Association between low C-peptide and low lumbar bone mineral density in postmenopausal women without diabetes.

UNLABELLED: In this population-based, cross-sectional study in Italian postmenopausal females not affected by diabetes, we showed a link between serum C-peptide and lumbar bone mineral density, suggesting that C-peptide exerts an insulin-independent effect on bone mass.
INTRODUCTION: It is well known that type 1 (T1) diabetes, characterized by insulin and C-peptide deficiency, is associated with a low lumbar bone mineral density and an increased risk for fracture. While a role for insulin in the pathogenesis of osteoporosis has been demonstrated, the association between C-peptide and the bone mineral density has not been investigated. We conducted a study in a cohort of 84 postmenopausal women without diabetes to clarify the association between serum C-peptide and the lumbar bone mineral density.
METHODS: Participants underwent a bone mineral density evaluation by DXA and biochemical analysis including the C-peptide assay.
RESULTS: rteen percent of the population had osteoporosis and 38% had osteopenia. With ANOVA test, we showed that women with the lowest C-peptide concentration had lower lumbar mineral density in comparison to those in all other C-peptide concentration group (p = 0.02 among groups after adjustment). The univariate and multivariate analysis showed that C-peptide was positively associated with both lumbar T-score and Z-score besides other well-known factors like age (with T-score p < 0.001; beta = -0.38) and BMI (with T-score p = 0.009; beta = 0.34), while insulin was not correlated with the lumbar bone mineral density. The area under the receiver operating characteristic (ROC) curve for C-peptide to predict the absence of lumbar osteoporosis was 0.74 (SE = 0.073; p = 0.013).
CONCLUSIONS: These results suggest that C-peptide may exert an insulin- and BMI-independent effect on lumbar bone mineral density and that further large-scale studies are needed in order to clarify its role in bone mineralization especially in subjects without diabetes.

Introduction

Several studies have shown opposite results for type 1 (T1D) and type 2 diabetes (T2D) concerning the appearance of osteoporosis. In fact, it has been shown that a declined bone mineral density (BMD) associated with an increase in fracture risk in T1D [1-3] while an unpredictable BMD change and a variable fracture risk have been reported in T2D [2, 4].

In particular, it has been shown that the lumbar bone mineral density (LBMD) is lower in adolescents and adults with T1D compared to control population [5-7].

Insulin is crucial in osteoblast differentiation from marrow stromal cells [8]; thus, its role in the pathogenesis of osteoporosis in diabetes has been proposed [9]. However, insulin and C-peptide are secreted in equimolar concentrations from the pancreas. Since C-peptide has irrelevant extraction by the liver, constant peripheral clearance and its half-life is longer than insulin [10-12], it is commonly used in preference to insulin assessment. In addition, C-peptide has a central function in the synthesis and correct folding of insulin; therefore, a role also for C-peptide may be hypothesized in osteoporosis. Recent studies have demonstrated specific binding of C-peptide to cell surfaces(to pancreatic islets and red blood cells of rat and to cultured human renal tubular cells, fibroblasts, and endothelial cells) with the subsequent stimulation of specific intracellular processes involving Na+-K+-ATPase activity, a key mechanism for bone mineralization [13, 14]. This finding may explain the positive correlation between both LBMD and femoral bone mineral density (FBMD) with C-peptide in patients with T1D [15]. However, a recent investigation has shown that serum C-peptide was negatively associated with BMD in individuals without diabetes and that this association was independent of the serum insulin levels and dependent of age [16]. Thus, at this moment, the definitive link between C-peptide and BMD is not yet clarified and needs to be investigated. Consequently, we conducted a study in a cohort of postmenopausal women without diabetes to clarify the eventual association between serum C-peptide and both LBMD and FBMD. Furthermore, we examined whether other factors such parathyroid hormone (PTH), 25-OH vitamin D (the most important regulators of calcium homeostasis), and the fibroblast growth factor 23 (FGF-23) (the main phosphate-regulating hormone) [17] were affecting this association.

