| Literature DB >> 25636474 |
Rebecca D Dodd1, Leonor Añó, Jordan M Blum, Zhizhong Li, David Van Mater, David G Kirsch.
Abstract
We discuss the generation of primary soft tissue sarcomas in mice using the Cre-loxP system to activate conditional mutations in oncogenic Kras and the tumor suppressor p53 (LSL-Kras(G12D/+); p53(flox/flox)). Sarcomas can be generated either by adenoviral delivery of Cre recombinase, activation of transgenic Cre recombinase with tamoxifen, or through transplantation of isolated satellite cells with Cre activation in vitro. Various applications of these models are discussed, including anticancer therapies, metastasis, in vivo imaging, and genetic requirements for tumorigenesis.Entities:
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Year: 2015 PMID： 25636474 PMCID： PMC5612825 DOI： 10.1007/978-1-4939-2297-0_13
Source DB: PubMed Journal: Methods Mol Biol ISSN： 1064-3745