Saisai Wang1, Gang Zheng2, Shousheng Tian3, Yan Zhang3, Lijuan Shen4, Yongchol Pak2, Yong Shen1, Jing Qian5. 1. Cancer Institute, Key Laboratory of Cancer Prevention and Intervention, China National Ministry of Education, the Second Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou, Zhejiang 310009, China; Department of Medical Microbiology and Parasitology, Research Center of Infection and Immunity, Zhejiang University School of Medicine, Hangzhou, Zhejiang 310058, China. 2. Department of Medical Microbiology and Parasitology, Research Center of Infection and Immunity, Zhejiang University School of Medicine, Hangzhou, Zhejiang 310058, China. 3. Engineering Technology Research Center of Glue of Traditional Medicine, Shandong Dongeejiao Co., Ltd, Shandong 252201, China. 4. Pharmaceutical Informatics Institute, College of Pharmaceutical Sciences, Zhejiang University, Hangzhou, Zhejiang 310058, China. 5. Department of Medical Microbiology and Parasitology, Research Center of Infection and Immunity, Zhejiang University School of Medicine, Hangzhou, Zhejiang 310058, China. Electronic address: jingqian@zju.edu.cn.
Abstract
AIMS: We aimed to investigate the effects of echinacoside (ECH) on hematopoietic function in 5-FU-induced bone marrow depression mice. MAIN METHODS: In vitro, after stimulation with ECH, the proliferation ability of bone marrow (BM) cells and bone marrow stromal cells (BMSCs) derived from myelosuppression mice were assessed by CCK8 assay and morphology, respectively. In vivo, 5-FU-induced myelosuppression or control mice were intragastrically administrated with either ECH at 15 mg/kg or the equal volume of normal saline daily for 12 days before BM cells were isolated for colony-forming cell assay. Meanwhile, BMSCs were cultured for 4 weeks before cells were observed for growth pattern, cell culture supernatants were collected for GM-CSF secretion by ELISA, and RNA of the cells were extracted for EPO and GM-CSF RT-PCR. BM cells or BMSCs stimulated with ECH for 24 h or 48 h were collected for protein extraction and Western blotting. KEY FINDINGS: ECH stimulated the growth of BM cells but not BMSCs derived from 5-FU treated mice. The intragastric administration of ECH in 5-FU treated mice could increase the number of total hematopoietic progenitor cells and GM progenitor cells to healthy control mice level, but not BFU progenitor cells. BMSCs from ECH treated myelosuppression mice grew more vigorously and expressed more GM-CSF, but not EPO. ECH activated the PI3K signaling pathway in 5-FU suppressed BM cells. SIGNIFICANCE: ECH could improve the hematopoietic function of bone marrow in 5-FU-induced myelosuppression mice. ECH can be considered as an alternative effective therapy for patients during chemotherapy or HSC transplantation.
AIMS: We aimed to investigate the effects of echinacoside (ECH) on hematopoietic function in 5-FU-induced bone marrow depressionmice. MAIN METHODS: In vitro, after stimulation with ECH, the proliferation ability of bone marrow (BM) cells and bone marrow stromal cells (BMSCs) derived from myelosuppressionmice were assessed by CCK8 assay and morphology, respectively. In vivo, 5-FU-induced myelosuppression or control mice were intragastrically administrated with either ECH at 15 mg/kg or the equal volume of normal saline daily for 12 days before BM cells were isolated for colony-forming cell assay. Meanwhile, BMSCs were cultured for 4 weeks before cells were observed for growth pattern, cell culture supernatants were collected for GM-CSF secretion by ELISA, and RNA of the cells were extracted for EPO and GM-CSF RT-PCR. BM cells or BMSCs stimulated with ECH for 24 h or 48 h were collected for protein extraction and Western blotting. KEY FINDINGS:ECH stimulated the growth of BM cells but not BMSCs derived from 5-FU treated mice. The intragastric administration of ECH in 5-FU treated mice could increase the number of total hematopoietic progenitor cells and GM progenitor cells to healthy control mice level, but not BFU progenitor cells. BMSCs from ECH treated myelosuppressionmice grew more vigorously and expressed more GM-CSF, but not EPO. ECH activated the PI3K signaling pathway in 5-FU suppressed BM cells. SIGNIFICANCE: ECH could improve the hematopoietic function of bone marrow in 5-FU-induced myelosuppressionmice. ECH can be considered as an alternative effective therapy for patients during chemotherapy or HSC transplantation.
Authors: Jinhee Kim; You Jin Lee; Young Ah Kim; Eun-Sang Cho; Eunna Huh; Ok-Sun Bang; No Soo Kim Journal: BMC Complement Altern Med Date: 2017-08-09 Impact factor: 3.659