Methods

This survey is in progress since 2011 in the regions of Lombardia and Calabria, Italy, and involves white postmenopausal female volunteers aged more than 45 years, invited by newspapers ads to participate in the study and consecutively enrolled. The subjects of this investigation consisted of the first 84 postmenopausal women without diabetes, who completed all the examinations at May 2014.

All participants underwent a brief interview to provide information about familiarity for osteoporosis, current physical exercise activity as the usual daily time spent outside walking (more than 30 min) [18], smoke habits, medications use, and history of fractures. Pathological or high-energy fractures and fractures in sites not commonly associated with osteoporosis were not considered in the statistical analysis. Postmenopausal status was defined as the presence of a serum follicle-stimulating hormone (FSH) level of over 40 IU/l (if available) or no natural menses for at least 1 year.

Subjects were excluded if they had self-report diagnosis of T1D or T2D and/or they were taking antidiabetic medication and/or insulin or they had fasting plasma glucose concentration equal or more than 126 mg/dl, or any clinical condition that affects bone metabolism, such as diseases of the kidney, liver, thyroid, or parathyroids, rheumatic diseases, malabsorption syndromes, malignant tumors, and hematological diseases. None were taking drugs or hormones that influence bone metabolism, such as glucocorticoids, estrogens, thyroid hormone, fluoride, bisphosphonate, calcitonin, thiazide diuretics, barbiturates, vitamin D, or calcium-containing drugs. All participants underwent both the LBMD and FBMD assessment, and a blood sample was collected for the biochemical analysis. We obtained their informed consent to participate in the study. The study was approved by both the Ethic Committee ASL Milan 2 and the University Hospital Mater Domini, Catanzaro. The investigation conforms to the principles outlined in the Declaration of Helsinki.

DXA assessment

Postmenopausal women underwent a BMD evaluation of the lumbar spine and left femur by DXA (Hologic QDR Inc., MA, USA, 17). BMD was expressed as the amount of mineral (g) divided by the area scanned (cm2). Bone density was then expressed as the T-score, calculated on the basis of the normal reference values. The T-score is defined as the number of standard deviations from the healthy young adult mean (normal, > −1; osteopenia, −1 to −2.49; osteoporosis, ≤ −2.5) while the Z-score was the number of standard deviations in comparison to healthy women of the same age [19, 20]. The instrument was calibrated every day in accordance with the manufacturer’s recommendations. The in vivo precision was established on the basis of repeated measurements in 30 women and was <1 %.

Nutritional calcium intake and anthropometric measurements

The participant’s nutritional calcium intake was calculated using the nutritional software MetaDieta 3.0.1 (MeTeDa srl, S. Benedetto del Tronto, Italy) [21] and categorized into two groups (normal intake-low intake). Body weight was measured before breakfast with the subjects lightly dressed, subtracting the weight of clothes. Body weight was measured with a calibrated scale and height measured with a wall-mounted stadiometer. BMI was calculated with the following equation: weight (kg) / (height (m))2

Biochemical evaluation

Venous blood was collected after fasting overnight into Vacutainer tubes (Becton & Dickinson) and centrifuged within 2 h. Serum glucose, phosphate, and hemoglobin A1c (HbA1c) were measured by standard laboratory techniques (enzymatic colorimetric test) [22]. Serum calcium (reference values (r.v.) 8.9–10.1 mg/dL) was evaluated with a colorimetric assay according to Schwarzenbach, using o-cresolphthalein-complexone [23]. Serum insulin was assessed with cheminumilescent microparticle immunoassay (CMIA) (ARCHITECT insulin assay Abbott Laboratories, assay precision of ≤7 % total coefficient variation (CV)). Standard C-peptide assay was performed with CMIA (ARCHITECT C-peptide assay Abbott, Germany; assay precision of ≤10 % total CV). Concentrations of soluble FGF-23 (r.v. 10–50 pg/ml) and N-terminal telopeptide (NTx) (r.v. 19–63 nM BCE/nM creatinine) in serum were determined by commercial ELISA assay (Human FGF-23 (C-term) ELISA Kit, San Clemente, CA, USA; Osteomark NTx serum ELISA, Wampole Laboratories, Princeton, NJ, USA). The intact PTH (r.v. 15–65 pg/mL) and 25-hydroxyvitamin D [(25OH)D] (r.v. 30.0–100.0 ng/mL) were measured by radioimmunoassay [24]. Internal quality control (IQC) was performed every day for all determination.

