Guilherme F Silva1, Mário Tanomaru-Filho1, Maria I B Bernardi2, Juliane M Guerreiro-Tanomaru1, Paulo S Cerri3,4. 1. Department of Restorative Dentistry, UNESP-Univ. Estadual Paulista, Dental School, Araraquara, SP, Brazil. 2. Physics Institute of São Carlos, Grupo Crescimento de Cristais e Materiais Cerâmicos, University of São Paulo-USP, São Carlos, SP, Brazil. 3. Laboratory of Histology and Embryology, UNESP-Univ. Estadual Paulista, Dental School, Araraquara, SP, Brazil. pcerri@foar.unesp.br. 4. Department of Morphology-Laboratory of Histology and Embryology, UNESP-Univ Estadual Paulista, Dental School, Rua Humaitá, 1680, Centro, CEP, 14801-903, Araraquara, SP, Brazil. pcerri@foar.unesp.br.
Abstract
OBJECTIVES: The physicochemical properties and the tissue reaction promoted by microparticulated or nanoparticulated niobium pentoxide (Nb2O5) added to calcium silicate-based cement (CS), compared to MTA-Angelus™, were evaluated. MATERIALS AND METHODS: Materials were submitted to the tests of radiopacity, setting time, pH, and calcium ion release. Polyethylene tubes filled with the materials were implanted into rats subcutaneously. After 7, 15, 30, and 60 days, the specimens were fixed and embedded in paraffin. Hematoxylin & eosin (H&E)-stained sections were used to compute the number of inflammatory cells (IC). Interleukin-6 (IL-6) detection was performed, and the number of immunolabeled cells was obtained; von Kossa method was also carried out. Data were subjected to ANOVA and Tukey test (p ≤ 0.05). RESULTS: Nb2O5micro and Nb2O5nano provided to the CS radiopacity values (3.52 and 3.75 mm Al, respectively) superior to the minimum recommended. Groups containing Nb2O5 presented initial setting time significantly superior than mineral trioxide aggregate (MTA). All materials presented an alkaline pH and released calcium ions. The number of IC and IL-6 immunolabeled cells in the CS + Nb2O5 groups was significantly reduced in comparison to MTA in all periods. von Kossa-positive structures were observed adjacent to implanted materials in all periods. CONCLUSIONS: The addition of Nb2O5 to the CS resulted in a material biocompatible and with adequate characteristics regarding radiopacity and final setting time and provides an alkaline pH to the environment. Furthermore, the particle size did not significantly affect the physicochemical and biological properties of the calcium silicate-based cement. CLINICAL RELEVANCE: Niobium pentoxide can be used as radiopacifier for the development of calcium silicate-based materials.
OBJECTIVES: The physicochemical properties and the tissue reaction promoted by microparticulated or nanoparticulated niobium pentoxide (Nb2O5) added to calcium silicate-based cement (CS), compared to MTA-Angelus™, were evaluated. MATERIALS AND METHODS: Materials were submitted to the tests of radiopacity, setting time, pH, and calcium ion release. Polyethylene tubes filled with the materials were implanted into rats subcutaneously. After 7, 15, 30, and 60 days, the specimens were fixed and embedded in paraffin. Hematoxylin & eosin (H&E)-stained sections were used to compute the number of inflammatory cells (IC). Interleukin-6 (IL-6) detection was performed, and the number of immunolabeled cells was obtained; von Kossa method was also carried out. Data were subjected to ANOVA and Tukey test (p ≤ 0.05). RESULTS: Nb2O5micro and Nb2O5nano provided to the CS radiopacity values (3.52 and 3.75 mm Al, respectively) superior to the minimum recommended. Groups containing Nb2O5 presented initial setting time significantly superior than mineral trioxide aggregate (MTA). All materials presented an alkaline pH and released calcium ions. The number of IC and IL-6 immunolabeled cells in the CS + Nb2O5 groups was significantly reduced in comparison to MTA in all periods. von Kossa-positive structures were observed adjacent to implanted materials in all periods. CONCLUSIONS: The addition of Nb2O5 to the CS resulted in a material biocompatible and with adequate characteristics regarding radiopacity and final setting time and provides an alkaline pH to the environment. Furthermore, the particle size did not significantly affect the physicochemical and biological properties of the calcium silicate-based cement. CLINICAL RELEVANCE: Niobium pentoxide can be used as radiopacifier for the development of calcium silicate-based materials.
Authors: Naiana Viana Viola; Juliane Maria Guerreiro-Tanomaru; Guilherme Ferreira da Silva; Estela Sasso-Cerri; Mario Tanomaru-Filho; Paulo Sérgio Cerri Journal: J Biomed Mater Res B Appl Biomater Date: 2012-07-23 Impact factor: 3.368
Authors: Maria Giovanna Gandolfi; Gabriela Ciapetti; Paola Taddei; Francesca Perut; Anna Tinti; Marcio Vivan Cardoso; Bart Van Meerbeek; Carlo Prati Journal: Dent Mater Date: 2010-07-23 Impact factor: 5.304
Authors: V C B Leitune; A Takimi; F M Collares; P D Santos; C Provenzi; C P Bergmann; S M W Samuel Journal: Int Endod J Date: 2012-08-11 Impact factor: 5.264
Authors: Guilherme F Silva; Roberta Bosso; Rafael V Ferino; Mário Tanomaru-Filho; Maria I B Bernardi; Juliane M Guerreiro-Tanomaru; Paulo S Cerri Journal: J Biomed Mater Res A Date: 2014-02-20 Impact factor: 4.396
Authors: Emily V F da Silva; Marcelo C Goiato; Sandro B Bitencourt; Victor G B Brito; Aline S Takamyia; Paulo A Penitente; Sandra H P de Oliveira; Daniela M Dos Santos Journal: Sultan Qaboos Univ Med J Date: 2022-05-26