Serap Yildirim1, Abdulkadir Yildirim2, Senol Dane3, Elvin Aliyev2, Ramazan Yigitoglu4. 1. Department of Physiology, Faculty of Medicine, Ataturk University, Erzurum, Turkey. 2. Department of Biochemistry, Faculty of Medicine, Ataturk University, Erzurum, Turkey. 3. Department of Physiology, Faculty of Medicine, Fatih University, Ankara, Turkey. 4. Department of Biochemistry, Faculty of Medicine, Fatih University, Ankara, Turkey.
Abstract
OBJECTIVE: The present study was designed to investigate the dose-dependent protective effect of L-carnitine (LC) on thyroid hormone-induced oxidative stress in rat liver tissue. MATERIALS AND METHODS: Twenty-one male Sprague Dawley rats were divided into four groups: control, hyperthyroidism, hyperthyroidism plus L-carnitine 100, and hyperthyroidism plus L-carnitine 500. Hyperthyroidism was induced in rats by injecting 250 μg of L-thyroxine/kg body weight/day for twenty consecutive days. The activities of catalase (CAT), glutathione peroxidase (GPX) and myeloperoxidase (MPO) and the level of malondialdehyde (MDA) were measured in liver homogenates. RESULTS: The liver CAT, GPX and MPO activities were significantly lower in the hyperthyroid rats than in the control group. Treating hyperthyroid rats with both low-dose (100 mg/kg) and high-dose (500 mg/kg) L-carnitine for 10 days resulted in a marked increase in the activities of the antioxidant enzymes in the liver tissue. CONCLUSION: The present study indicates that the low-dose L-carnitine application was sufficient to prevent L-thyroxine-induced oxidative stress in rat livers.
OBJECTIVE: The present study was designed to investigate the dose-dependent protective effect of L-carnitine (LC) on thyroid hormone-induced oxidative stress in rat liver tissue. MATERIALS AND METHODS: Twenty-one male Sprague Dawley rats were divided into four groups: control, hyperthyroidism, hyperthyroidism plus L-carnitine 100, and hyperthyroidism plus L-carnitine 500. Hyperthyroidism was induced in rats by injecting 250 μg of L-thyroxine/kg body weight/day for twenty consecutive days. The activities of catalase (CAT), glutathione peroxidase (GPX) and myeloperoxidase (MPO) and the level of malondialdehyde (MDA) were measured in liver homogenates. RESULTS: The liver CAT, GPX and MPO activities were significantly lower in the hyperthyroid rats than in the control group. Treating hyperthyroid rats with both low-dose (100 mg/kg) and high-dose (500 mg/kg) L-carnitine for 10 days resulted in a marked increase in the activities of the antioxidant enzymes in the liver tissue. CONCLUSION: The present study indicates that the low-dose L-carnitine application was sufficient to prevent L-thyroxine-induced oxidative stress in rat livers.
Authors: Abdulaziz M Aleisa; Abdulhakeem A Al-Majed; Abdulaziz A Al-Yahya; Salim S Al-Rejaie; Saleh A Bakheet; Othman A Al-Shabanah; Mohamed M Sayed-Ahmed Journal: Clin Exp Pharmacol Physiol Date: 2007-12 Impact factor: 2.557
Authors: Barbara Rybus-Kalinowska; Krystyna Zwirska-Korczala; Mariusz Kalinowski; Michał Kukla; Ewa Birkner; Jerzy Jochem Journal: Pol Arch Med Wewn Date: 2008 Jul-Aug