Statistical analysis

Data are reported as mean ± standard deviation (SD). Eighty-five subjects are required to detect a correlation between C-peptide and LBMD and FBMD equal to 0.30, considered to be clinically meaningful, with 80 % power on a two-sided level of significance of 0.05.

A chi-square test was performed to analyze the difference of the prevalence between groups. The ANOVA was used to compare the means between groups (on the basis of C-peptide concentration tertiles) with Sheffe’s post hoc test. Pearson correlation was used to identify the variables correlated to both the LBMD and FBMD expressed as T-score and Z-score (the number of SDs) given that the continuous variables were normally distributed. Non-normally distributed data were log transformed. The multivariate linear stepwise regression analysis was used to test the association between C-peptide and BMD adjusting for confounding variables selected from univariate analysis having a p < 0.1 (in model I), considering LBMD and FBMD (as T-score or Z-score) as dependent variables, in the separate analysis. In model II, in the multivariate linear stepwise regression analysis, we included also the log-transformed insulin. In model III, in the multivariate linear stepwise regression analysis we included, among confounding variables, also smoking, physical exercise, and familiarity for osteoporosis. The general linear model (GLM) was used to adjust LBMD (T-score value) for BMI and glucose. Furthermore, the area under the receiver operating characteristic (ROC) curve was used to analyze the capacity of C-peptide to predict the absence of osteoporosis at lumbar spine. Significant differences were assumed to be present at p < 0.05 (two-tailed). All comparisons were performed using SPSS 20.0 for Windows (S. Wacker Drive, Chicago, IL 60606, USA).

Results

The mean age of the population was 56 ± 6 years. A total of 12 subjects (14 %) had osteoporosis, 32 (38 %) had osteopenia, and 40 (48 %) had a normal BMD. Hypovitaminosis D was present in 18, 40, and 42 % and familiarity for osteoporosis in 7, 13, and 50 % of normal, osteopenic, and osteoporotic subjects, respectively. Only one subject did not complete all the examinations. Table 1 shows the characteristics of the study population. At univariate (Table 2, showing only factors significantly correlated to BMD) and multivariate (Table 3) analysis, after adjusting for confounding factors, there was no association between C-peptide and FBMD (both T-score, see Table 3, and Z-score, not showed), while C-peptide was associated with LBMD (with both T-score and Z-score, see Table 3) as well as age and BMI (with T-score: C-peptide p = 0.01, beta = 0.33; age p < 0.001, beta = −0.38; BMI p = 0.009, beta = 0.34; variance inflation factor—VIF = 1.91, 1.00, 1.92 for C-peptide, age, and BMI, respectively; tolerance = 0.52, 0.99, 0.52 for C-peptide, age, and BMI, respectively. Model I, Table 3). Interestingly, log-transformed insulin was not correlated with LBMD (with T-score: p = 0.66; beta 0.072; t = 0.43, ,model II). In the multivariate analysis, the correlation between LBMD (measured by T-score) and glucose disappeared (models I, II, and III of Table 3, see legend). In addition, PTH, FGF-23, and 25-OH vitamin D were not correlated (Table 2). Since BMI result correlated to C-peptide (r = 0.63, p < 0.001), we performed a multivariate analysis with C-peptide corrected for BMI and the correlation did not change. To confirm all these associations, we categorized the population according to C-peptide tertiles. In Table 4, the prevalence of the risk factors for osteoporosis according to C-peptide tertiles is showed. Consequently, in the multivariate analysis, we further adjusted the data for smoke, physical exercise, and familiarity (Table 4) but these factors did not affect the associations (model III in Table 3, see legend, data not showed).

