Literature DB >> 25640819

Teratoma formation: a tool for monitoring pluripotency in stem cell research.

Raman V Nelakanti1,2,3, Nigel G Kooreman1,2,3,4, Joseph C Wu1,2,3.   

Abstract

This unit describes protocols for evaluating the pluripotency of embryonic and induced pluripotent stem cells using a teratoma formation assay. Cells are prepared for injection and transplanted into immunodeficient mice at the gastrocnemius muscle, a site well suited for teratoma growth and surgical access. Teratomas that form from the cell transplants are explanted, fixed in paraformaldehyde, and embedded in paraffin. These preserved samples are sectioned, stained, and analyzed. Pluripotency of a cell line is confirmed by whether the teratoma contains tissues derived from each of the embryonic germ layers: endoderm, mesoderm, and ectoderm. Alternatively, explanted and fixed teratomas can be cryopreserved for immunohistochemistry, which allows for more detailed identification of specific tissue types present in the samples.
Copyright © 2015 John Wiley & Sons, Inc.

Entities:  

Keywords:  embryonic stem cell; induced pluripotent stem cell; pluripotency; pluripotent stem cell; teratoma; tumorigenicity

Mesh：

Year:  2015        PMID： 25640819      PMCID： PMC4402211          DOI： 10.1002/9780470151808.sc04a08s32

Source DB:  PubMed          Journal:  Curr Protoc Stem Cell Biol        ISSN： 1938-8969


  49 in total

1.  A call to standardize teratoma assays used to define human pluripotent cell lines.

Authors:  Franz-Josef Müller; Johanna Goldmann; Peter Löser; Jeanne F Loring
Journal:  Cell Stem Cell       Date:  2010-05-07       Impact factor: 24.633

2.  Monitoring differentiation of human embryonic stem cells using real-time PCR.

Authors:  Karin Noaksson; Neven Zoric; Xianmin Zeng; Mahendra S Rao; Johan Hyllner; Henrik Semb; Mikael Kubista; Peter Sartipy
Journal:  Stem Cells       Date:  2005-08-04       Impact factor: 6.277

3.  Pluripotency governed by Sox2 via regulation of Oct3/4 expression in mouse embryonic stem cells.

Authors:  Shinji Masui; Yuhki Nakatake; Yayoi Toyooka; Daisuke Shimosato; Rika Yagi; Kazue Takahashi; Hitoshi Okochi; Akihiko Okuda; Ryo Matoba; Alexei A Sharov; Minoru S H Ko; Hitoshi Niwa
Journal:  Nat Cell Biol       Date:  2007-05-21       Impact factor: 28.824

4.  Bilateral ovarian teratomas in a mouse.

Authors:  D W FAWCETT
Journal:  Cancer Res       Date:  1950-11       Impact factor: 12.701

5.  Characterization of a new NIH-registered variant human embryonic stem cell line, BG01V: a tool for human embryonic stem cell research.

Authors:  Todd W Plaia; Richard Josephson; Ying Liu; Xianmin Zeng; Carol Ording; Arazdordi Toumadje; Sandii N Brimble; Eric S Sherrer; Elizabeth W Uhl; William J Freed; Thomas C Schulz; Anirban Maitra; Mahendra S Rao; Jonathan M Auerbach
Journal:  Stem Cells       Date:  2005-11-17       Impact factor: 6.277

6.  Dynamic changes in the copy number of pluripotency and cell proliferation genes in human ESCs and iPSCs during reprogramming and time in culture.

Authors:  Louise C Laurent; Igor Ulitsky; Ileana Slavin; Ha Tran; Andrew Schork; Robert Morey; Candace Lynch; Julie V Harness; Sunray Lee; Maria J Barrero; Sherman Ku; Marina Martynova; Ruslan Semechkin; Vasiliy Galat; Joel Gottesfeld; Juan Carlos Izpisua Belmonte; Chuck Murry; Hans S Keirstead; Hyun-Sook Park; Uli Schmidt; Andrew L Laslett; Franz-Josef Muller; Caroline M Nievergelt; Ron Shamir; Jeanne F Loring
Journal:  Cell Stem Cell       Date:  2011-01-07       Impact factor: 24.633

7.  Embryonic stem cell lines derived from human blastocysts.

Authors:  J A Thomson; J Itskovitz-Eldor; S S Shapiro; M A Waknitz; J J Swiergiel; V S Marshall; J M Jones
Journal:  Science       Date:  1998-11-06       Impact factor: 47.728

8.  Alterations of proliferative and differentiation potentials of human embryonic stem cells during long-term culture.

Authors:  Yong Bin Park; Yoon Young Kim; Sun Kyung Oh; Sun Gan Chung; Seung Yup Ku; Seok Hyun Kim; Young Min Choi; Shin Yong Moon
Journal:  Exp Mol Med       Date:  2008-02-29       Impact factor: 8.718

