013-09-08       Impact factor: 15.369

9.  RELION: implementation of a Bayesian approach to cryo-EM structure determination.

Authors:  Sjors H W Scheres
Journal:  J Struct Biol       Date:  2012-09-19       Impact factor: 2.867

10.  Peb1p (Pas7p) is an intraperoxisomal receptor for the NH2-terminal, type 2, peroxisomal targeting sequence of thiolase: Peb1p itself is targeted to peroxisomes by an NH2-terminal peptide.

Authors:  J W Zhang; P B Lazarow
Journal:  J Cell Biol       Date:  1996-02       Impact factor: 10.539
View more
  28 in total

1.  Peroxisomal monoubiquitinated PEX5 interacts with the AAA ATPases PEX1 and PEX6 and is unfolded during its dislocation into the cytosol.

Authors:  Ana G Pedrosa; Tânia Francisco; Diana Bicho; Ana F Dias; Aurora Barros-Barbosa; Vera Hagmann; Gabriele Dodt; Tony A Rodrigues; Jorge E Azevedo
Journal:  J Biol Chem       Date:  2018-06-08       Impact factor: 5.157

2.  Unique double-ring structure of the peroxisomal Pex1/Pex6 ATPase complex revealed by cryo-electron microscopy.

Authors:  Neil B Blok; Dongyan Tan; Ray Yu-Ruei Wang; Pawel A Penczek; David Baker; Frank DiMaio; Tom A Rapoport; Thomas Walz
Journal:  Proc Natl Acad Sci U S A       Date:  2015-07-13       Impact factor: 11.205

3.  Receptor recognition by the peroxisomal AAA complex depends on the presence of the ubiquitin moiety and is mediated by Pex1p.

Authors:  Daniel Schwerter; Immanuel Grimm; Wolfgang Girzalsky; Ralf Erdmann
Journal:  J Biol Chem       Date:  2018-08-10       Impact factor: 5.157

Review 4.  Assessing heterogeneity in oligomeric AAA+ machines.

Authors:  Tatyana A Sysoeva
Journal:  Cell Mol Life Sci       Date:  2016-09-26       Impact factor: 9.261

5.  Characterization of the heterooligomeric red-type rubisco activase from red algae.

Authors:  Nitin Loganathan; Yi-Chin Candace Tsai; Oliver Mueller-Cajar
Journal:  Proc Natl Acad Sci U S A       Date:  2016-11-21       Impact factor: 11.205

Review 6.  Peroxisome Function, Biogenesis, and Dynamics in Plants.

Authors:  Yun-Ting Kao; Kim L Gonzalez; Bonnie Bartel
Journal:  Plant Physiol       Date:  2017-10-11       Impact factor: 8.340

7.  Disparate peroxisome-related defects in Arabidopsis pex6 and pex26 mutants link peroxisomal retrotranslocation and oil body utilization.

Authors:  Kim L Gonzalez; Wendell A Fleming; Yun-Ting Kao; Zachary J Wright; Savina V Venkova; Meredith J Ventura; Bonnie Bartel
Journal:  Plant J       Date:  2017-08-22       Impact factor: 6.417

8.  The PEX1 ATPase Stabilizes PEX6 and Plays Essential Roles in Peroxisome Biology.

Authors:  Mauro A Rinaldi; Wendell A Fleming; Kim L Gonzalez; Jaeseok Park; Meredith J Ventura; Ashish B Patel; Bonnie Bartel
Journal:  Plant Physiol       Date:  2017-06-09       Impact factor: 8.340

9.  A pex1 missense mutation improves peroxisome function in a subset of Arabidopsis pex6 mutants without restoring PEX5 recycling.

Authors:  Kim L Gonzalez; Sarah E Ratzel; Kendall H Burks; Charles H Danan; Jeanne M Wages; Bethany K Zolman; Bonnie Bartel
Journal:  Proc Natl Acad Sci U S A       Date:  2018-03-19       Impact factor: 11.205

10.  Peroxisomal targeting of a protein phosphatase type 2C via mitochondrial transit.

Authors:  Thorsten Stehlik; Marco Kremp; Jörg Kahnt; Michael Bölker; Johannes Freitag
Journal:  Nat Commun       Date:  2020-05-12       Impact factor: 14.919
View more

北京卡尤迪生物科技股份有限公司 © 2022-2023.