Michelle Cardoso de Sousa1, Rafael Brizola Vieira2, Danielle Sá Dos Santos3, Claudio Antonio Talge Carvalho4, Samira Esteves Afonso Camargo5, Maria Nadir Gasparoto Mancini6, Luciane Dias de Oliveira7. 1. Department of Biosciences and Oral Diagnosis, School of Dentistry of São José dos Campos, UNESP - Univ Estadual Paulista, Engenheiro Francisco José Longo 777, São Dimas, São José dos Campos, Cep 12245-000 SP, Brazil. Electronic address: mikafosjc@yahoo.com.br. 2. Department of Biosciences and Oral Diagnosis, School of Dentistry of São José dos Campos, UNESP - Univ Estadual Paulista, Engenheiro Francisco José Longo 777, São Dimas, São José dos Campos, Cep 12245-000 SP, Brazil. Electronic address: fael_briza@hotmail.com. 3. Department of Biosciences and Oral Diagnosis, School of Dentistry of São José dos Campos, UNESP - Univ Estadual Paulista, Engenheiro Francisco José Longo 777, São Dimas, São José dos Campos, Cep 12245-000 SP, Brazil. Electronic address: danni.sa@bol.com.br. 4. Department of Restorative Dentistry, School of Dentistry of São José dos Campos, UNESP - Univ Estadual Paulista, Engenheiro Francisco José Longo 777, São Dimas, São José dos Campos, Cep 12245-000 SP, Brazil. Electronic address: claudiotalge@fosjc.unesp.br. 5. Department of Biosciences and Oral Diagnosis, School of Dentistry of São José dos Campos, UNESP - Univ Estadual Paulista, Engenheiro Francisco José Longo 777, São Dimas, São José dos Campos, Cep 12245-000 SP, Brazil. Electronic address: samira@fosjc.unesp.br. 6. Department of Biosciences and Oral Diagnosis, School of Dentistry of São José dos Campos, UNESP - Univ Estadual Paulista, Engenheiro Francisco José Longo 777, São Dimas, São José dos Campos, Cep 12245-000 SP, Brazil. 7. Department of Biosciences and Oral Diagnosis, School of Dentistry of São José dos Campos, UNESP - Univ Estadual Paulista, Engenheiro Francisco José Longo 777, São Dimas, São José dos Campos, Cep 12245-000 SP, Brazil. Electronic address: luciane@fosjc.unesp.br.
Abstract
BACKGROUND: The aim of this study was to investigate enzymatic and non-enzymatic antioxidant systems and levels of biomarker levels of oxidative damage in the saliva of patients with Down's syndrome (DS). METHODS: Saliva samples were collected from 30 patients with DS and control group (age: 14-24 years). Subsequently, the concentrations of superoxide dismutase, concentration of malondialdehyde, carbonylated proteins, uric acid, vitamin C and total protein, peroxidase activity and total antioxidant capacity were analyzed. RESULTS: Patients with DS presented significantly higher concentrations of superoxide dismutase, higher levels of malondialdehyde and salivary total protein content than controls (p<0.05). Conversely, no difference in carbonylated proteins or antioxidants (uric acid, vitamin C, peroxidase, and total antioxidant capacity) was observed between DS patients and controls (p>0.05). CONCLUSION: Patients with DS are more vulnerable to oxidative stress in saliva as indicated by the significant increase in malondialdehyde and superoxide dismutase concentrations found in this study.
BACKGROUND: The aim of this study was to investigate enzymatic and non-enzymatic antioxidant systems and levels of biomarker levels of oxidative damage in the saliva of patients with Down's syndrome (DS). METHODS: Saliva samples were collected from 30 patients with DS and control group (age: 14-24 years). Subsequently, the concentrations of superoxide dismutase, concentration of malondialdehyde, carbonylated proteins, uric acid, vitamin C and total protein, peroxidase activity and total antioxidant capacity were analyzed. RESULTS:Patients with DS presented significantly higher concentrations of superoxide dismutase, higher levels of malondialdehyde and salivary total protein content than controls (p<0.05). Conversely, no difference in carbonylated proteins or antioxidants (uric acid, vitamin C, peroxidase, and total antioxidant capacity) was observed between DS patients and controls (p>0.05). CONCLUSION:Patients with DS are more vulnerable to oxidative stress in saliva as indicated by the significant increase in malondialdehyde and superoxide dismutase concentrations found in this study.