| Literature DB >> 25610957 |
Yinghua Xu1, Yajun Tan, Catpagavalli Asokanathan, Shumin Zhang, Dorothy Xing, Junzhi Wang.
Abstract
Whole-cell pertussis vaccines (WPVs) have been completely replaced by the co-purified acellular vaccines (APVs) in China. To date few laboratory studies were reported for co-purified APVs in terms of their antigenic composition and protective immune responses. To further understand the antigenic composition in co-purified APVs, in the present study 2-dimensional gel electrophoresis-based proteomic technology was used to analyze the composition of co-purified APVs. The results showed that besides the main antigens pertussis toxin (PT) and filamentous hemagglutinin (FHA), co-purified APVs also contained pertactin (PRN), fimbriae (FIM) 2and3 and other minor protein antigens. Of the 9 proteins identified, 3 were differentially presented in products from manufacturer 1 and manufacturer 2. Compared with WPVs and purified APVs, co-purified APVs induced a mixed Th1/Th2 immune response with more toward to a Th1 response than the purified APVs in this study. These results hint that different immune mechanisms might be involved in protection induced by co-purified and purified APVs.Entities:
Keywords: 2-DE, 2-dimensional gel electrophoresis; APVs, acellular pertussis vaccines; BipA, putative outer membrane ligand binding protein; Co-purified acellular pertussis vaccines; FHA, filamentous hemagglutinin; FIM, fimbriae; PRN, pertactin; PT, pertussis toxin; SHD, single human dose; Sbp, sulfate-binding protein precursor; WPVs, whole-cell pertussis vaccines; bvg, Bordetella virulence regulon; immune responses; protection; proteomic analysis; sphB1, autotransporter subtilisin-like protease
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Year: 2015 PMID： 25610957 PMCID： PMC4514368 DOI： 10.4161/21645515.2014.988549
Source DB: PubMed Journal: Hum Vaccin Immunother ISSN： 2164-5515 Impact factor: 3.452