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Literature DB >> 2565023

Polymerase chain reaction: replication errors and reliability of gene diagnosis.

M Krawczak¹, J Reiss, J Schmidtke, U Rösler.

Abstract

The impact of replication errors on the reliability of polymerase chain reaction (PCR) data is studied theoretically. Practical applications of our results to RFLP analysis and oligonucleotide probing confirm that for practical purposes replication errors can be neglected if a large number of starting templates (e.g. 100,000) is being used. For single locus analysis in single cells, however, the probability of false diagnosis due to such errors is of the order of 1 percent.

Entities: Chemical

Mesh：

Substances：

Year: 1989 PMID： 2565023 PMCID： PMC317589 DOI： 10.1093/nar/17.6.2197

Source DB: PubMed Journal: Nucleic Acids Res ISSN： 0305-1048 Impact factor: 16.971

7 in total

1. Fidelity of DNA synthesis by the Thermus aquaticus DNA polymerase.

Authors: K R Tindall; T A Kunkel
Journal: Biochemistry Date: 1988-08-09 Impact factor: 3.162

2. Enzymatic amplification of beta-globin genomic sequences and restriction site analysis for diagnosis of sickle cell anemia.

Authors: R K Saiki; S Scharf; F Faloona; K B Mullis; G T Horn; H A Erlich; N Arnheim
Journal: Science Date: 1985-12-20 Impact factor: 47.728

3. Primer-directed enzymatic amplification of DNA with a thermostable DNA polymerase.

Authors: R K Saiki; D H Gelfand; S Stoffel; S J Scharf; R Higuchi; G T Horn; K B Mullis; H A Erlich
Journal: Science Date: 1988-01-29 Impact factor: 47.728

4. Deoxyribonucleic acid polymerase from the extreme thermophile Thermus aquaticus.

Authors: A Chien; D B Edgar; J M Trela
Journal: J Bacteriol Date: 1976-09 Impact factor: 3.490

5. Direct cloning and sequence analysis of enzymatically amplified genomic sequences.

Authors: S J Scharf; G T Horn; H A Erlich
Journal: Science Date: 1986-09-05 Impact factor: 47.728

6. Errors in the polymerase chain reaction.

Authors: A M Dunning; P Talmud; S E Humphries
Journal: Nucleic Acids Res Date: 1988-11-11 Impact factor: 16.971

7. Diagnosis of alpha 1-antitrypsin deficiency by enzymatic amplification of human genomic DNA and direct sequencing of polymerase chain reaction products.

Authors: C R Newton; N Kalsheker; A Graham; S Powell; A Gammack; J Riley; A F Markham
Journal: Nucleic Acids Res Date: 1988-09-12 Impact factor: 16.971

7 in total

24 in total

10. An infectious cDNA copy of the genome of a non-cardiovirulent coxsackievirus B3 strain: its complete sequence analysis and comparison to the genomes of cardiovirulent coxsackieviruses.

Authors: N M Chapman; Z Tu; S Tracy; C J Gauntt
Journal: Arch Virol Date: 1994 Impact factor: 2.574

Polymerase chain reaction: replication errors and reliability of gene diagnosis.

1. Fidelity of DNA synthesis by the Thermus aquaticus DNA polymerase.

2. Enzymatic amplification of beta-globin genomic sequences and restriction site analysis for diagnosis of sickle cell anemia.

3. Primer-directed enzymatic amplification of DNA with a thermostable DNA polymerase.

4. Deoxyribonucleic acid polymerase from the extreme thermophile Thermus aquaticus.

5. Direct cloning and sequence analysis of enzymatically amplified genomic sequences.

6. Errors in the polymerase chain reaction.

7. Diagnosis of alpha 1-antitrypsin deficiency by enzymatic amplification of human genomic DNA and direct sequencing of polymerase chain reaction products.

1. Theoretical consideration of amplification strategies.

Review 2. [Polymerase chain reaction: an overview].

3. Rapid, sequence-specific detection of unpurified PCR amplicons via a reusable, electrochemical sensor.

4. A quantitative approach for polymerase chain reactions based on a hidden Markov model.

5. A copia-like element in Pisum demonstrates the uses of dispersed repeated sequences in genetic analysis.

6. Diagnosis of haemophilia B using the polymerase chain reaction.

Review 7. Application of the polymerase chain reaction to the diagnosis of human genetic disease.

8. Theoretical uncertainty of measurements using quantitative polymerase chain reaction.

9. A coalescent approach to the polymerase chain reaction.

10. An infectious cDNA copy of the genome of a non-cardiovirulent coxsackievirus B3 strain: its complete sequence analysis and comparison to the genomes of cardiovirulent coxsackieviruses.