9.  The transcriptional and epigenomic foundations of ground state pluripotency.

Authors:  Hendrik Marks; Tüzer Kalkan; Roberta Menafra; Sergey Denissov; Kenneth Jones; Helmut Hofemeister; Jennifer Nichols; Andrea Kranz; A Francis Stewart; Austin Smith; Hendrik G Stunnenberg
Journal:  Cell       Date:  2012-04-27       Impact factor: 41.582

10.  Extended passaging increases the efficiency of neural differentiation from induced pluripotent stem cells.

Authors:  Karl R Koehler; Philippe Tropel; Jonathan W Theile; Takako Kondo; Theodore R Cummins; Stéphane Viville; Eri Hashino
Journal:  BMC Neurosci       Date:  2011-08-10       Impact factor: 3.288
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  32 in total

Review 1.  Progressing a human embryonic stem-cell-based regenerative medicine therapy towards the clinic.

Authors:  Paul Whiting; Julie Kerby; Peter Coffey; Lyndon da Cruz; Ruth McKernan
Journal:  Philos Trans R Soc Lond B Biol Sci       Date:  2015-10-19       Impact factor: 6.237

2.  An intrinsic, label-free signal for identifying stem cell-derived cardiomyocyte subtype.

Authors:  Che-Wei Chang; Hillary K J Kao; Sergey Yechikov; Deborah K Lieu; James W Chan
Journal:  Stem Cells       Date:  2019-12-09       Impact factor: 6.277

3.  Tracking and Predicting Human Somatic Cell Reprogramming Using Nuclear Characteristics.

Authors:  Kaivalya Molugu; Ty Harkness; Jared Carlson-Stevermer; Ryan Prestil; Nicole J Piscopo; Stephanie K Seymour; Gavin T Knight; Randolph S Ashton; Krishanu Saha
Journal:  Biophys J       Date:  2019-10-22       Impact factor: 4.033

4.  HoxBlinc RNA Recruits Set1/MLL Complexes to Activate Hox Gene Expression Patterns and Mesoderm Lineage Development.

Authors:  Changwang Deng; Ying Li; Lei Zhou; Joonseok Cho; Bhavita Patel; Naohiro Terada; Yangqiu Li; Jörg Bungert; Yi Qiu; Suming Huang
Journal:  Cell Rep       Date:  2015-12-24       Impact factor: 9.423

Review 5.  Stem cells on regenerative and reproductive science in domestic animals.

Authors:  Naira Caroline Godoy Pieri; Aline Fernanda de Souza; Ramon Cesar Botigelli; Lucas Simões Machado; Carlos Eduardo Ambrosio; Daniele Dos Santos Martins; André Furugen Cesar de Andrade; Flavio Vieira Meirelles; Poul Hyttel; Fabiana Fernandes Bressan
Journal:  Vet Res Commun       Date:  2019-01-17       Impact factor: 2.459

6.  Anti-fibrotic effect of a selective estrogen receptor modulator in systemic sclerosis.

Authors:  Yena Kim; Yoojun Nam; Yeri Alice Rim; Ji Hyeon Ju
Journal:  Stem Cell Res Ther       Date:  2022-07-15       Impact factor: 8.079

7.  Pluripotent stem cells as a source of osteoblasts for bone tissue regeneration.

Authors:  Hui Zhu; Takaharu Kimura; Srilatha Swami; Joy Y Wu
Journal:  Biomaterials       Date:  2018-02-05       Impact factor: 12.479

8.  Autologous iPSC-Based Vaccines Elicit Anti-tumor Responses In Vivo.

Authors:  Nigel G Kooreman; Youngkyun Kim; Patricia E de Almeida; Vittavat Termglinchan; Sebastian Diecke; Ning-Yi Shao; Tzu-Tang Wei; Hyoju Yi; Devaveena Dey; Raman Nelakanti; Thomas P Brouwer; David T Paik; Idit Sagiv-Barfi; Arnold Han; Paul H A Quax; Jaap F Hamming; Ronald Levy; Mark M Davis; Joseph C Wu
Journal:  Cell Stem Cell       Date:  2018-02-15       Impact factor: 24.633

9.  OpenTein: a database of digital whole-slide images of stem cell-derived teratomas.

Authors:  Sung-Joon Park; Yusuke Komiyama; Hirofumi Suemori; Akihiro Umezawa; Kenta Nakai
Journal:  Nucleic Acids Res       Date:  2015-10-22       Impact factor: 16.971

10.  Role of circadian gene Clock during differentiation of mouse pluripotent stem cells.

Authors:  Chao Lu; Yang Yang; Ran Zhao; Bingxuan Hua; Chen Xu; Zuoqin Yan; Ning Sun; Ruizhe Qian
Journal:  Protein Cell       Date:  2016-09-23       Impact factor: 14.870
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