Literature DB >> 25616078

Enzymatic cleavage of uracil-containing single-stranded DNA linkers for the efficient release of affinity-selected circulating tumor cells.

Soumya V Nair1, Małgorzata A Witek, Joshua M Jackson, Maria A M Lindell, Sally A Hunsucker, Travis Sapp, Caroline E Perry, Mateusz L Hupert, Victoria Bae-Jump, Paola A Gehrig, Weiya Z Wysham, Paul M Armistead, Peter Voorhees, Steven A Soper.   

Abstract

We report a novel strategy to enzymatically release affinity-selected cells, such as circulating tumor cells (CTCs), from surfaces with high efficiency (∼90%) while maintaining cell viability (>85%). The strategy utilizes single-stranded DNAs that link a capture antibody to the surfaces of a CTC selection device. The DNA linkers contain a uracil residue that can be cleaved.

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Year:  2015        PMID： 25616078      PMCID： PMC4552044          DOI： 10.1039/c4cc09765c

Source DB:  PubMed          Journal:  Chem Commun (Camb)        ISSN： 1359-7345            Impact factor:   6.222


  27 in total

1.  Fabrication of DNA microarrays onto poly(methyl methacrylate) with ultraviolet patterning and microfluidics for the detection of low-abundant point mutations.

Authors:  Catherine Situma; Yun Wang; Mateusz Hupert; Francis Barany; Robin L McCarley; Steven A Soper
Journal:  Anal Biochem       Date:  2005-05-01       Impact factor: 3.365

Review 2.  Merging microfluidics with microarray-based bioassays.

Authors:  Catherine Situma; Masahiko Hashimoto; Steven A Soper
Journal:  Biomol Eng       Date:  2006-07-07

3.  Controlled capture and release of cardiac fibroblasts using peptide-functionalized alginate gels in microfluidic channels.

Authors:  Brian D Plouffe; Melissa A Brown; Rohin K Iyer; Milica Radisic; Shashi K Murthy
Journal:  Lab Chip       Date:  2009-03-12       Impact factor: 6.799

4.  Detachment of captured cancer cells under flow acceleration in a bio-functionalized microchannel.

Authors:  Luthur Siu Lun Cheung; Xiangjun Zheng; Ashley Stopa; James C Baygents; Roberto Guzman; Joyce A Schroeder; Ronald L Heimark; Yitshak Zohar
Journal:  Lab Chip       Date:  2009-03-18       Impact factor: 6.799

Review 5.  The mechanisms of UV mutagenesis.

Authors:  Hironobu Ikehata; Tetsuya Ono
Journal:  J Radiat Res       Date:  2011       Impact factor: 2.724

6.  High-throughput selection, enumeration, electrokinetic manipulation, and molecular profiling of low-abundance circulating tumor cells using a microfluidic system.

Authors:  Udara Dharmasiri; Samuel K Njoroge; Małgorzata A Witek; Morayo G Adebiyi; Joyce W Kamande; Mateusz L Hupert; Francis Barany; Steven A Soper
Journal:  Anal Chem       Date:  2011-02-14       Impact factor: 6.986

Review 7.  Fabrication of DNA microarrays onto polymer substrates using UV modification protocols with integration into microfluidic platforms for the sensing of low-abundant DNA point mutations.

Authors:  Steven A Soper; Masahiko Hashimoto; Catherine Situma; Michael C Murphy; Robin L McCarley; Yu-Wei Cheng; Francis Barany
Journal:  Methods       Date:  2005-09-29       Impact factor: 3.608

8.  Immobilized molecular beacons: a new strategy using UV-activated poly(methyl methacrylate) surfaces to provide large fluorescence sensitivities for reporting on molecular association events.

Authors:  Catherine Situma; Amanda J Moehring; Mohamed A F Noor; Steven A Soper
Journal:  Anal Biochem       Date:  2006-12-20       Impact factor: 3.365

9.  Isolation and characterization of endonuclease VIII from Escherichia coli.

Authors:  R J Melamede; Z Hatahet; Y W Kow; H Ide; S S Wallace
Journal:  Biochemistry       Date:  1994-02-08       Impact factor: 3.162

10.  Highly efficient circulating tumor cell isolation from whole blood and label-free enumeration using polymer-based microfluidics with an integrated conductivity sensor.

