Ting-Yu Yen1, Yun-Ju Sung2, Hsiao-Chuan Lin1, Ching-Tien Peng1, Ni Tien2, Kao-Pin Hwang1, Jang-Jih Lu3. 1. Department of Pediatrics, Children's Hospital, China Medical University Hospital, Taichung, Taiwan; School of Medicine, China Medical University, Taichung, Taiwan. 2. Department of Laboratory Medicine, China Medical University Hospital, Taichung, Taiwan. 3. Department of Medical Research, China Medical University Hospital, Taichung, Taiwan; Department of Laboratory Medicine, Chang Gung Memorial Hospital at Linkou, Taoyuan, Taiwan; Department of Medical Biotechnology and Laboratory Science, Chang Gung University, Kwei-Shan, Taoyuan, Taiwan. Electronic address: janglu45@gmail.com.
Abstract
BACKGROUND: Staphylococcus lugdunensis has emerged as a key pathogen for clinical infection. It is sensitive to most antistaphylococcal agents, but it is increasingly resistant to β-lactam antibiotics. Oxacillin-resistant S. lugdunensis isolates carrying the mecA gene pose a major concern for therapy failure. METHODS: To assess the epidemiology and presence of mecA in S. lugdunensis, we gauged the prevalence and antibiotic resistance of S. lugdunensis in clinical specimens by using multiplex polymerase chain reaction (PCR) and pulsed-field gel electrophoresis. RESULTS: Thirty S. lugdunensis isolates were collected and examined between October 2009 and December 2010. The resistance to penicillin (87%) and oxacillin (20%) was noted. All oxacillin-resistant isolates (6/30) had type V or VT SCCmec. Most (67%, 4/6) isolates carried SCCmec type V. These organisms caused invasive infections such as peritonitis, osteomyelitis, and septic arthritis. Pulsed-field gel electrophoresis analyses showed most (83%, 5/6) isolates carrying mecA were pulsotype D with high similarity (93.8%). CONCLUSIONS: The findings suggest oxacillin-resistant S. lugdunensis carrying SCCmec type V is emerging in central Taiwan.
BACKGROUND:Staphylococcus lugdunensis has emerged as a key pathogen for clinical infection. It is sensitive to most antistaphylococcal agents, but it is increasingly resistant to β-lactam antibiotics. Oxacillin-resistant S. lugdunensis isolates carrying the mecA gene pose a major concern for therapy failure. METHODS: To assess the epidemiology and presence of mecA in S. lugdunensis, we gauged the prevalence and antibiotic resistance of S. lugdunensis in clinical specimens by using multiplex polymerase chain reaction (PCR) and pulsed-field gel electrophoresis. RESULTS: Thirty S. lugdunensis isolates were collected and examined between October 2009 and December 2010. The resistance to penicillin (87%) and oxacillin (20%) was noted. All oxacillin-resistant isolates (6/30) had type V or VT SCCmec. Most (67%, 4/6) isolates carried SCCmec type V. These organisms caused invasive infections such as peritonitis, osteomyelitis, and septic arthritis. Pulsed-field gel electrophoresis analyses showed most (83%, 5/6) isolates carrying mecA were pulsotype D with high similarity (93.8%). CONCLUSIONS: The findings suggest oxacillin-resistant S. lugdunensis carrying SCCmec type V is emerging in central Taiwan.
Authors: Rosa Fernández-Fernández; Carmen Lozano; Laura Ruiz-Ripa; Beatriz Robredo; José Manuel Azcona-Gutiérrez; Carla Andrea Alonso; Carmen Aspiroz; Myriam Zarazaga; Carmen Torres Journal: Microorganisms Date: 2022-07-22