Duan Wu1, Jianyin Zhou2, Zhenyu Yin, Pingguo Liu, Yilin Zhao, Jianming Liu, Xiaomin Wang. 1. Department of Surgery, Peking 401 Hospital of China Nuclear Industry, Beijing 102413, China. 2. Department of Hepatobiliary Surgery, Affiliated Zhongshan Hospital, Xiamen University, Diagnosis & Treatment of Digestive Diseases Center, Fujian Provincial Key Laboratory of Chronic Liver Disease & Hepatocellular Carcinoma, Xiamen, Fujian 361004, China. Email:zhoujianyin2000@sina.com.
Abstract
OBJECTIVE: To explore the effects and underlying mechanisms of ursodeoxycholic acid on human hepatoma cells. METHODS: HepG2 and SMMC-7721 HCC cell lines were respectively treated with ursodeoxycholic acid. And cell proliferation, apoptosis and the expression of Bax/Bcl-2 gene were detected by methyl thiazolyl tetrazolium (MTT), inverted microscopy, fluorescent microscopy, flow cytometry and Western blot. RESULTS: Ursodeoxycholic acid significantly inhibited the proliferation of human hepatoma cells in a concentration- and time-dependent manner. The half maximal inhibitory concentrations (IC50) of HepG2 and SMMC-7721 were 397.3 and 387.7 µg/ml respectively after a 48-hour treatment of 400 µg /ml ursodeoxycholic acid. And it also induced the apoptosis of HepG2 and SMMC-7721 cells, up-regulated Bax gene and down-regulated Bcl-2 gene. CONCLUSION: Ursodeoxycholic acid inhibits the proliferation of hepatoma cells and induce apoptosis by mitochondrial-mediated pathway.
OBJECTIVE: To explore the effects and underlying mechanisms of ursodeoxycholic acid on humanhepatoma cells. METHODS: HepG2 and SMMC-7721 HCC cell lines were respectively treated with ursodeoxycholic acid. And cell proliferation, apoptosis and the expression of Bax/Bcl-2 gene were detected by methyl thiazolyl tetrazolium (MTT), inverted microscopy, fluorescent microscopy, flow cytometry and Western blot. RESULTS:Ursodeoxycholic acid significantly inhibited the proliferation of humanhepatoma cells in a concentration- and time-dependent manner. The half maximal inhibitory concentrations (IC50) of HepG2 and SMMC-7721 were 397.3 and 387.7 µg/ml respectively after a 48-hour treatment of 400 µg /ml ursodeoxycholic acid. And it also induced the apoptosis of HepG2 and SMMC-7721 cells, up-regulated Bax gene and down-regulated Bcl-2 gene. CONCLUSION:Ursodeoxycholic acid inhibits the proliferation of hepatoma cells and induce apoptosis by mitochondrial-mediated pathway.