Özlem Özer Yücel1, Ezel Berker2, Lütfiye Mesci3, Kenan Eratalay2, Eser Tepe4, İlhan Tezcan5. 1. Department of Oral Pathology, Faculty of Dentistry, University of Gazi, Ankara, Turkey. Electronic address: ozlemozertr@gmail.com. 2. Department of Periodontology, Faculty of Dentistry, Hacettepe University, Ankara, Turkey. 3. Genetic Technologies, Diagnostics and Therapies INC., İstanbul, Turkey. 4. T.C. Ministry of Health, Tepebaşı Mouth and Tooth Health Hospital, Ankara, Turkey. 5. Hacettepe University, Medical Faculty, Children's Hospital, Immunology Unit, Ankara, Turkey.
Abstract
OBJECTIVES: The purpose of this study was to determine the differences in the distribution of TNF-α (-308) gene polymorphism among aggressive periodontitis, chronic periodontitis and periodontally healthy individuals and also to investigate whether this polymorphism is associated with gingival crevicular fluid TNF-α levels and periodontal disease severity. MATERIAL AND METHODS: A total of 93 individuals were enrolled in the study including 38 aggressive periodontitis, 29 chronic periodontitis patients, and 26 healthy controls. Single nucleotide polymorphism at TNF-α (-308) is analyzed by PCR-RFLP method. Gingival crevicular fluid samples were analyzed for TNF-α, using ELISA. RESULTS: The distribution of genotypes and allele frequencies for TNF-α (-308) were similar among the groups. After stratification of patients with respect to attachment level, aggressive periodontitis patients with clinical attachment level ⩾4mm was observed to have a higher frequency of TNF-α (-308) allele 2 compared to the chronic periodontitis patients with clinical attachment level ⩾4mm. No significant differences were found between the TNF-α levels of the different genotypes in spite of an insignificant increase in patient groups carrying TNF-α (-308) allele 2. CONCLUSION: The results of this study revealed an association between TNF-α (-308) allele 2 frequency and aggressive periodontitis patients with clinical attachment level ⩾4mm in the population studied.
OBJECTIVES: The purpose of this study was to determine the differences in the distribution of TNF-α (-308) gene polymorphism among aggressive periodontitis, chronic periodontitis and periodontally healthy individuals and also to investigate whether this polymorphism is associated with gingival crevicular fluid TNF-α levels and periodontal disease severity. MATERIAL AND METHODS: A total of 93 individuals were enrolled in the study including 38 aggressive periodontitis, 29 chronic periodontitispatients, and 26 healthy controls. Single nucleotide polymorphism at TNF-α (-308) is analyzed by PCR-RFLP method. Gingival crevicular fluid samples were analyzed for TNF-α, using ELISA. RESULTS: The distribution of genotypes and allele frequencies for TNF-α (-308) were similar among the groups. After stratification of patients with respect to attachment level, aggressive periodontitispatients with clinical attachment level ⩾4mm was observed to have a higher frequency of TNF-α (-308) allele 2 compared to the chronic periodontitispatients with clinical attachment level ⩾4mm. No significant differences were found between the TNF-α levels of the different genotypes in spite of an insignificant increase in patient groups carrying TNF-α (-308) allele 2. CONCLUSION: The results of this study revealed an association between TNF-α (-308) allele 2 frequency and aggressive periodontitispatients with clinical attachment level ⩾4mm in the population studied.
Authors: Milena Kostic; Marko Igic; Tatjana Jevtovic Stoimenov; Ana Pejcic; Jana Pesic Stankovic Journal: Med Princ Pract Date: 2019-03-08 Impact factor: 1.927
Authors: Víctor M Martínez-Aguilar; Bertha A Carrillo-Ávila; Eduardo A Sauri-Esquivel; Eugenia Guzmán-Marín; Matilde Jiménez-Coello; Diana María Escobar-García; Amaury Pozos-Guillén Journal: Biomed Res Int Date: 2019-07-04 Impact factor: 3.411