PURPOSE: Colorectal cancer (CRC) is one of the major causes of cancer-related deaths in industrialized countries. The identification of small polyps and neoplasms with non-polypoid morphology remains to be a serious challenge, as about a quarter of them are missed during routine colonoscopy. The aim of this study was to evaluate the application of an integrin αvβ3 optical probe in near-infrared fluorescence (NIRF) confocal laser endomicroscopy (CLE) for tumour detection in a rodent model. PROCEDURES: In a novel orthotopic CRC mouse model, tumour growth was monitored by bioluminescence imaging and by colonoscopy employing a rigid white-light endoscope, tumour development was scored by total number and size of tumours. Furthermore, NIRF CLE was established using a fibre probe attached to a confocal laser scanner operating at 660 nm and an antagonistic small-molecule integrin αvβ3 NIRF contrast agent. RESULTS: Three CRC cell lines of different histological origin were successfully implanted in nude mice, proving the power of this new orthotopic model. Whole body NIRF images of tumour-bearing mice showed specific high accumulation of the integrin αvβ3 probe in regions of tumour growth, colocalizing with the bioluminescent signal. Molecular imaging by means of a CLE fibre probe allowed distinguishing normal mucosa structures from tumour tissue, as confirmed by quantitation of fluorescence intensities and histology. CONCLUSIONS: Targeting integrin αvβ3 in a molecular imaging approach was shown to be effective for CRC detection. Use of molecular guidance in near-infrared CLE represents a promising route to improving detection rates.
PURPOSE:Colorectal cancer (CRC) is one of the major causes of cancer-related deaths in industrialized countries. The identification of small polyps and neoplasms with non-polypoid morphology remains to be a serious challenge, as about a quarter of them are missed during routine colonoscopy. The aim of this study was to evaluate the application of an integrin αvβ3 optical probe in near-infrared fluorescence (NIRF) confocal laser endomicroscopy (CLE) for tumour detection in a rodent model. PROCEDURES: In a novel orthotopic CRC mouse model, tumour growth was monitored by bioluminescence imaging and by colonoscopy employing a rigid white-light endoscope, tumour development was scored by total number and size of tumours. Furthermore, NIRF CLE was established using a fibre probe attached to a confocal laser scanner operating at 660 nm and an antagonistic small-molecule integrin αvβ3 NIRF contrast agent. RESULTS: Three CRC cell lines of different histological origin were successfully implanted in nude mice, proving the power of this new orthotopic model. Whole body NIRF images of tumour-bearing mice showed specific high accumulation of the integrin αvβ3 probe in regions of tumour growth, colocalizing with the bioluminescent signal. Molecular imaging by means of a CLE fibre probe allowed distinguishing normal mucosa structures from tumour tissue, as confirmed by quantitation of fluorescence intensities and histology. CONCLUSIONS: Targeting integrin αvβ3 in a molecular imaging approach was shown to be effective for CRC detection. Use of molecular guidance in near-infrared CLE represents a promising route to improving detection rates.
Authors: Wibool Piyawattanametha; Hyejun Ra; Zhen Qiu; Shai Friedland; Jonathan T C Liu; Kevin Loewke; Gordon S Kino; Olav Solgaard; Thomas D Wang; Michael J Mandella; Christopher H Contag Journal: J Biomed Opt Date: 2012-02 Impact factor: 3.170
Authors: Ralf Kiesslich; Martin Goetz; Katharina Lammersdorf; Constantin Schneider; Juergen Burg; Manfred Stolte; Michael Vieth; Bernhard Nafe; Peter R Galle; Markus F Neurath Journal: Gastroenterology Date: 2007-01-31 Impact factor: 22.682
Authors: Martin Goetz; Alex Ziebart; Sebastian Foersch; Michael Vieth; Maximilian J Waldner; Peter Delaney; Peter R Galle; Markus F Neurath; Ralf Kiesslich Journal: Gastroenterology Date: 2009-10-21 Impact factor: 22.682
Authors: Ralf Kiesslich; Juergen Burg; Michael Vieth; Janina Gnaendiger; Meike Enders; Peter Delaney; Adrian Polglase; Wendy McLaren; Daniela Janell; Steven Thomas; Bernhard Nafe; Peter R Galle; Markus F Neurath Journal: Gastroenterology Date: 2004-09 Impact factor: 22.682
Authors: Herlen Alencar; Martin A Funovics; Jose Figueiredo; Heloisa Sawaya; Ralph Weissleder; Umar Mahmood Journal: Radiology Date: 2007-05-16 Impact factor: 11.105
Authors: Kamini Sewda; Domenico Coppola; Steven Enkemann; Binglin Yue; Jongphil Kim; Alexis S Lopez; Jonathan W Wojtkowiak; Valerie E Stark; Brian Morse; David Shibata; Shivakumar Vignesh; David L Morse Journal: Oncotarget Date: 2016-04-05
Authors: Crispin Schneider; Sean P Johnson; Kurinchi Gurusamy; Richard J Cook; Adrien E Desjardins; David J Hawkes; Brian R Davidson; Simon Walker-Samuel Journal: Lasers Surg Med Date: 2016-12-19 Impact factor: 4.025