Jingsong Xu1, Yu Wu2, Guifang Wang3, Yanghua Qin4, Li Zhu4, Gusheng Tang5, Qian Shen4. 1. Department of Pulmonary Medicine, 94th Hospital of The Chinese People's Liberation Army Nanchang, China ; Department of Laboratory Medicine, Changhai Hospital, Second Military Medical University Shanghai, China. 2. Department of Laboratory Medicine, Changhai Hospital, Second Military Medical University Shanghai, China ; Department of Laboratory Medicine, 94th Hospital of The Chinese People's Liberation Army Nanchang, China. 3. Department of Pulmonary Medicine, Huashan Hospital, Fudan University Shanghai, China. 4. Department of Laboratory Medicine, Changhai Hospital, Second Military Medical University Shanghai, China. 5. Institute of Hematology, Changhai Hospital, Second Military Medical University Shanghai, China.
Abstract
OBJECTIVE: This study aimed to investigate the role of inducible costimulatory molecule (ICOS) pathway in the rejection reaction of mice undergoing allogeneic tracheal transplantation. METHODS: The bronchus was separated from wide-type (WT) BalB/c mice and transplanted into WT BalB/c mice, C57 mice and icos(-/-) mice to prepare the obliterative bronchiolitis (OB) animal model. The transplanted bronchus was pathologically examined; flow cytometry was done to detect the T cell subsets and activity of the bronchus and spleen of recipient mice. RESULTS: 21 d after transplantation, evident rejection reaction was observed and the proportion of Th2 and Th17 cells increased significantly in the bronchus and spleen in C57 mice receiving allogeneic tracheal transplantation when compared with mice with autologous transplantation, but the proportion of Treg cells was comparable between them. When compared with WT BalB/c mice, the proportion of Th2, Th17 and Treg cells reduced markedly and rejection reaction was attenuated in icos(-/-) mice receiving tracheal transplantation, although rejection reaction was still noted. CONCLUSION: icos knockout may delay the rejection reaction after tracheal transplantation, which might be ascribed to the imbalance among Th2, Th17 and Treg cells.
OBJECTIVE: This study aimed to investigate the role of inducible costimulatory molecule (ICOS) pathway in the rejection reaction of mice undergoing allogeneic tracheal transplantation. METHODS: The bronchus was separated from wide-type (WT) BalB/c mice and transplanted into WT BalB/c mice, C57 mice and icos(-/-) mice to prepare the obliterative bronchiolitis (OB) animal model. The transplanted bronchus was pathologically examined; flow cytometry was done to detect the T cell subsets and activity of the bronchus and spleen of recipient mice. RESULTS: 21 d after transplantation, evident rejection reaction was observed and the proportion of Th2 and Th17 cells increased significantly in the bronchus and spleen in C57 mice receiving allogeneic tracheal transplantation when compared with mice with autologous transplantation, but the proportion of Treg cells was comparable between them. When compared with WT BalB/c mice, the proportion of Th2, Th17 and Treg cells reduced markedly and rejection reaction was attenuated in icos(-/-) mice receiving tracheal transplantation, although rejection reaction was still noted. CONCLUSION: icos knockout may delay the rejection reaction after tracheal transplantation, which might be ascribed to the imbalance among Th2, Th17 and Treg cells.
Authors: Jacob R Gillen; Yunge Zhao; David A Harris; Damien J LaPar; Irving L Kron; Christine L Lau Journal: Ann Thorac Surg Date: 2013-06-24 Impact factor: 4.330
Authors: M M Imanguli; E W Cowen; J Rose; S Dhamala; W Swaim; S Lafond; B Yagi; R E Gress; S Z Pavletic; F T Hakim Journal: Leukemia Date: 2014-02-28 Impact factor: 11.528
Authors: Alex KleinJan; Monique A M Willart; Harmjan Kuipers; Anthony J Coyle; Henk C Hoogsteden; Bart N Lambrecht Journal: Transplantation Date: 2008-11-27 Impact factor: 4.939
Authors: Ryan Flynn; Jing Du; Rachelle G Veenstra; Dawn K Reichenbach; Angela Panoskaltsis-Mortari; Patricia A Taylor; Gordon J Freeman; Jonathan S Serody; William J Murphy; David H Munn; Stefanie Sarantopoulos; Leo Luznik; Ivan Maillard; John Koreth; Corey Cutler; Robert J Soiffer; Joseph H Antin; Jerome Ritz; Jason A Dubovsky; John C Byrd; Kelli P MacDonald; Geoff R Hill; Bruce R Blazar Journal: Blood Date: 2014-05-12 Impact factor: 22.113
Authors: Philippe H Lemaître; Benoît Vokaer; Louis-Marie Charbonnier; Yoichiro Iwakura; Marc Estenne; Michel Goldman; Oberdan Leo; Myriam Remmelink; Alain Le Moine Journal: PLoS One Date: 2013-07-30 Impact factor: 3.240
Authors: Katarzyna A Darlak; Ying Wang; Jian-Ming Li; Wayne Ac Harris; Cynthia R Giver; Chunzi Huang; Edmund K Waller Journal: J Hematol Oncol Date: 2014-02-28 Impact factor: 17.388