Optical analysis of biological hydrogen sulphide: an overview of recent advancements.

Determining the levels of endogenous hydrogen sulphide in real time has become increasingly crucial because of its important biological roles in various physiological and pathological processes. Optical methods allowing sensitive, multiplex and dynamic analysis in a non-invasive manner have attracted much attention in biological and biomedical analysis. This review provides an overview of recent advancements in optical analysis of biological hydrogen sulphide, with a focus on fluorescence and non-fluorescence optical strategies for sensing and imaging subcellular hydrogen sulphide in living biosystems.