Hanspeter Marti1, Chris Stalder2, Iveth J González3. 1. University of Basel, Basel, Switzerland; Medical and Diagnostics Department, Swiss Tropical and Public Health Institute (Swiss TPH), Basel, Switzerland. Electronic address: hanspeter.marti@unibas.ch. 2. University of Basel, Basel, Switzerland; Medical and Diagnostics Department, Swiss Tropical and Public Health Institute (Swiss TPH), Basel, Switzerland. 3. Foundation for Innovative New Diagnostics (FIND), Campus Biotech, Chemin des Mines 9, CH1211 Geneva, Switzerland.
Abstract
BACKGROUND: The diagnosis of acute malaria in non-endemic countries is still carried out largely by microscopic examination of thick and thin smears or rapid diagnostic tests. Low-density infections might be missed, however, but the more sensitive PCR is more expensive, complex and requires considerable more time. METHOD: We examined the suitability of a new loop-mediated isothermal DNA-amplification kit (LAMP) for malaria diagnosis in febrile returning travellers in comparison to qPCR and microscopic examination in a prospective study in a non-endemic setting at the Swiss TPH. RESULTS: Among 205 complete datasets, 43 samples were positive for malaria by microscopy, with Plasmodium falciparum (35 cases) being the most frequent species. All these samples were positive by both LAMP and qPCR, too. An additional 4 samples negative by microscopy were positive by both LAMP and qPCR. Three of these samples were follow-up samples taken after start of treatment in patients originally identified as positive by microscopy. CONCLUSIONS: The LAMP performed exactly as did the qPCR and is a very valuable diagnostic alternative with a potential of being used also in endemic settings.
BACKGROUND: The diagnosis of acute malaria in non-endemic countries is still carried out largely by microscopic examination of thick and thin smears or rapid diagnostic tests. Low-density infections might be missed, however, but the more sensitive PCR is more expensive, complex and requires considerable more time. METHOD: We examined the suitability of a new loop-mediated isothermal DNA-amplification kit (LAMP) for malaria diagnosis in febrile returning travellers in comparison to qPCR and microscopic examination in a prospective study in a non-endemic setting at the Swiss TPH. RESULTS: Among 205 complete datasets, 43 samples were positive for malaria by microscopy, with Plasmodium falciparum (35 cases) being the most frequent species. All these samples were positive by both LAMP and qPCR, too. An additional 4 samples negative by microscopy were positive by both LAMP and qPCR. Three of these samples were follow-up samples taken after start of treatment in patients originally identified as positive by microscopy. CONCLUSIONS: The LAMP performed exactly as did the qPCR and is a very valuable diagnostic alternative with a potential of being used also in endemic settings.
Authors: Spinello Antinori; Anna Lisa Ridolfo; Romualdo Grande; Laura Galimberti; Giacomo Casalini; Andrea Giacomelli; Laura Milazzo Journal: Infez Med Date: 2021-09-10
Authors: Avinash N Mukkala; Jason Kwan; Rachel Lau; David Harris; Dylan Kain; Andrea K Boggild Journal: Curr Infect Dis Rep Date: 2018-10-23 Impact factor: 3.725
Authors: Konstantinos Mitsakakis; Sebastian Hin; Pie Müller; Nadja Wipf; Edward Thomsen; Michael Coleman; Roland Zengerle; John Vontas; Konstantinos Mavridis Journal: Int J Environ Res Public Health Date: 2018-02-03 Impact factor: 3.390
Authors: James Cheaveau; Hong Nguyen; Barbara Chow; Dewdunee Marasinghe; Abu Naser Mohon; Hong Yuan; Gisele Viana; Donelly van Schalkwyk; Deirdre Church; Wilson Chan; Dylan R Pillai Journal: Open Forum Infect Dis Date: 2018-10-12 Impact factor: 3.835
Authors: Elisa Serra-Casas; Paulo Manrique; Xavier C Ding; Gabriel Carrasco-Escobar; Freddy Alava; Anthony Gave; Hugo Rodriguez; Juan Contreras-Mancilla; Angel Rosas-Aguirre; Niko Speybroeck; Iveth J González; Anna Rosanas-Urgell; Dionicia Gamboa Journal: PLoS One Date: 2017-10-05 Impact factor: 3.240