[Formation of p-tyramine from dopamine bound to synaptic receptors of the rat brain in vitro].

Tyramine (TA) revealed earlier during the functioning of dopamine (DA)-receptors of the rat brain (after learning) in vivo was produced from dopamine bound by DA-receptors of the synaptic membranes in the system which was exposed to the influence of the microdischarge electroradialysis in vitro. It is shown that the formation of p-TA under these conditions depends on the period of the micro-discharge effect on the system, it is maximal at exposition of 30 s for I = 4.2 mA. In control solutions of standard DA and DA preincubated with the membranes of the cerebellum homogenate, without DA-receptors, p-TA was not revealed under these conditions. The results obtained confirm the supposition that p-TA is the product of the DA-receptors functioning in vivo.