Authors:  André A Adams; Paul I Okagbare; Juan Feng; Matuesz L Hupert; Don Patterson; Jost Göttert; Robin L McCarley; Dimitris Nikitopoulos; Michael C Murphy; Steven A Soper
Journal:  J Am Chem Soc       Date:  2008-06-17       Impact factor: 15.419
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  14 in total

Review 1.  Materials and microfluidics: enabling the efficient isolation and analysis of circulating tumour cells.

Authors:  Joshua M Jackson; Małgorzata A Witek; Joyce W Kamande; Steven A Soper
Journal:  Chem Soc Rev       Date:  2017-07-17       Impact factor: 54.564

2.  Visible photorelease of liquid biopsy markers following microfluidic affinity-enrichment.

Authors:  Thilanga N Pahattuge; J Matt Jackson; Rane Digamber; Harshani Wijerathne; Virginia Brown; Malgorzata A Witek; Chamani Perera; Richard S Givens; Blake R Peterson; Steven A Soper
Journal:  Chem Commun (Camb)       Date:  2020-03-12       Impact factor: 6.222

3.  Time-Delayed Integration-Spectral Flow Cytometer (TDI-SFC) for Low-Abundance-Cell Immunophenotyping.

Authors:  Wenting Hu; Steven A Soper; J Matt Jackson
Journal:  Anal Chem       Date:  2019-03-13       Impact factor: 6.986

4.  Immunofunctional photodegradable poly(ethylene glycol) hydrogel surfaces for the capture and release of rare cells.

Authors:  Paige J LeValley; Mark W Tibbitt; Ben Noren; Prathamesh Kharkar; April M Kloxin; Kristi S Anseth; Mehmet Toner; John Oakey
Journal:  Colloids Surf B Biointerfaces       Date:  2018-11-20       Impact factor: 5.268

5.  Isolation of circulating plasma cells from blood of patients diagnosed with clonal plasma cell disorders using cell selection microfluidics.

Authors:  Joyce W Kamande; Maria A M Lindell; Małgorzata A Witek; Peter M Voorhees; Steven A Soper
Journal:  Integr Biol (Camb)       Date:  2018-02-19       Impact factor: 2.192

6.  Microfluidics for the detection of minimal residual disease in acute myeloid leukemia patients using circulating leukemic cells selected from blood.

Authors:  Joshua M Jackson; James B Taylor; Małgorzata A Witek; Sally A Hunsucker; Jennifer P Waugh; Yuri Fedoriw; Thomas C Shea; Steven A Soper; Paul M Armistead
Journal:  Analyst       Date:  2016-01-21       Impact factor: 4.616

7.  Label-free counting of affinity-enriched circulating tumor cells (CTCs) using a thermoplastic micro-Coulter counter (μCC).

Authors:  Cong Kong; Mengjia Hu; Kumuditha M Weerakoon-Ratnayake; Malgorzata A Witek; Kavya Dathathreya; Mateusz L Hupert; Steven A Soper
Journal:  Analyst       Date:  2020-03-02       Impact factor: 4.616

8.  Circulating Tumor Cell Detection In Epithelial Ovarian Cancer Using Dual-Component Antibodies Targeting EpCAM And FRα.

Authors:  Na Li; Hao Zuo; Luojun Chen; Huali Liu; Jin Zhou; Yi Yao; Bin Xu; Hongyun Gong; Yiming Weng; Qinyong Hu; Qibin Song; Min Peng; Yanxiang Cheng
Journal:  Cancer Manag Res       Date:  2019-12-31       Impact factor: 3.989

9.  Combined use of EpCAM and FRα enables the high-efficiency capture of circulating tumor cells in non-small cell lung cancer.

Authors:  Luojun Chen; Min Peng; Na Li; Qibin Song; Yi Yao; Bin Xu; Huali Liu; Peng Ruan
Journal:  Sci Rep       Date:  2018-01-19       Impact factor: 4.379

10.  Aptamer-Mediated Transparent-Biocompatible Nanostructured Surfaces for Hepotocellular Circulating Tumor Cells Enrichment.

Authors:  Shuyi Wang; Chunxiao Zhang; Guozhou Wang; Boran Cheng; Yulei Wang; Fangfang Chen; Yuanyuan Chen; Maohui Feng; Bin Xiong
Journal:  Theranostics       Date:  2016-08-07       Impact factor: 11.556
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