Weiyue Wu1, Benxiang Hou2. 1. Department of Endodontics, Capital Medical University School of Stomatology, Beijing 100050, China. 2. Email: houbenxiang@gmail.com.
Abstract
OBJECTIVE: To investigate the microbial composition and differences in dental plaque of caries and caries-free adults. METHODS: Caries-active (n = 16) was defined as an individual who had at least three untreated decayed tooth and decayed-missing-filled-tooth (DMFT) score greater than 6. Caries-free (n = 16) was defined as an individual who had a DMFT score equal to zero. The patients were 18-35 years old. Samples from supra-gingival plaque were obtained and PCR-denaturing gel electrophoresis cloning and sequencing of caries pathogens were used to catch the core microbial of dental caries. RESULTS: Six phylum, 28 genus and 88 species were detected. In caries group, Prevotella, Capnocytophaga, Actinomyces, Veillonella and Corynebacterium were predominant, accounting for 56.2% (334/594) of the total cloning number of caries group. Caries-free group contained more predonminant genus than caries group. Prevotella, Veillonella, Capnocytophaga, Corynebacterium, Streptococcus, Actinomyces, Aggregatibacter and Neisseria were predominant, accounting for 65.2% (354/543) of the total cloning number of caries-free group. Caries group had less diversity than the caries-free group. The difference was statistically significant (P < 0.05). CONCLUSIONS: Caries might be caused by potentially pathogenic microbial communities rather than a single pathogen. In the progress of dental caries, the microbial diversity decreased.
OBJECTIVE: To investigate the microbial composition and differences in dental plaque of caries and caries-free adults. METHODS: Caries-active (n = 16) was defined as an individual who had at least three untreated decayed tooth and decayed-missing-filled-tooth (DMFT) score greater than 6. Caries-free (n = 16) was defined as an individual who had a DMFT score equal to zero. The patients were 18-35 years old. Samples from supra-gingival plaque were obtained and PCR-denaturing gel electrophoresis cloning and sequencing of caries pathogens were used to catch the core microbial of dental caries. RESULTS: Six phylum, 28 genus and 88 species were detected. In caries group, Prevotella, Capnocytophaga, Actinomyces, Veillonella and Corynebacterium were predominant, accounting for 56.2% (334/594) of the total cloning number of caries group. Caries-free group contained more predonminant genus than caries group. Prevotella, Veillonella, Capnocytophaga, Corynebacterium, Streptococcus, Actinomyces, Aggregatibacter and Neisseria were predominant, accounting for 65.2% (354/543) of the total cloning number of caries-free group. Caries group had less diversity than the caries-free group. The difference was statistically significant (P < 0.05). CONCLUSIONS: Caries might be caused by potentially pathogenic microbial communities rather than a single pathogen. In the progress of dental caries, the microbial diversity decreased.