Table 1

Characteristics of whole non-diabetic population (N = 84)

Variables	Mean	SD
Age (years)	53	6
Weight (Kg)	66.0	14
Height (cm)	161	7
BMI (kg/m2)	25	5
Lumbar T-score	−0.94	1
Lumbar Z-score	0.03	1
Femur Neck T-score	−1.13	1
Femur neck Z-score	−0.25	1
Phosphorus (mg/dL)	3.6	0.4
Calcium (mg/dL)	9.4	0,4
Glucose (mg/dL)	91.2	9
Insulin (μU/mL)	8.4	4
C-peptide (ng/mL)	1.82	0.7
HbA1c (%)	5.6	0.2
PTH (pg/mL)	45.27	19
Vitamin D (ng/mL)	26.4	10
NTx (nM BCE/nM creatinine)	27.6	7
FGF-23 (pg/mL)	9.2	6

Table 2

Univariate analysis—Pearson correlation

Variables		Age	BMI	Insulin	C-peptide	Calcium	Vitamin D	Glucose
LBMD (Z-score)	r	–	0.53	0.50	0.51	–	–	0.25
	p	–	<0.001	<0.001	<0.001	–	–	0.01
LBMD (T-score)	r	−0.3	0.48	0.47	0.48	−0.19	–	0.20
	p	0.001	<0−001	<0.001	<0.001	0.07	–	0.05
FBMD (T-score)	r	−0.37	0.47	0.38	0.34	−0.19	−0.23	–
	p	<0.001	<0.001	0.001	0.001	0.07	0.03	–

Table 3

Multivariate linear regression analysis—factors correlated to both LBMD and FBMD

Dependent variables	Independent variables	B value	β value	t value	p value
LBMD T-scorea	Age	−0.08	−0.38	−4.2	<0.001
	BMI	0.08	0.34	2.6	0.009
	C-peptide	0.60	0.33	2.5	0.01
LBMD Z-scoreb	BMI	0.08	0.34	2.8	0.005
	C-peptide	0.50	0.29	2.4	0.01
FBMD T-scorec	Age	−0.06	−0.39	−4.5	<0.001
	BMI	0.10	0.48	5.5	<0.001

aExcluded variables—model I: calcium, glucose; model II: calcium, log insulin, glucose; model III: calcium, log insulin, glucose, smoke, physical exercise, familiarity

bExcluded variables—model I: log insulin, glucose; model II: log insulin, glucose, smoke, physical exercise, familiarity

cExcluded variables—model I: calcium, log insulin, vitamin D, C-peptide; model II: calcium, log insulin, vitamin D, C-peptide, smoke, physical exercise, familiarity

Table 4

Risk factor prevalence according to C-peptide tertiles

Risk factors	I tertile N = 28	II tertile N = 28	III tertile N = 28	p value
Current smokers (%)	32	21	7	0.03 vs no smokers
Normal calcium intake (%)	67	60	71	0.33 vs low calcium intake
Exercise habitually(%)	53	46	28	0.02 vs no exercise
Fractures (%)	0	3	0	0.33 vs no fractures
Familiar history for osteoporosis (%)	17	10	0	0.05 vs no familiar history

Table 5 shows the characteristic of the population according to C-peptide concentration tertiles. Analysis by ANOVA revealed that women with the lowest C-peptide concentration (tertile I) had lower average LBMD (both T-score and Z-score) in comparison to those in all other tertiles (p = 0.03 among tertiles; tertile I vs tertile III p < 0.001; tertile II vs tertile III p = 0.019, respectively; Table 5 and Fig. 1). Because of the significant difference in the BMI and glucose among C-peptide tertiles (Table 5), a GLM was performed to adjust LBMD (T-score value) for these factors, confirming the lower LBMD in those with the lowest C-peptide (p = 0.02 after adjustment, Table 5). The area under the ROC curve for C-peptide to predict the absence of lumbar osteoporosis was 0.74 (SE = 0.073; p = 0.013). The C-peptide equal to 1.31 achieved satisfactory sensitivity (75 %) and specificity (60 %) (Fig. 2).

