Literature DB >> 2564775

13C-n.m.r. study of citrate metabolism in rabbit renal proximal-tubule cells.

A W Jans1, C Winkel, L Buitenhuis, J Lugtenburg.   

Abstract

The metabolism of a 13C-labelled substrate, [3-13C]citrate, was monitored in rabbit renal proximal-tubule cells by 13C n.m.r. The relative enrichments of label in glutamate, glutamine and alpha beta-glucose allowed a calculation of the rate of the glutamate dehydrogenase flux relative to the flux via phosphoenolpyruvate carboxykinase. A ratio of 1.2 +/- 0.05 was found. Under steady-state conditions of active gluconeogenesis, the ratio of flux through pyruvate kinase to the gluconeogenic rate was 0.97 +/- 0.03.

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Year:  1989        PMID： 2564775      PMCID： PMC1135597          DOI： 10.1042/bj2570425

Source DB:  PubMed          Journal:  Biochem J        ISSN： 0264-6021            Impact factor:   3.857


  8 in total

1.  13C and 31P NMR study of gluconeogenesis: utilization of 13C-labeled substrates by perfused liver from streptozotocin-diabetic and untreated rats.

Authors:  S M Cohen
Journal:  Biochemistry       Date:  1987-01-27       Impact factor: 3.162

2.  Natural-abundance 13C NMR of brain.

Authors:  M Bárány; C Arús; Y C Chang
Journal:  Magn Reson Med       Date:  1985-06       Impact factor: 4.668

3.  Effects of insulin on perfused liver from streptozotocin-diabetic and untreated rats: 13C NMR assay of pyruvate kinase flux.

Authors:  S M Cohen
Journal:  Biochemistry       Date:  1987-01-27       Impact factor: 3.162

4.  13C-NMR study of glycerol metabolism in rabbit renal cells of proximal convoluted tubules.

Authors:  A W Jans; R Willem
Journal:  Eur J Biochem       Date:  1988-05-16

5.  Mathematical analysis of isotope labeling in the citric acid cycle with applications to 13C NMR studies in perfused rat hearts.

Authors:  E M Chance; S H Seeholzer; K Kobayashi; J R Williamson
Journal:  J Biol Chem       Date:  1983-11-25       Impact factor: 5.157

6.  Improved renal cortical tubule suspension: spectrophotometric study of O2 delivery.

Authors:  R S Balaban; S P Soltoff; J M Storey; L J Mandel
Journal:  Am J Physiol       Date:  1980-01

7.  13C NMR study of effects of fasting and diabetes on the metabolism of pyruvate in the tricarboxylic acid cycle and the utilization of pyruvate and ethanol in lipogenesis in perfused rat liver.

Authors:  S M Cohen
Journal:  Biochemistry       Date:  1987-01-27       Impact factor: 3.162

8.  Carbon flux through citric acid cycle pathways in perfused heart by 13C NMR spectroscopy.

Authors:  C R Malloy; A D Sherry; F M Jeffrey
Journal:  FEBS Lett       Date:  1987-02-09       Impact factor: 4.124
  8 in total
  2 in total

1.  An n.m.r. study of the tricarboxylic acid cycle.

Authors:  J Katz
Journal:  Biochem J       Date:  1989-11-01       Impact factor: 3.857

2.  The fate of [3-13C]citrate in renal cells monitored by 13C n.m.r.

Authors:  A W Jans
Journal:  Biochem J       Date:  1989-11-01       Impact factor: 3.857
  2 in total

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