Literature DB >> 25628018

In vitro translation in a hybrid cell free lysate with exogenous cellular ribosomes.

Baptiste Panthu1, Didier Décimo1, Laurent Balvay1, Théophile Ohlmann1.   

Abstract

Cell free protein synthesis systems (CFPS) have been widely used to express proteins and to explore the pathways of gene expression. In the present manuscript, we describe the design of a novel adaptable hybrid in vitro translation system which is assembled with ribosomes isolated from many different origins. We first show that this hybrid system exhibits all important features such as efficiency, sensitivity, reproducibility and the ability to translate specialized mRNAs in less than 1 h. In addition, the unique design of this cell free assay makes it highly adaptable to utilize ribosomes isolated from many different organs, tissues or cell types.

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Year:  2015        PMID： 25628018     DOI： 10.1042/BJ20141498

Source DB:  PubMed          Journal:  Biochem J        ISSN： 0264-6021            Impact factor:   3.857


  10 in total

1.  Epstein-Barr Virus Protein EB2 Stimulates Translation Initiation of mRNAs through Direct Interactions with both Poly(A)-Binding Protein and Eukaryotic Initiation Factor 4G.

Authors:  Fabrice Mure; Baptiste Panthu; Isabelle Zanella-Cléon; Frédéric Delolme; Evelyne Manet; Théophile Ohlmann; Henri Gruffat
Journal:  J Virol       Date:  2018-01-17       Impact factor: 5.103

2.  Unlike for cellular mRNAs and other viral internal ribosome entry sites (IRESs), the eIF3 subunit e is not required for the translational activity of the HCV IRES.

Authors:  Baptiste Panthu; Solène Denolly; Cendrine Faivre-Moskalenko; Théophile Ohlmann; François-Loïc Cosset; Pierre Jalinot
Journal:  J Biol Chem       Date:  2020-01-12       Impact factor: 5.157

3.  METTL18-mediated histidine methylation of RPL3 modulates translation elongation for proteostasis maintenance.

Authors:  Eriko Matsuura-Suzuki; Tadahiro Shimazu; Mari Takahashi; Kaoru Kotoshiba; Takehiro Suzuki; Kazuhiro Kashiwagi; Yoshihiro Sohtome; Mai Akakabe; Mikiko Sodeoka; Naoshi Dohmae; Takuhiro Ito; Yoichi Shinkai; Shintaro Iwasaki
Journal:  Elife       Date:  2022-06-08       Impact factor: 8.713

4.  A reconstituted cell-free assay for the evaluation of the intrinsic activity of purified human ribosomes.

Authors:  Marianna Penzo; Domenica Carnicelli; Lorenzo Montanaro; Maurizio Brigotti
Journal:  Nat Protoc       Date:  2016-06-23       Impact factor: 13.491

Review 5.  Emerging Role of Eukaryote Ribosomes in Translational Control.

Authors:  Nicole Dalla Venezia; Anne Vincent; Virginie Marcel; Frédéric Catez; Jean-Jacques Diaz
Journal:  Int J Mol Sci       Date:  2019-03-11       Impact factor: 5.923

6.  Alteration of ribosome function upon 5-fluorouracil treatment favors cancer cell drug-tolerance.

Authors:  Gabriel Therizols; Zeina Bash-Imam; Baptiste Panthu; Christelle Machon; Anne Vincent; Julie Ripoll; Sophie Nait-Slimane; Mounira Chalabi-Dchar; Angéline Gaucherot; Maxime Garcia; Florian Laforêts; Virginie Marcel; Jihane Boubaker-Vitre; Marie-Ambre Monet; Céline Bouclier; Christophe Vanbelle; Guillaume Souahlia; Elise Berthel; Marie Alexandra Albaret; Hichem C Mertani; Michel Prudhomme; Martin Bertrand; Alexandre David; Jean-Christophe Saurin; Philippe Bouvet; Eric Rivals; Théophile Ohlmann; Jérôme Guitton; Nicole Dalla Venezia; Julie Pannequin; Frédéric Catez; Jean-Jacques Diaz
Journal:  Nat Commun       Date:  2022-01-10       Impact factor: 17.694

7.  Development, validation, and application of the ribosome separation and reconstitution system for protein translation in vitro.

Authors:  Brandon M Trainor; Dimitri G Pestov; Natalia Shcherbik
Journal:  RNA       Date:  2021-08-27       Impact factor: 4.942

8.  Premature aging in mice with error-prone protein synthesis.

Authors:  Dimitri Shcherbakov; Martina Nigri; Rashid Akbergenov; Margarita Brilkova; Matilde Mantovani; Patricia Isnard Petit; Amandine Grimm; Agnieszka A Karol; Youjin Teo; Adrián Cortés Sanchón; Yadhu Kumar; Anne Eckert; Kader Thiam; Petra Seebeck; David P Wolfer; Erik C Böttger
Journal:  Sci Adv       Date:  2022-03-02       Impact factor: 14.136

9.  Evidence for rRNA 2'-O-methylation plasticity: Control of intrinsic translational capabilities of human ribosomes.

Authors:  Jenny Erales; Virginie Marchand; Baptiste Panthu; Sandra Gillot; Stéphane Belin; Sandra E Ghayad; Maxime Garcia; Florian Laforêts; Virginie Marcel; Agnès Baudin-Baillieu; Pierre Bertin; Yohann Couté; Annie Adrait; Mélanie Meyer; Gabriel Therizols; Marat Yusupov; Olivier Namy; Théophile Ohlmann; Yuri Motorin; Frédéric Catez; Jean-Jacques Diaz
Journal:  Proc Natl Acad Sci U S A       Date:  2017-11-20       Impact factor: 11.205

Review 10.  2'-O-Methylation of Ribosomal RNA: Towards an Epitranscriptomic Control of Translation?

Authors:  Piero Lo Monaco; Virginie Marcel; Jean-Jacques Diaz; Frédéric Catez
Journal:  Biomolecules       Date:  2018-10-03
  10 in total

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