Nakarin Suwannarach1,2, Boonsom Bussaban1,2,3, Wipornpan Nuangmek4, Wasna Pithakpol4, Bantoon Jirawattanakul5, Kenji Matsui6, Saisamorn Lumyong1,2,3. 1. Department of Biology, Faculty of Science, Chiang Mai University, Chiang Mai 50200, Thailand. 2. Postharvest Technology Innovation Center, Commission on Higher Education, Bangkok 10400, Thailand. 3. Postharvest Technology Research Institute, Chiang Mai University, Chiang Mai 50200, Thailand. 4. Faculty of Agriculture and Natural Resources, University of Phayao, Phayao 56000, Thailand. 5. Chiang Mai Thanatorn Co., Ltd, 98 M.17 Mae Soon, Fang, Chiang Mai 50110, Thailand. 6. Graduate School of Medicine and Department of Biological Chemistry, Faculty of Agriculture, Yamaguchi University, Yamaguchi 753-8515, Japan.
Abstract
BACKGROUND: This study investigated both the in vitro and in vivo biofumigant ability of the endophytic fungus Muscodor suthepensis CMU-Cib462 to control Penicillium digitatum, the main cause of tangerine fruit rot. RESULTS: Volatile compounds from M. suthepensis inhibited mycelial growth of the pathogen. The most abundant compound was 2-methylpropanoic acid, followed by 3-methylbutan-1-ol. They showed median effective doses (ED50) on P. digitatum growth of 74.91 ± 0.73 and 250.29 ± 0.29 µL L(-1) airspace respectively. Rye grain was found to be a suitable solid medium for M. suthepensis inoculum production. The results indicated that mycofumigation with a 30 g rye grain culture of M. suthepensis for 12 h controlled tangerine fruit rot. The percentage weight loss and soluble solids concentration of fumigated tangerines were similar to those of non-infected and non-fumigated fruits. CONCLUSION: Muscodor suthepensis has potential as a biofumigant for controlling postharvest disease of tangerine fruit.
BACKGROUND: This study investigated both the in vitro and in vivo biofumigant ability of the endophytic fungus Muscodor suthepensis CMU-Cib462 to control Penicillium digitatum, the main cause of tangerine fruit rot. RESULTS: Volatile compounds from M. suthepensis inhibited mycelial growth of the pathogen. The most abundant compound was 2-methylpropanoic acid, followed by 3-methylbutan-1-ol. They showed median effective doses (ED50) on P. digitatum growth of 74.91 ± 0.73 and 250.29 ± 0.29 µL L(-1) airspace respectively. Rye grain was found to be a suitable solid medium for M. suthepensis inoculum production. The results indicated that mycofumigation with a 30 g rye grain culture of M. suthepensis for 12 h controlled tangerine fruit rot. The percentage weight loss and soluble solids concentration of fumigated tangerines were similar to those of non-infected and non-fumigated fruits. CONCLUSION:Muscodor suthepensis has potential as a biofumigant for controlling postharvest disease of tangerine fruit.
Authors: Aleksandra Steglińska; Katarzyna Pielech-Przybylska; Regina Janas; Mieczysław Grzesik; Sebastian Borowski; Dorota Kręgiel; Beata Gutarowska Journal: Molecules Date: 2022-06-09 Impact factor: 4.927