Table 5

Characteristics of the whole population according to C-peptide concentration tertiles—ANOVA

Variables	I tertile	II tertile	III tertile	p value
C-peptide range (ng/ml)	0.80–1.36	1.40–2.02	2.03–3.80
Age (years)	54 ± 7	53 ± 5	52 ± 5	0.69
BMI (kg/m2)	25.7 ± 5	22.9 ± 3	26.4 ± 5	0.02
LBMD T-score	−1.28 ± 1	−1.10 ± 1	−0.43 ± 1	0.03 0.02a
LBMD Z-score	−0.25 ± 1	−0.14 ± 1	0.51 ± 1	0.04
FBMD T-score	−1.15 ± 1	−1.30 ± 1	−0.95 ± 1	0.50
FBMD Z-score	−0.27 ± 0.9	−0.42 ± 1	−0.06 ± 1	0.43
Calcium (mg/dL)	9.4 ± 0.3	9.4 ± 0.4	9.3 ± 0.4	0.78
Phosphorus (mg/dL)	3.6 ± 0.4	3.5 ± 0.5	3.7 ± 0.3	0.35
Glucose (mg/dL)	88.2 ± 8	89.1 ± 9	96.3 ± 9	0.003
Insulin (μU/mL)	6.5 ± 3	7.1 ± 2	11.9 ± 4	<0.001
C-peptide (ng/mL)	1.3 ± 0.4	1.6 ± 0.3	2.5 ± 0.5	<0.001
HbA1c (%)	5.6 ± 0.2	5.6 ± 0.3	5.7 ± 0.3	0.69
PTH (pg/mL)	47.6 ± 18	45.8 ± 21	42.1 ± 18	0.54
25OH vit D (ng/mL)	25.1 ± 11	25.9 ± 9	27.9 ± 11	0.59
NTx (nM BCE/nM creatinine)	25.8 ± 8	27.5 ± 6	28.9 ± 7	0.37
FGF-23 (pg/mL)	10.6 ± 8	8.1 ± 4	9.3 ± 5	0.35

aLBMD: T-score, glucose, and BMI adjusted in the GLM

Fig. 1

Scatter plot with the individual data of the correlation between LBMD and C-peptide

Fig. 2

The area under the ROC curve for C-peptide to predict the lumbar osteoporosis

Discussion

The results of this population-based, cross-sectional study in Italian postmenopausal females not affected by diabetes showed that serum C-peptide was strongly associated with LBMD (with both T-score and Z-score value) (Table 2) also after adjustment for several factors (Table 3). In addition, the main calcium-phosphate regulating hormones (PTH, 25-OH vitamin D, and the FGF-23) did not affect this association that was also independent of the serum insulin. We also found that postmenopausal women in the lowest serum C-peptide concentration tertile had the lower LBMD in comparison to those in the highest C-peptide tertiles (Table 5). With these results, we infer that a low C-peptide concentration could be a novel osteopenic marker in postmenopausal women not affected by diabetes, if confirmed.

It is well known that T1D, that is characterized by an insulin and C-peptide deficiency, is associated with a low LBMD and an increased risk for fracture [1, 2, 5–7]. A role for insulin in osteoblastogenesis has been proposed [25]. Furthermore, recent investigations have suggested that C-peptide is not biologically inert but that it is a separate entity with different characteristics from those of insulin [13]. It has been shown that C-peptide involves the activation of Ca2+-dependent signaling pathways with consequent stimulation of Na+-K+-ATPase [13] via specific binding as a ligand to its receptor. The transfer of inorganic phosphate into osteoblastic cells, important in bone formation, is related to the transmembrane electrochemical gradient of sodium maintained by Na,K-ATPase [14]. In addition, bone mineralization critically depends upon optimal calcium homeostasis in osteoblasts. To establish optimal intracellular [Ca2+], osteoblasts need Ca-ATPase and Na/Ca exchange systems whose activities depend on the activity of Na,K-ATPase [26]. Thus, C-peptide may be not only a marker but it may also have an active role in the development of osteoporosis. However, whether C-peptide acts also in concert with insulin remains to be elucidated. In this contest, it is important reflect also on the role of the islet amyloid pancreatic polypeptide (IAPP, amylin) that is a hormone co-secreted with both insulin and C-peptide from beta cells. Patients with T1D are also deficient in IAPP. Amylin has been implicated in bone physiology. In fact, genetic ablation of amylin leads to bone loss in mice [27] and treatment with amylin can in part reverse bone loss in ovariectomized rats [28]. Analysis of amylin gene supports an evolutionary relationship to calcitonin [29]. In addition, a positive association between amylin levels and BMD in women with anorexia nervosa has been reported [30]. Thus, a number of investigations suggest a role for the pancreatic hormones on bone metabolism.

