Erin M Miller1, Jorge E Quintero1, François Pomerleau1, Peter Huettl1, Greg A Gerhardt2, Paul E A Glaser3. 1. Department of Anatomy & Neurobiology, University of Kentucky Chandler Medical Center, Lexington, KY 40536, USA; Center for Microelectrode Technology, University of Kentucky Chandler Medical Center, Lexington, KY 40536, USA; Parkinson's Disease Translational Research Center of Excellence, University of Kentucky Chandler Medical Center, Lexington, KY 40536, USA. 2. Department of Anatomy & Neurobiology, University of Kentucky Chandler Medical Center, Lexington, KY 40536, USA; Department of Psychiatry, University of Kentucky Chandler Medical Center, Lexington, KY 40536, USA; Department of Neurology, University of Kentucky Chandler Medical Center, Lexington, KY 40536, USA; Department of Neurosurgery, University of Kentucky Chandler Medical Center, Lexington, KY 40536, USA; Center for Microelectrode Technology, University of Kentucky Chandler Medical Center, Lexington, KY 40536, USA; Parkinson's Disease Translational Research Center of Excellence, University of Kentucky Chandler Medical Center, Lexington, KY 40536, USA. 3. Department of Anatomy & Neurobiology, University of Kentucky Chandler Medical Center, Lexington, KY 40536, USA; Department of Pediatrics, University of Kentucky Chandler Medical Center, Lexington, KY 40536, USA; Department of Psychiatry, University of Kentucky Chandler Medical Center, Lexington, KY 40536, USA; Center for Microelectrode Technology, University of Kentucky Chandler Medical Center, Lexington, KY 40536, USA; Parkinson's Disease Translational Research Center of Excellence, University of Kentucky Chandler Medical Center, Lexington, KY 40536, USA. Electronic address: pglas0@uky.edu.
Abstract
BACKGROUND: The aberrant regulation of glutamate has been implicated in numerous psychiatric disorders including drug addiction and attention-deficit/hyperactivity disorder. To understand glutamate signaling and its role in facilitating disease, tools to directly measure glutamate in a complex, neural network are needed. NEW METHOD: The development of a ceramic-based, dual-sided, biomorphic microelectrode array with four recording sites on each side to facilitate a more detailed measurement of glutamate in awake, behaving rodents. RESULTS: In vitro calibrations of these biosensors showed selective and specific responses to glutamate. In awake rats, these biomorphic electrode arrays enabled the concurrent evaluation of glutamate in a network, the frontal cortex: including the cingulate, prelimbic, infralimbic and dorsal peduncle regions. Regions within the frontal cortex exhibited varying phasic glutamate patterns in awake animals.Comparison with existing method: Existing methodologies to measure glutamate neurotransmission employ single-sided biosensors or biosensors capable of measuring neurochemicals at only one location in space. CONCLUSIONS: Multi-site, biomorphic neurochemical biosensors provide a method for simultaneously measuring glutamate in multiple areas of a neural network in the brain. Published by Elsevier B.V.
BACKGROUND: The aberrant regulation of glutamate has been implicated in numerous psychiatric disorders including drug addiction and attention-deficit/hyperactivity disorder. To understand glutamate signaling and its role in facilitating disease, tools to directly measure glutamate in a complex, neural network are needed. NEW METHOD: The development of a ceramic-based, dual-sided, biomorphic microelectrode array with four recording sites on each side to facilitate a more detailed measurement of glutamate in awake, behaving rodents. RESULTS: In vitro calibrations of these biosensors showed selective and specific responses to glutamate. In awake rats, these biomorphic electrode arrays enabled the concurrent evaluation of glutamate in a network, the frontal cortex: including the cingulate, prelimbic, infralimbic and dorsal peduncle regions. Regions within the frontal cortex exhibited varying phasic glutamate patterns in awake animals.Comparison with existing method: Existing methodologies to measure glutamate neurotransmission employ single-sided biosensors or biosensors capable of measuring neurochemicals at only one location in space. CONCLUSIONS: Multi-site, biomorphic neurochemical biosensors provide a method for simultaneously measuring glutamate in multiple areas of a neural network in the brain. Published by Elsevier B.V.
Authors: Pradoldej Sompol; Jennifer L Furman; Melanie M Pleiss; Susan D Kraner; Irina A Artiushin; Seth R Batten; Jorge E Quintero; Linda A Simmerman; Tina L Beckett; Mark A Lovell; M Paul Murphy; Greg A Gerhardt; Christopher M Norris Journal: J Neurosci Date: 2017-05-30 Impact factor: 6.167
Authors: Corwin R Butler; Jeffery A Boychuk; Francois Pomerleau; Ramona Alcala; Peter Huettl; Yi Ai; Johan Jakobsson; Sidney W Whiteheart; Greg A Gerhardt; Bret N Smith; John T Slevin Journal: Epilepsy Res Date: 2019-11-26 Impact factor: 3.045