| Literature DB >> 2564315 |
J Minshull1, J J Blow, T Hunt.
Abstract
The cyclins are a family of proteins encoded by maternal mRNA. Cyclin polypeptides accumulate during interphase and are destroyed during mitosis at about the time of entry into anaphase. We show here that Xenopus oocytes contain mRNAs encoding two cyclins that are major translation products in a cell-free extract from activated eggs. Cutting these mRNAs with antisense oligonucleotides and endogenous RNAase H blocks entry into mitosis in a cell-free egg extract. The extracts can enter mitosis if either of the cyclin mRNAs is left intact. We conclude that the synthesis of these cyclins is necessary for mitotic cell cycles in cleaving Xenopus embryos.Entities:
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Year: 1989 PMID： 2564315 DOI： 10.1016/0092-8674(89)90628-4
Source DB: PubMed Journal: Cell ISSN： 0092-8674 Impact factor: 41.582