A potential explanation of link between C-peptide and LBMD may be the role of BMI conditioning the association. In fact, hyperinsulinemia and insulin resistance are highly correlated with BMI [31] and, in turn, they affect the bone. As expected, in our investigation, we found a strong correlation between BMI and C-peptide. To exclude any interference, we corrected C-peptide for BMI but the results did not change. Therefore, although BMI may be a confounding factor, there is sufficient evidence to suggest that C-peptide may exert a BMI-independent effect on bone mass, providing a further link between nutrient intake and bone turnover. In this regard, it has been showed that C-peptide predicted visceral fat accumulation in both short-term and long-term follow-up [32]; thus, the hormonal changes may precede and predict the BMI increase as suggested from other studies showing that the secretion of bone-active hormones from the pancreas may explain part of the relationship between fat mass and bone mass [31]. However, further large-scale studies need to be performed in order to clarify the role of the hormones secreted by beta cells on bone metabolism especially in subjects not affect by diabetes.

Of course, the link between C-peptide and BMD is still neglected and needs to be studied in deep in experimental study. In a recent investigation, using data from the NHANES [16], a negative association between serum C-peptide and BMD (expressed in g/cm2) was found independent of the serum insulin levels. However, in the 40–59 years age group, the group similar to our population, the authors showed that, after additional data adjustment, the serum C-peptide levels were not associated with the spinal BMD [16]. In that study, there was a lack of information about the measurement of serum C-peptide [16]. Our study differs from that investigation in several points: We studied specifically postmenopausal women without diabetes and in a definite age range; we reported plasma glucose concentration of the whole population (that was in normal range); we defined low BMD according to the T-score and Z-score; whole-body DXA scan was not performed in our study; we assessed C-peptide with a definite methods. Finally, we investigated the effects of the interaction with other factors on BMD like PTH, 25-OHvitamin D, and FGF-23. Our findings are in agreement with a previous investigation [15] showing a positive correlation between both LBMD and FBMD and C-peptide levels in patients with T1D. C-peptide, insulin, and amylin seem to be associated with BMD in the same manner. C-peptide is commonly used in clinical practice in preference to insulin assessment [10-12]. Thus, in the future, C-peptide may be also measured to predict lumbar osteoporosis, if its role will be confirmed. In our population, we found a positive correlation between C-peptide and NTx (r = 0.22 and p = 0.05, data not showed) while a negative correlation was expected. However, this finding is plausible since we showed that postmenopausal women of the I tertile (those with the lower C-peptide concentration and in which a greater NTx was expected) exercise habitually more than those of all other groups (Table 3). In this regard, several studies reported that both the walking exercise and a high-impact exercise training decreased NTx levels in postmenopausal osteopenic women [33, 34]. Physical exercise appears to suppress bone turnover; thus, it is plausible that in our investigation, women in the I tertile had also lower NTx concentration (Table 2; I vs III tertile, p = 0.009, in the post hoc analysis), affecting the correlation between C-peptide and NTx.

This study has several limitations that must be addressed. The study could not determine causality. Furthermore, our study was limited by its small size and cross-sectional design. However, the statistical analysis is robust and adequate. The clinical significance of the low C-peptide in osteoporosis could be well deduced from the present results. The investigation was done on representative samples of the Italian population; in fact, the study was conducted across two institutions, potentially increasing the generalizability of our findings from a geographical perspective. Our results were not purely random as established by previous investigations [13, 26] and further confirmed by multiple analyses (as showed by the statistical power and by the absence of collinearity and interaction between variables, see the “Results” section). Of course, this study may serve to generate hypotheses and documenting this observation in “in vitro” research in the future may be of great interest.

Unfortunately, we did not assess any marker of bone formation but one of resorption alone as NTx. However, this is in line with other investigations [35, 36].

Further studies are warranted to investigate the potential relationship between C-peptide and bone loss in other population and to confirm that C-peptide could become a helpful clinical tool in the assessment of osteoporosis.